ANTIBACTERIAL ACTIVITY OF MANGOSTEEN BARK FRACTION (GARCINIA MANGOSTANA L.) SALMONELLA TYPHI ATCC 13311
Background: Typhoid fever is caused by Salmonella typhi bacterial infection which spreads via the faecal-oral route and has epidemic potential. Salmonella typhi is a Gram-negative bacterium in the Enterobacteriaceae family. Currently, the use of natural ingredients can be an alternative treatment fo...
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Universitas Airlangga
2022-03-01
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| Series: | Journal of Vocational Health Studies |
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| Online Access: | https://e-journal.unair.ac.id/JVHS/article/view/33309/17301 |
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| author | Salmah Wilujeng Anggraini Vevi Maritha Susanti Erikania |
| author_facet | Salmah Wilujeng Anggraini Vevi Maritha Susanti Erikania |
| author_sort | Salmah Wilujeng Anggraini |
| collection | DOAJ |
| description | Background: Typhoid fever is caused by Salmonella typhi bacterial infection which spreads via the faecal-oral route and has epidemic potential. Salmonella typhi is a Gram-negative bacterium in the Enterobacteriaceae family. Currently, the use of natural ingredients can be an alternative treatment for typhoid fever, one of which is the mangosteen stem skin (Garcinia mangostana L.). Purpose: To determine the antibacterial activity of mangosteen stem skin fraction against Salmonella typhi ATCC 13311. Method:The maceration method was selected using methanol solvent and fractionation by ECC using ethyl acetate and n-hexane as solvents. This study used five treatments (fraction with a concentration of 20%, 40%, 80%, chloramphenicol 30µg as a positive control, and DMSO as a negative control) which tested their antibacterial activity against Salmonella typhi ATCC 13311 by disc diffusion. Result: The results showed that the ethyl acetate fraction of mangosteen stem skin had the greatest average inhibition zone, that is 13.86 ± 0.72 mm compared to the n-hexane fraction of mangosteen stem skin with an inhibition zone of 10.43 ± 0.73 mm. The data obtained were then analyzed with One-way ANOVA. Conclusion: The results obtained were p=0.000 (sig <0.05), indicating that there was a significant difference between the ethyl acetate fraction and the n-hexane fraction with 20%, 60%, and 80% concentrations with positive control |
| format | Article |
| id | doaj-art-d51e2abc42d343b2a029d87e190751c5 |
| institution | Kabale University |
| issn | 2580-7161 2580-717X |
| language | English |
| publishDate | 2022-03-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Journal of Vocational Health Studies |
| spelling | doaj-art-d51e2abc42d343b2a029d87e190751c52025-02-02T06:16:23ZengUniversitas AirlanggaJournal of Vocational Health Studies2580-71612580-717X2022-03-015313914510.20473/jvhs.V5.I3.2022.139-145ANTIBACTERIAL ACTIVITY OF MANGOSTEEN BARK FRACTION (GARCINIA MANGOSTANA L.) SALMONELLA TYPHI ATCC 13311Salmah Wilujeng Anggraini0https://orcid.org/0000-0002-9050-0089Vevi Maritha1https://orcid.org/0000-0002-3697-7513Susanti Erikania2https://orcid.org/0000-0002-6338-3710 StiKes Bhakti Husada Mulia MadiunStiKes Bhakti Husada Mulia MadiunStiKes Bhakti Husada Mulia MadiunBackground: Typhoid fever is caused by Salmonella typhi bacterial infection which spreads via the faecal-oral route and has epidemic potential. Salmonella typhi is a Gram-negative bacterium in the Enterobacteriaceae family. Currently, the use of natural ingredients can be an alternative treatment for typhoid fever, one of which is the mangosteen stem skin (Garcinia mangostana L.). Purpose: To determine the antibacterial activity of mangosteen stem skin fraction against Salmonella typhi ATCC 13311. Method:The maceration method was selected using methanol solvent and fractionation by ECC using ethyl acetate and n-hexane as solvents. This study used five treatments (fraction with a concentration of 20%, 40%, 80%, chloramphenicol 30µg as a positive control, and DMSO as a negative control) which tested their antibacterial activity against Salmonella typhi ATCC 13311 by disc diffusion. Result: The results showed that the ethyl acetate fraction of mangosteen stem skin had the greatest average inhibition zone, that is 13.86 ± 0.72 mm compared to the n-hexane fraction of mangosteen stem skin with an inhibition zone of 10.43 ± 0.73 mm. The data obtained were then analyzed with One-way ANOVA. Conclusion: The results obtained were p=0.000 (sig <0.05), indicating that there was a significant difference between the ethyl acetate fraction and the n-hexane fraction with 20%, 60%, and 80% concentrations with positive controlhttps://e-journal.unair.ac.id/JVHS/article/view/33309/17301anti-bacterialfractiongarcinia mangostanasalmonella typhi |
| spellingShingle | Salmah Wilujeng Anggraini Vevi Maritha Susanti Erikania ANTIBACTERIAL ACTIVITY OF MANGOSTEEN BARK FRACTION (GARCINIA MANGOSTANA L.) SALMONELLA TYPHI ATCC 13311 Journal of Vocational Health Studies anti-bacterial fraction garcinia mangostana salmonella typhi |
| title | ANTIBACTERIAL ACTIVITY OF MANGOSTEEN BARK FRACTION (GARCINIA MANGOSTANA L.) SALMONELLA TYPHI ATCC 13311 |
| title_full | ANTIBACTERIAL ACTIVITY OF MANGOSTEEN BARK FRACTION (GARCINIA MANGOSTANA L.) SALMONELLA TYPHI ATCC 13311 |
| title_fullStr | ANTIBACTERIAL ACTIVITY OF MANGOSTEEN BARK FRACTION (GARCINIA MANGOSTANA L.) SALMONELLA TYPHI ATCC 13311 |
| title_full_unstemmed | ANTIBACTERIAL ACTIVITY OF MANGOSTEEN BARK FRACTION (GARCINIA MANGOSTANA L.) SALMONELLA TYPHI ATCC 13311 |
| title_short | ANTIBACTERIAL ACTIVITY OF MANGOSTEEN BARK FRACTION (GARCINIA MANGOSTANA L.) SALMONELLA TYPHI ATCC 13311 |
| title_sort | antibacterial activity of mangosteen bark fraction garcinia mangostana l salmonella typhi atcc 13311 |
| topic | anti-bacterial fraction garcinia mangostana salmonella typhi |
| url | https://e-journal.unair.ac.id/JVHS/article/view/33309/17301 |
| work_keys_str_mv | AT salmahwilujenganggraini antibacterialactivityofmangosteenbarkfractiongarciniamangostanalsalmonellatyphiatcc13311 AT vevimaritha antibacterialactivityofmangosteenbarkfractiongarciniamangostanalsalmonellatyphiatcc13311 AT susantierikania antibacterialactivityofmangosteenbarkfractiongarciniamangostanalsalmonellatyphiatcc13311 |