Development of a novel strategy to reduce diagnostic errors in real-time polymerase chain reaction using probe-based techniques

Abstract Real-time PCR assays are valuable tools for the rapid and accurate diagnosis of infectious diseases by identifying the nucleic acid sequences of pathogens. Here, we aimed to develop a recombinant plasmid-based standard for validating the sensitivity of different molecular diagnostic methods...

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Bibliographic Details
Main Authors:	Hyoung Jun Kim, Morten Schiøtt, Niels Jørgen Olesen, Euna Choi, Bok Kyung Ku, Kyoung Ki Lee, Hye Young Jeong, Ilseob Lee, Seong Mok Kim, Miyoung Cho, Young Chul Kim
Format:	Article
Language:	English
Published:	Nature Portfolio 2024-11-01
Series:	Scientific Reports
Subjects:	Viral hemorrhagic septicemia Standardization Real-time PCR False-positive False-negative Positive control
Online Access:	https://doi.org/10.1038/s41598-024-78654-2
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Development of a novel strategy to reduce diagnostic errors in real-time polymerase chain reaction using probe-based techniques

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