Direct synthesis of mycophenolic acid aryl esters with antioxidant and antiproliferative properties

Abstract Naturally occurring phenols were incorporated into the mycophenolic acid (MPA) core to form sixteen new MPA derivatives. Ester conjugates of MPA with isoeugenol, methyl o-coumarate, and raspberry ketone exhibited the highest antioxidant activity in the DPPH test. These derivatives were then...

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Main Authors: Juliusz Walczak, Dorota Iwaszkiewicz-Grześ, Magdalena Śliwka-Kaszyńska, Agnieszka Kurdyn, Ewa Augustin, Agnieszka Viapiana, Alina Plenis, Grzegorz Cholewiński
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Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-11871-5
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author Juliusz Walczak
Dorota Iwaszkiewicz-Grześ
Magdalena Śliwka-Kaszyńska
Agnieszka Kurdyn
Ewa Augustin
Agnieszka Viapiana
Alina Plenis
Grzegorz Cholewiński
author_facet Juliusz Walczak
Dorota Iwaszkiewicz-Grześ
Magdalena Śliwka-Kaszyńska
Agnieszka Kurdyn
Ewa Augustin
Agnieszka Viapiana
Alina Plenis
Grzegorz Cholewiński
author_sort Juliusz Walczak
collection DOAJ
description Abstract Naturally occurring phenols were incorporated into the mycophenolic acid (MPA) core to form sixteen new MPA derivatives. Ester conjugates of MPA with isoeugenol, methyl o-coumarate, and raspberry ketone exhibited the highest antioxidant activity in the DPPH test. These derivatives were then tested against the human pancreatic cancer AsPC-1 cells, showing cytotoxicity similar to that of the referential parent compounds MPA and mycophenolate mofetil (MMF). Subsequently, most of the obtained MPA esters proved activity against the T-Jurkat cells and peripheral blood mononuclear cells (PBMCs), serving as an immunosuppressive model, and worked as inosine-5’-monophosphate dehydrogenase (IMPDH) inhibitors. The most promising immunosuppressive conjugates were the esters of MPA with sesamol and raspberry ketone structural motifs, which held the highest selectivity index (SI) for PBMCs, thus serving as a good starting point for new drug development.
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spelling doaj-art-d505fc06761a4d818d54e9461dabdfbc2025-08-20T03:42:38ZengNature PortfolioScientific Reports2045-23222025-07-0115111210.1038/s41598-025-11871-5Direct synthesis of mycophenolic acid aryl esters with antioxidant and antiproliferative propertiesJuliusz Walczak0Dorota Iwaszkiewicz-Grześ1Magdalena Śliwka-Kaszyńska2Agnieszka Kurdyn3Ewa Augustin4Agnieszka Viapiana5Alina Plenis6Grzegorz Cholewiński7Department of Organic Chemistry, Faculty of Chemistry, Gdańsk University of TechnologyDepartment of Medical Immunology, Faculty of Medicine, Medical University of GdanskDepartment of Organic Chemistry, Faculty of Chemistry, Gdańsk University of TechnologyDepartment of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdańsk University of TechnologyDepartment of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdańsk University of TechnologyDepartment of Analytical Chemistry, Faculty of Pharmacy, Medical University of GdańskDepartment of Analytical Chemistry, Faculty of Pharmacy, Medical University of GdańskDepartment of Organic Chemistry, Faculty of Chemistry, Gdańsk University of TechnologyAbstract Naturally occurring phenols were incorporated into the mycophenolic acid (MPA) core to form sixteen new MPA derivatives. Ester conjugates of MPA with isoeugenol, methyl o-coumarate, and raspberry ketone exhibited the highest antioxidant activity in the DPPH test. These derivatives were then tested against the human pancreatic cancer AsPC-1 cells, showing cytotoxicity similar to that of the referential parent compounds MPA and mycophenolate mofetil (MMF). Subsequently, most of the obtained MPA esters proved activity against the T-Jurkat cells and peripheral blood mononuclear cells (PBMCs), serving as an immunosuppressive model, and worked as inosine-5’-monophosphate dehydrogenase (IMPDH) inhibitors. The most promising immunosuppressive conjugates were the esters of MPA with sesamol and raspberry ketone structural motifs, which held the highest selectivity index (SI) for PBMCs, thus serving as a good starting point for new drug development.https://doi.org/10.1038/s41598-025-11871-5Mycophenolic acidNatural phenolsEsterificationIMPDH inhibitionAntioxidants
spellingShingle Juliusz Walczak
Dorota Iwaszkiewicz-Grześ
Magdalena Śliwka-Kaszyńska
Agnieszka Kurdyn
Ewa Augustin
Agnieszka Viapiana
Alina Plenis
Grzegorz Cholewiński
Direct synthesis of mycophenolic acid aryl esters with antioxidant and antiproliferative properties
Scientific Reports
Mycophenolic acid
Natural phenols
Esterification
IMPDH inhibition
Antioxidants
title Direct synthesis of mycophenolic acid aryl esters with antioxidant and antiproliferative properties
title_full Direct synthesis of mycophenolic acid aryl esters with antioxidant and antiproliferative properties
title_fullStr Direct synthesis of mycophenolic acid aryl esters with antioxidant and antiproliferative properties
title_full_unstemmed Direct synthesis of mycophenolic acid aryl esters with antioxidant and antiproliferative properties
title_short Direct synthesis of mycophenolic acid aryl esters with antioxidant and antiproliferative properties
title_sort direct synthesis of mycophenolic acid aryl esters with antioxidant and antiproliferative properties
topic Mycophenolic acid
Natural phenols
Esterification
IMPDH inhibition
Antioxidants
url https://doi.org/10.1038/s41598-025-11871-5
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