Male-specific lethal 1 (MSL1) promotes Erastin-induced ferroptosis in colon cancer cells by regulating the KCTD12-SLC7A11 axis
Abstract MSL1, a scaffold protein of the MSL histone acetyltransferase complex, is crucial for its structural integrity and enzymatic activity. While MSL1 is highly expressed in various tumors, its role in tumor progression and cell death remains unclear. Here, we provide evidence of a negative regu...
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2025-04-01
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| Series: | Cell Death and Disease |
| Online Access: | https://doi.org/10.1038/s41419-025-07555-7 |
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| author | Lifu Luo Qingzhi Zhao Xueli Cui Shijiao Dong Yong Wang Nan Jiang Chengyu Cai Jingji Jin Bing Liang |
| author_facet | Lifu Luo Qingzhi Zhao Xueli Cui Shijiao Dong Yong Wang Nan Jiang Chengyu Cai Jingji Jin Bing Liang |
| author_sort | Lifu Luo |
| collection | DOAJ |
| description | Abstract MSL1, a scaffold protein of the MSL histone acetyltransferase complex, is crucial for its structural integrity and enzymatic activity. While MSL1 is highly expressed in various tumors, its role in tumor progression and cell death remains unclear. Here, we provide evidence of a negative regulatory relationship between MSL1 and KCTD12 through biochemical assays and knockdown/overexpression studies. Notably, in colon cancer cells, the ferroptosis inducer Erastin significantly suppressed MSL1 expression, leading to KCTD12 upregulation. Moreover, MSL1 promotes Erastin-induced ferroptosis in HCT116 and SW480 cells via the KCTD12-SLC7A11 axis. Consistently, Erastin-induced changes in ROS, GSH, and MDA levels were regulated by this axis, highlighting its role in ferroptosis. These findings offer potential therapeutic targets and a theoretical foundation for colon cancer treatment. |
| format | Article |
| id | doaj-art-d523c6ea43364d31919dca30d88b03ac |
| institution | DOAJ |
| issn | 2041-4889 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Cell Death and Disease |
| spelling | doaj-art-d523c6ea43364d31919dca30d88b03ac2025-08-20T03:06:51ZengNature Publishing GroupCell Death and Disease2041-48892025-04-0116111210.1038/s41419-025-07555-7Male-specific lethal 1 (MSL1) promotes Erastin-induced ferroptosis in colon cancer cells by regulating the KCTD12-SLC7A11 axisLifu Luo0Qingzhi Zhao1Xueli Cui2Shijiao Dong3Yong Wang4Nan Jiang5Chengyu Cai6Jingji Jin7Bing Liang8Department of Ophthalmology, The Second Hospital of Jilin UniversitySchool of Nursing, Jilin UniversitySchool of Life Sciences, Jilin UniversitySchool of Life Sciences, Jilin UniversityUrology Department, Jilin Province People’s HospitalDepartment of Cardiovascular center, Jilin University First HospitalSchool of Life Sciences, Jilin UniversitySchool of Life Sciences, Jilin UniversitySchool of Nursing, Jilin UniversityAbstract MSL1, a scaffold protein of the MSL histone acetyltransferase complex, is crucial for its structural integrity and enzymatic activity. While MSL1 is highly expressed in various tumors, its role in tumor progression and cell death remains unclear. Here, we provide evidence of a negative regulatory relationship between MSL1 and KCTD12 through biochemical assays and knockdown/overexpression studies. Notably, in colon cancer cells, the ferroptosis inducer Erastin significantly suppressed MSL1 expression, leading to KCTD12 upregulation. Moreover, MSL1 promotes Erastin-induced ferroptosis in HCT116 and SW480 cells via the KCTD12-SLC7A11 axis. Consistently, Erastin-induced changes in ROS, GSH, and MDA levels were regulated by this axis, highlighting its role in ferroptosis. These findings offer potential therapeutic targets and a theoretical foundation for colon cancer treatment.https://doi.org/10.1038/s41419-025-07555-7 |
| spellingShingle | Lifu Luo Qingzhi Zhao Xueli Cui Shijiao Dong Yong Wang Nan Jiang Chengyu Cai Jingji Jin Bing Liang Male-specific lethal 1 (MSL1) promotes Erastin-induced ferroptosis in colon cancer cells by regulating the KCTD12-SLC7A11 axis Cell Death and Disease |
| title | Male-specific lethal 1 (MSL1) promotes Erastin-induced ferroptosis in colon cancer cells by regulating the KCTD12-SLC7A11 axis |
| title_full | Male-specific lethal 1 (MSL1) promotes Erastin-induced ferroptosis in colon cancer cells by regulating the KCTD12-SLC7A11 axis |
| title_fullStr | Male-specific lethal 1 (MSL1) promotes Erastin-induced ferroptosis in colon cancer cells by regulating the KCTD12-SLC7A11 axis |
| title_full_unstemmed | Male-specific lethal 1 (MSL1) promotes Erastin-induced ferroptosis in colon cancer cells by regulating the KCTD12-SLC7A11 axis |
| title_short | Male-specific lethal 1 (MSL1) promotes Erastin-induced ferroptosis in colon cancer cells by regulating the KCTD12-SLC7A11 axis |
| title_sort | male specific lethal 1 msl1 promotes erastin induced ferroptosis in colon cancer cells by regulating the kctd12 slc7a11 axis |
| url | https://doi.org/10.1038/s41419-025-07555-7 |
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