Genome-Wide Microsatellite Characterization and Molecular Marker Development of Himalayan Griffon (<i>Gyps himalayensis</i>)
The Himalayan griffon (<i>Gyps himalayensis</i>), an obligate scavenging bird in the Qinghai–Tibet Plateau, provides significant ecological services but is currently listed as near threatened. Despite their importance for conservation, genome-wide screening for microsatellites (or SSRs)...
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2025-05-01
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| author | Weibin Guo Dianhua Ke Changcao Wang Haiying Fan |
| author_facet | Weibin Guo Dianhua Ke Changcao Wang Haiying Fan |
| author_sort | Weibin Guo |
| collection | DOAJ |
| description | The Himalayan griffon (<i>Gyps himalayensis</i>), an obligate scavenging bird in the Qinghai–Tibet Plateau, provides significant ecological services but is currently listed as near threatened. Despite their importance for conservation, genome-wide screening for microsatellites (or SSRs) in <i>G. himalayensis</i> and the development of corresponding molecular markers have been lacking. This study presents the first in-depth characterization of genome-wide microsatellites in <i>G. himalayensis</i> and the successful development of polymorphic SSR markers. A total of 240,741 microsatellite loci were detected in the <i>G. himalayensis</i> genome, with an average density of 202.2 SSRs per Mb, accounting for 0.44% of the genome. Mononucleotide repeats (53.2%) were the most prevalent among the different microsatellite motif types. Additionally, 100 primer pairs were initially identified, with 17 found to be polymorphic markers. These loci hold significant potential for revealing genotypic diversity in <i>G. himalayensis</i>, thereby laying a foundation for the conservation of this species. |
| format | Article |
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| institution | OA Journals |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-05-01 |
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| spelling | doaj-art-d513a7140df542b7905060134bf3fac12025-08-20T02:33:39ZengMDPI AGAnimals2076-26152025-05-011510143810.3390/ani15101438Genome-Wide Microsatellite Characterization and Molecular Marker Development of Himalayan Griffon (<i>Gyps himalayensis</i>)Weibin Guo0Dianhua Ke1Changcao Wang2Haiying Fan3School of Life Sciences, Jinggangshan University, Ji’an 343009, ChinaSchool of Life Sciences, Jinggangshan University, Ji’an 343009, ChinaState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, ChinaSchool of Life Sciences, Jinggangshan University, Ji’an 343009, ChinaThe Himalayan griffon (<i>Gyps himalayensis</i>), an obligate scavenging bird in the Qinghai–Tibet Plateau, provides significant ecological services but is currently listed as near threatened. Despite their importance for conservation, genome-wide screening for microsatellites (or SSRs) in <i>G. himalayensis</i> and the development of corresponding molecular markers have been lacking. This study presents the first in-depth characterization of genome-wide microsatellites in <i>G. himalayensis</i> and the successful development of polymorphic SSR markers. A total of 240,741 microsatellite loci were detected in the <i>G. himalayensis</i> genome, with an average density of 202.2 SSRs per Mb, accounting for 0.44% of the genome. Mononucleotide repeats (53.2%) were the most prevalent among the different microsatellite motif types. Additionally, 100 primer pairs were initially identified, with 17 found to be polymorphic markers. These loci hold significant potential for revealing genotypic diversity in <i>G. himalayensis</i>, thereby laying a foundation for the conservation of this species.https://www.mdpi.com/2076-2615/15/10/1438avian scavengersvulturesgenomic SSRspolymorphismmolecular marker |
| spellingShingle | Weibin Guo Dianhua Ke Changcao Wang Haiying Fan Genome-Wide Microsatellite Characterization and Molecular Marker Development of Himalayan Griffon (<i>Gyps himalayensis</i>) Animals avian scavengers vultures genomic SSRs polymorphism molecular marker |
| title | Genome-Wide Microsatellite Characterization and Molecular Marker Development of Himalayan Griffon (<i>Gyps himalayensis</i>) |
| title_full | Genome-Wide Microsatellite Characterization and Molecular Marker Development of Himalayan Griffon (<i>Gyps himalayensis</i>) |
| title_fullStr | Genome-Wide Microsatellite Characterization and Molecular Marker Development of Himalayan Griffon (<i>Gyps himalayensis</i>) |
| title_full_unstemmed | Genome-Wide Microsatellite Characterization and Molecular Marker Development of Himalayan Griffon (<i>Gyps himalayensis</i>) |
| title_short | Genome-Wide Microsatellite Characterization and Molecular Marker Development of Himalayan Griffon (<i>Gyps himalayensis</i>) |
| title_sort | genome wide microsatellite characterization and molecular marker development of himalayan griffon i gyps himalayensis i |
| topic | avian scavengers vultures genomic SSRs polymorphism molecular marker |
| url | https://www.mdpi.com/2076-2615/15/10/1438 |
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