Mutagenicity and DNA damage assessment of locally produced nano-hydroxyapatite-silica-glass ionomer cement on human periodontal ligament fibroblasts using Ames and Comet assays
Aim: This research assessed the mutagenicity and DNA damage of a novel type of nano-hydroxyapatite-silica glass ionomer cement (nano-HA-SiO2-GIC) and a conventional GIC (cGIC) using Ames and Comet assays. Methods: Cell viability was tested on human periodontal ligament fibroblasts (HPLFs) using 3.12...
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Springer
2024-11-01
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| Series: | Saudi Dental Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1013905224002384 |
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| author | Fayez Hussain Niazi Norhayati Luddin Abdurahman Niazy Suharni Mohamad Masitah Hayati Harun Mohammed Noushad Kannan Thirumulu Ponnuraj |
| author_facet | Fayez Hussain Niazi Norhayati Luddin Abdurahman Niazy Suharni Mohamad Masitah Hayati Harun Mohammed Noushad Kannan Thirumulu Ponnuraj |
| author_sort | Fayez Hussain Niazi |
| collection | DOAJ |
| description | Aim: This research assessed the mutagenicity and DNA damage of a novel type of nano-hydroxyapatite-silica glass ionomer cement (nano-HA-SiO2-GIC) and a conventional GIC (cGIC) using Ames and Comet assays. Methods: Cell viability was tested on human periodontal ligament fibroblasts (HPLFs) using 3.125 mg/ml, 6.25, 12.5, 25, 50, 100 and 200 mg/ml, on both types of GICs employing MTT assay. For the Comet assay, HPLFs were treated with IC50, IC25 and IC10 of test materials and the tail moments were measured. In the Ames test, four genotypic variants of strains of Salmonella typhimurium (TA100, TA98, TA1537 and TA1535) and a strain of Escherichia coli (WP2 uvrA) were employed. The material tested was extracted using sterile distilled water (0.2 g per ml) at 37 °C for 72 h. This was considered as 100 %, which was diluted to 50, 25, 12.5 and 6.25 % utilizing sterile distilled water. These five concentrations were incubated with the bacterial strains with and without metabolic activation (S9), along with appropriate positive controls. The number of revertant colonies was used to evaluate the outcome. Results: The highest cell viability (159.4 %) for nano-HA-SiO2-GIC was noticed at 3.125 mg/ml, while the lowest (24.26 %) was observed at 200 mg per ml. IC50, IC25 and IC10 values were 95.27, 51.4 and 20.1 mg/ml for cGIC, 106.9, 55.8 and 22.9 mg/ml for nano-HA-SiO2-GIC, respectively. The IC10 of both test materials showed no significant DNA damage compared to that of the negative control based on the Comet assay. The plate treated with nano-HA-SiO2-GIC showed less than double the average number of revertant colonies compared to that of negative control with regard to the Ames test. Conclusions: It can be concluded that nano-HA-SiO2-GIC is non-mutagenic based on the Ames test and did not cause DNA damage at the lowest concentration of IC10 based on the Comet assay. |
| format | Article |
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| issn | 1013-9052 |
| language | English |
| publishDate | 2024-11-01 |
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| series | Saudi Dental Journal |
| spelling | doaj-art-d503f516beee4e3ea55cd11f60dd00262025-08-20T03:19:42ZengSpringerSaudi Dental Journal1013-90522024-11-0136111432143710.1016/j.sdentj.2024.08.009Mutagenicity and DNA damage assessment of locally produced nano-hydroxyapatite-silica-glass ionomer cement on human periodontal ligament fibroblasts using Ames and Comet assaysFayez Hussain Niazi0Norhayati Luddin1Abdurahman Niazy2Suharni Mohamad3Masitah Hayati Harun4Mohammed Noushad5Kannan Thirumulu Ponnuraj6School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia; Department of Restorative and Prosthetic Dentistry, College of Dentistry, Dar Al Uloom University, Riyadh 13314, Saudi ArabiaSchool of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, MalaysiaDepartment of Oral Medicine and Diagnostic Sciences, College of Dentistry, King Saud University, Riyadh 11495, Saudi ArabiaSchool of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, MalaysiaSchool of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, MalaysiaDepartment of Restorative and Prosthetic Dentistry, College of Dentistry, Dar Al Uloom University, Riyadh 13314, Saudi ArabiaSchool of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia; Human Genome Centre, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia; Corresponding author at: School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.Aim: This research assessed the mutagenicity and DNA damage of a novel type of nano-hydroxyapatite-silica glass ionomer cement (nano-HA-SiO2-GIC) and a conventional GIC (cGIC) using Ames and Comet assays. Methods: Cell viability was tested on human periodontal ligament fibroblasts (HPLFs) using 3.125 mg/ml, 6.25, 12.5, 25, 50, 100 and 200 mg/ml, on both types of GICs employing MTT assay. For the Comet assay, HPLFs were treated with IC50, IC25 and IC10 of test materials and the tail moments were measured. In the Ames test, four genotypic variants of strains of Salmonella typhimurium (TA100, TA98, TA1537 and TA1535) and a strain of Escherichia coli (WP2 uvrA) were employed. The material tested was extracted using sterile distilled water (0.2 g per ml) at 37 °C for 72 h. This was considered as 100 %, which was diluted to 50, 25, 12.5 and 6.25 % utilizing sterile distilled water. These five concentrations were incubated with the bacterial strains with and without metabolic activation (S9), along with appropriate positive controls. The number of revertant colonies was used to evaluate the outcome. Results: The highest cell viability (159.4 %) for nano-HA-SiO2-GIC was noticed at 3.125 mg/ml, while the lowest (24.26 %) was observed at 200 mg per ml. IC50, IC25 and IC10 values were 95.27, 51.4 and 20.1 mg/ml for cGIC, 106.9, 55.8 and 22.9 mg/ml for nano-HA-SiO2-GIC, respectively. The IC10 of both test materials showed no significant DNA damage compared to that of the negative control based on the Comet assay. The plate treated with nano-HA-SiO2-GIC showed less than double the average number of revertant colonies compared to that of negative control with regard to the Ames test. Conclusions: It can be concluded that nano-HA-SiO2-GIC is non-mutagenic based on the Ames test and did not cause DNA damage at the lowest concentration of IC10 based on the Comet assay.http://www.sciencedirect.com/science/article/pii/S1013905224002384Cell viabilityDNA damageGenotoxicityHuman periodontal ligament fibroblastMutagenicityNano-hydroxyapatite-silica-glass ionomer cement |
| spellingShingle | Fayez Hussain Niazi Norhayati Luddin Abdurahman Niazy Suharni Mohamad Masitah Hayati Harun Mohammed Noushad Kannan Thirumulu Ponnuraj Mutagenicity and DNA damage assessment of locally produced nano-hydroxyapatite-silica-glass ionomer cement on human periodontal ligament fibroblasts using Ames and Comet assays Saudi Dental Journal Cell viability DNA damage Genotoxicity Human periodontal ligament fibroblast Mutagenicity Nano-hydroxyapatite-silica-glass ionomer cement |
| title | Mutagenicity and DNA damage assessment of locally produced nano-hydroxyapatite-silica-glass ionomer cement on human periodontal ligament fibroblasts using Ames and Comet assays |
| title_full | Mutagenicity and DNA damage assessment of locally produced nano-hydroxyapatite-silica-glass ionomer cement on human periodontal ligament fibroblasts using Ames and Comet assays |
| title_fullStr | Mutagenicity and DNA damage assessment of locally produced nano-hydroxyapatite-silica-glass ionomer cement on human periodontal ligament fibroblasts using Ames and Comet assays |
| title_full_unstemmed | Mutagenicity and DNA damage assessment of locally produced nano-hydroxyapatite-silica-glass ionomer cement on human periodontal ligament fibroblasts using Ames and Comet assays |
| title_short | Mutagenicity and DNA damage assessment of locally produced nano-hydroxyapatite-silica-glass ionomer cement on human periodontal ligament fibroblasts using Ames and Comet assays |
| title_sort | mutagenicity and dna damage assessment of locally produced nano hydroxyapatite silica glass ionomer cement on human periodontal ligament fibroblasts using ames and comet assays |
| topic | Cell viability DNA damage Genotoxicity Human periodontal ligament fibroblast Mutagenicity Nano-hydroxyapatite-silica-glass ionomer cement |
| url | http://www.sciencedirect.com/science/article/pii/S1013905224002384 |
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