Characterization of Progenitor Cells during Canine Retinal Development
We identify the presence of progenitor cells during retinal development in the dog, as this species represents a natural model for studying several breed-specific degenerative retinal disorders. Antibodies to detected progenitor cells (Pax6, C-kit, and nestin) and ganglion cells (BDNF, Brn3a, and Th...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2012/675805 |
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| author | Mallely Ávila-García Gustavo García-Sánchez Esmeralda Lira-Romero Norma Moreno-Mendoza |
| author_facet | Mallely Ávila-García Gustavo García-Sánchez Esmeralda Lira-Romero Norma Moreno-Mendoza |
| author_sort | Mallely Ávila-García |
| collection | DOAJ |
| description | We identify the presence of progenitor cells during retinal development in the dog, as this species represents a natural model for studying several breed-specific degenerative retinal disorders. Antibodies to detected progenitor cells (Pax6, C-kit, and nestin) and ganglion cells (BDNF, Brn3a, and Thy1) were used in combination with H3 for the purpose of identifying proliferating cells. Pax6, nestin, C-kit, and H3 were localized mainly in the neuroblastic layer of the retina during the embryonic stage. During the fetal stage, proteins were expressed in the inner neuroblastic layer (INL) as well as in the outer neuroblastic layer; BDNF, Thy1, and Brn3a were also expressed in the INL. During the neonatal stage only C-kit was not expressed. Proliferating cells were present in both undifferentiated and differentiated retina. These results suggest that, during canine retinogenesis, progenitor cells are distributed along the retina and some of these cells remain as progenitor cells of the ganglion cells during the first postnatal days. |
| format | Article |
| id | doaj-art-d528880fff0e4e0ca4fe48ff5c283ad6 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-d528880fff0e4e0ca4fe48ff5c283ad62025-02-03T05:46:23ZengWileyStem Cells International1687-966X1687-96782012-01-01201210.1155/2012/675805675805Characterization of Progenitor Cells during Canine Retinal DevelopmentMallely Ávila-García0Gustavo García-Sánchez1Esmeralda Lira-Romero2Norma Moreno-Mendoza3Departamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Apartado Postal 70228, Ciudad de México, DF 04510, MexicoSchool of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USADepartamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Apartado Postal 70228, Ciudad de México, DF 04510, MexicoDepartamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, Apartado Postal 70228, Ciudad de México, DF 04510, MexicoWe identify the presence of progenitor cells during retinal development in the dog, as this species represents a natural model for studying several breed-specific degenerative retinal disorders. Antibodies to detected progenitor cells (Pax6, C-kit, and nestin) and ganglion cells (BDNF, Brn3a, and Thy1) were used in combination with H3 for the purpose of identifying proliferating cells. Pax6, nestin, C-kit, and H3 were localized mainly in the neuroblastic layer of the retina during the embryonic stage. During the fetal stage, proteins were expressed in the inner neuroblastic layer (INL) as well as in the outer neuroblastic layer; BDNF, Thy1, and Brn3a were also expressed in the INL. During the neonatal stage only C-kit was not expressed. Proliferating cells were present in both undifferentiated and differentiated retina. These results suggest that, during canine retinogenesis, progenitor cells are distributed along the retina and some of these cells remain as progenitor cells of the ganglion cells during the first postnatal days.http://dx.doi.org/10.1155/2012/675805 |
| spellingShingle | Mallely Ávila-García Gustavo García-Sánchez Esmeralda Lira-Romero Norma Moreno-Mendoza Characterization of Progenitor Cells during Canine Retinal Development Stem Cells International |
| title | Characterization of Progenitor Cells during Canine Retinal Development |
| title_full | Characterization of Progenitor Cells during Canine Retinal Development |
| title_fullStr | Characterization of Progenitor Cells during Canine Retinal Development |
| title_full_unstemmed | Characterization of Progenitor Cells during Canine Retinal Development |
| title_short | Characterization of Progenitor Cells during Canine Retinal Development |
| title_sort | characterization of progenitor cells during canine retinal development |
| url | http://dx.doi.org/10.1155/2012/675805 |
| work_keys_str_mv | AT mallelyavilagarcia characterizationofprogenitorcellsduringcanineretinaldevelopment AT gustavogarciasanchez characterizationofprogenitorcellsduringcanineretinaldevelopment AT esmeraldaliraromero characterizationofprogenitorcellsduringcanineretinaldevelopment AT normamorenomendoza characterizationofprogenitorcellsduringcanineretinaldevelopment |