INVESTIGATION OF INFECTIOUS LARYNGEOTRACHEITIS VIRUS IN IRAQI CHICKEN FARMS

Twenty samples of Laryngeal and Tracheal tissues from laying chickens of two flocks with suspicious infectious laryngeotracheitis were tested for the detection of the causative virus by using virus isolation in chicken embryo fibroblast (CEF)cell culture and chicken embryos by chorioallantoic membra...

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Main Authors: Aida Bara Allawe, Shony M.Odisho, Salameh Barhoom
Format: Article
Language:English
Published: College of Veterinary Medicine, University of Basrah, Iraq 2015-06-01
Series:Basrah Journal of Veterinary Research
Subjects:
Online Access:https://bjvr.uobasrah.edu.iq/article_102447_8d51408f58ba90638da86a1f8b24eb21.pdf
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author Aida Bara Allawe
Shony M.Odisho
Salameh Barhoom
author_facet Aida Bara Allawe
Shony M.Odisho
Salameh Barhoom
author_sort Aida Bara Allawe
collection DOAJ
description Twenty samples of Laryngeal and Tracheal tissues from laying chickens of two flocks with suspicious infectious laryngeotracheitis were tested for the detection of the causative virus by using virus isolation in chicken embryo fibroblast (CEF)cell culture and chicken embryos by chorioallantoic membrane inoculation and conventional polymerase chain reaction(PCR).The virus was isolated from three samples (larynx and trachea) out of ten samples at Al-Sawara city on (CEF)cell culture and produce pock lesions on chorioallantoic membrane of infected chicken embryos in which the virus was isolated. Positive PCR results were detected in the three clinical samples, isolated virus in CEF cell culture and isolated virus in chicken embryos (inoculated on chorioallantoic membrane).
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issn 1813-8497
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language English
publishDate 2015-06-01
publisher College of Veterinary Medicine, University of Basrah, Iraq
record_format Article
series Basrah Journal of Veterinary Research
spelling doaj-art-d50e89059f214d52943c47f8a064d9bb2025-08-20T03:49:45ZengCollege of Veterinary Medicine, University of Basrah, IraqBasrah Journal of Veterinary Research1813-84972410-84562015-06-0114130231010.33762/bvetr.2015.102447102447INVESTIGATION OF INFECTIOUS LARYNGEOTRACHEITIS VIRUS IN IRAQI CHICKEN FARMSAida Bara AllaweShony M.OdishoSalameh BarhoomTwenty samples of Laryngeal and Tracheal tissues from laying chickens of two flocks with suspicious infectious laryngeotracheitis were tested for the detection of the causative virus by using virus isolation in chicken embryo fibroblast (CEF)cell culture and chicken embryos by chorioallantoic membrane inoculation and conventional polymerase chain reaction(PCR).The virus was isolated from three samples (larynx and trachea) out of ten samples at Al-Sawara city on (CEF)cell culture and produce pock lesions on chorioallantoic membrane of infected chicken embryos in which the virus was isolated. Positive PCR results were detected in the three clinical samples, isolated virus in CEF cell culture and isolated virus in chicken embryos (inoculated on chorioallantoic membrane).https://bjvr.uobasrah.edu.iq/article_102447_8d51408f58ba90638da86a1f8b24eb21.pdflaryngeotracheitischorioallantoiccef
spellingShingle Aida Bara Allawe
Shony M.Odisho
Salameh Barhoom
INVESTIGATION OF INFECTIOUS LARYNGEOTRACHEITIS VIRUS IN IRAQI CHICKEN FARMS
Basrah Journal of Veterinary Research
laryngeotracheitis
chorioallantoic
cef
title INVESTIGATION OF INFECTIOUS LARYNGEOTRACHEITIS VIRUS IN IRAQI CHICKEN FARMS
title_full INVESTIGATION OF INFECTIOUS LARYNGEOTRACHEITIS VIRUS IN IRAQI CHICKEN FARMS
title_fullStr INVESTIGATION OF INFECTIOUS LARYNGEOTRACHEITIS VIRUS IN IRAQI CHICKEN FARMS
title_full_unstemmed INVESTIGATION OF INFECTIOUS LARYNGEOTRACHEITIS VIRUS IN IRAQI CHICKEN FARMS
title_short INVESTIGATION OF INFECTIOUS LARYNGEOTRACHEITIS VIRUS IN IRAQI CHICKEN FARMS
title_sort investigation of infectious laryngeotracheitis virus in iraqi chicken farms
topic laryngeotracheitis
chorioallantoic
cef
url https://bjvr.uobasrah.edu.iq/article_102447_8d51408f58ba90638da86a1f8b24eb21.pdf
work_keys_str_mv AT aidabaraallawe investigationofinfectiouslaryngeotracheitisvirusiniraqichickenfarms
AT shonymodisho investigationofinfectiouslaryngeotracheitisvirusiniraqichickenfarms
AT salamehbarhoom investigationofinfectiouslaryngeotracheitisvirusiniraqichickenfarms