Modulation of insulin secretion and lipid profiles through glutamate dehydrogenase activators in diabetic rabbits

Diabetes mellitus is a prevalent metabolic disorder characterized by impaired insulin secretion and aberrant lipid metabolism. Targeting glutamate dehydrogenase (GDH) activators has emerged as a potential therapeutic strategy in managing diabetes. This study aims to investigate the effects of GDH ac...

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Main Authors: Abdreshov Serik Nauryzbaevish, Galiya Tatarinova, Oxikbayev Berikzhan, Amantai Kunakbayev4, Gulnara Tashenova, Atanbaeva Gulshat Kapalbaevna, Kulbayeva Marzhan Susarovnalbayeva, Shynybekova Sholpan
Format: Article
Language:English
Published: University of Guilan 2023-12-01
Series:Caspian Journal of Environmental Sciences
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Online Access:https://cjes.guilan.ac.ir/article_7415_d957a8fba5243c22415e06b3ddb4e1dc.pdf
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author Abdreshov Serik Nauryzbaevish
Galiya Tatarinova
Oxikbayev Berikzhan
Amantai Kunakbayev4
Gulnara Tashenova
Atanbaeva Gulshat Kapalbaevna
Kulbayeva Marzhan Susarovnalbayeva
Shynybekova Sholpan
author_facet Abdreshov Serik Nauryzbaevish
Galiya Tatarinova
Oxikbayev Berikzhan
Amantai Kunakbayev4
Gulnara Tashenova
Atanbaeva Gulshat Kapalbaevna
Kulbayeva Marzhan Susarovnalbayeva
Shynybekova Sholpan
author_sort Abdreshov Serik Nauryzbaevish
collection DOAJ
description Diabetes mellitus is a prevalent metabolic disorder characterized by impaired insulin secretion and aberrant lipid metabolism. Targeting glutamate dehydrogenase (GDH) activators has emerged as a potential therapeutic strategy in managing diabetes. This study aims to investigate the effects of GDH activators on insulin secretion and lipid profiles in diabetic rabbits. Utilizing Streptozotocin (STZ) to induce diabetes in male New Zealand White rabbits, the impacts of three different GDH activators—Metformin, Epigallocatechin Gallate (EGCG), and Leucine—were examined. The subjects were categorized into five groups, including a diabetic control, a sham group, and three treatment groups administered with Metformin (5 mg kg-1), EGCG (15 mg kg-1), and Leucine (15 mg kg-1), respectively. The study reveals significant modulations in insulin and lipid profiles due to these treatments. In the Metformin-treated group, blood glucose levels significantly decreased during the second (p < 0.001) and third (p<0.01) weeks. The EGCG group exhibited a significant increase in insulin levels (p < 0.001), but no notable change in blood glucose. Conversely, the Leucine group showed an increase in triglyceride levels (p < 0.05) and a significant decrease in blood glucose levels (p < 0.01). Additionally, Metformin led to a substantial reduction in triglycerides (p < 0.001), while EGCG and Leucine were effective in lowering LDL levels (p < 0.01). Cholesterol and HDL levels remained relatively unchanged across all groups. These findings suggest that GDH activators, i.e., Metformin, EGCG, and Leucine, significantly impact insulin secretion and lipid metabolism, offering novel insights into diabetes management. This study not only demonstrates the therapeutic potential of these agents, but also emphasizes the importance of GDH pathways in diabetes research, providing a foundation for future investigations into metabolic regulation and treatment.
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1735-3866
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series Caspian Journal of Environmental Sciences
spelling doaj-art-d4fd41aeab174979976a8043e6801e2c2025-08-20T01:57:05ZengUniversity of GuilanCaspian Journal of Environmental Sciences1735-30331735-38662023-12-012151229123710.22124/cjes.2023.74157415Modulation of insulin secretion and lipid profiles through glutamate dehydrogenase activators in diabetic rabbitsAbdreshov Serik Nauryzbaevish0Galiya Tatarinova1Oxikbayev Berikzhan2Amantai Kunakbayev43Gulnara Tashenova4Atanbaeva Gulshat Kapalbaevna5Kulbayeva Marzhan Susarovnalbayeva6Shynybekova Sholpan7Institute of Genetics and Physiology CS MSHE RK, Almaty, Kazakhstan & Al-Farabi Kazakh National University, Almaty, KazakhstanInstitute of Natural Sciences and Geography, Abai Kazakh National pedagogical university, Almaty, Kazakhstan & 13, Dostyk Av., 050010 Almaty, the Republic of KazakhstanFaculty of Natural Sciences, Zhetysu University named after I. Zhansugurov Republic of KazakhstanDepartment of Anatomy with Physiology Courses, Kazakh-Russian Medical University, Almaty, Kazakhstan 13, Torekulova St., 050004 Almaty, the Republic of KazakhstanAbai Kazakh National Pedagogical University named after Abai. Department of Biology, 050010, Republic of Kazakhstan, KazakhstanAl-Farabi Kazakh National University, Almaty, KazakhstanAl-Farabi Kazakh National University, Almaty, KazakhstanInstitute of Natural Sciences and Geography of the Kazakh National Pedagogical University named after Abai, Almaty, Kazakhstan; Republic of Kazakhstan, KazakhstanDiabetes mellitus is a prevalent metabolic disorder characterized by impaired insulin secretion and aberrant lipid metabolism. Targeting glutamate dehydrogenase (GDH) activators has emerged as a potential therapeutic strategy in managing diabetes. This study aims to investigate the effects of GDH activators on insulin secretion and lipid profiles in diabetic rabbits. Utilizing Streptozotocin (STZ) to induce diabetes in male New Zealand White rabbits, the impacts of three different GDH activators—Metformin, Epigallocatechin Gallate (EGCG), and Leucine—were examined. The subjects were categorized into five groups, including a diabetic control, a sham group, and three treatment groups administered with Metformin (5 mg kg-1), EGCG (15 mg kg-1), and Leucine (15 mg kg-1), respectively. The study reveals significant modulations in insulin and lipid profiles due to these treatments. In the Metformin-treated group, blood glucose levels significantly decreased during the second (p < 0.001) and third (p<0.01) weeks. The EGCG group exhibited a significant increase in insulin levels (p < 0.001), but no notable change in blood glucose. Conversely, the Leucine group showed an increase in triglyceride levels (p < 0.05) and a significant decrease in blood glucose levels (p < 0.01). Additionally, Metformin led to a substantial reduction in triglycerides (p < 0.001), while EGCG and Leucine were effective in lowering LDL levels (p < 0.01). Cholesterol and HDL levels remained relatively unchanged across all groups. These findings suggest that GDH activators, i.e., Metformin, EGCG, and Leucine, significantly impact insulin secretion and lipid metabolism, offering novel insights into diabetes management. This study not only demonstrates the therapeutic potential of these agents, but also emphasizes the importance of GDH pathways in diabetes research, providing a foundation for future investigations into metabolic regulation and treatment.https://cjes.guilan.ac.ir/article_7415_d957a8fba5243c22415e06b3ddb4e1dc.pdfamino acidsenzymeshormonesdiabetes
spellingShingle Abdreshov Serik Nauryzbaevish
Galiya Tatarinova
Oxikbayev Berikzhan
Amantai Kunakbayev4
Gulnara Tashenova
Atanbaeva Gulshat Kapalbaevna
Kulbayeva Marzhan Susarovnalbayeva
Shynybekova Sholpan
Modulation of insulin secretion and lipid profiles through glutamate dehydrogenase activators in diabetic rabbits
Caspian Journal of Environmental Sciences
amino acids
enzymes
hormones
diabetes
title Modulation of insulin secretion and lipid profiles through glutamate dehydrogenase activators in diabetic rabbits
title_full Modulation of insulin secretion and lipid profiles through glutamate dehydrogenase activators in diabetic rabbits
title_fullStr Modulation of insulin secretion and lipid profiles through glutamate dehydrogenase activators in diabetic rabbits
title_full_unstemmed Modulation of insulin secretion and lipid profiles through glutamate dehydrogenase activators in diabetic rabbits
title_short Modulation of insulin secretion and lipid profiles through glutamate dehydrogenase activators in diabetic rabbits
title_sort modulation of insulin secretion and lipid profiles through glutamate dehydrogenase activators in diabetic rabbits
topic amino acids
enzymes
hormones
diabetes
url https://cjes.guilan.ac.ir/article_7415_d957a8fba5243c22415e06b3ddb4e1dc.pdf
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