Evaluation of the nutrient digestibility at each age in dogs diet by in vitro and in vivo methods

The objective of this study was to evaluate in vitro predictions of digestibility at each age (puppy, adult, and senior) in dogs of dry matter (DM), organic matter (OM), crude protein (CP), gross energy (GE), crude fiber (CF), and ether extract (EE) using dog diets. First, to determine the digestibi...

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Main Authors: Kyeongho Jeon, Jihwan Lee, Minho Song, Kihyun Kim, Minseok Jo, Seyeon Chang, Dongcheol Song, Sehyun Park, Hyuck Kim, Hyeun Bum Kim, Jinho Cho
Format: Article
Language:English
Published: Korean Society of Animal Sciences and Technology 2024-11-01
Series:Journal of Animal Science and Technology
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Online Access:http://www.ejast.org/archive/view_article?doi=10.5187/jast.2024.e69
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author Kyeongho Jeon
Jihwan Lee
Minho Song
Kihyun Kim
Minseok Jo
Seyeon Chang
Dongcheol Song
Sehyun Park
Hyuck Kim
Hyeun Bum Kim
Jinho Cho
author_facet Kyeongho Jeon
Jihwan Lee
Minho Song
Kihyun Kim
Minseok Jo
Seyeon Chang
Dongcheol Song
Sehyun Park
Hyuck Kim
Hyeun Bum Kim
Jinho Cho
author_sort Kyeongho Jeon
collection DOAJ
description The objective of this study was to evaluate in vitro predictions of digestibility at each age (puppy, adult, and senior) in dogs of dry matter (DM), organic matter (OM), crude protein (CP), gross energy (GE), crude fiber (CF), and ether extract (EE) using dog diets. First, to determine the digestibility of dog diets using pepsin and pancreatin incubations, conduct the in vitro method. Later, 18 mixed-sex beagles were used in this experiment to compare in vivo digestibility. Beagles are divided into 3 groups according to their age and body weight: six puppies (under 1-year-old; 6.21 ± 0.56 kg), six adult dogs (2 to 7 years old; 8.16 ± 0.64 kg), and six senior dogs (over 8 years old; 6.95 ± 1.39 kg). Except for DM in puppies and adult dogs, in all cases, in vitro digestibility values were higher than in vivo digestibility values (p < 0.05). In puppies, there were strong relationships for DM and GE with r2 values of 0.95 and 0.84, respectively, between in vitro and in vivo digestibility. Also, in adult dogs, there were strong relationships for DM and GE with r2 values of 0.97 and 0.84, respectively, between in vitro and in vivo digestibility. However, in senior dogs, there was a lower relationship for DM, OM, CP, GE, CF, and EE with r2 values of 0.18, 0.42, 0.01, 0.02, 0.11, and 0.04, respectively, between in vitro and in vivo digestibility. In conclusion, in vitro, the prediction of nutrient digestibility of DM and GE in puppies and adult dogs seems to have significant potential for practical application. However, additional research is needed to compare senior dogs with the in vitro method.
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2055-0391
language English
publishDate 2024-11-01
publisher Korean Society of Animal Sciences and Technology
record_format Article
series Journal of Animal Science and Technology
spelling doaj-art-d4f011b4b3be4602aaa9500206de0e842024-12-12T08:19:54ZengKorean Society of Animal Sciences and TechnologyJournal of Animal Science and Technology2672-01912055-03912024-11-016661273128110.5187/jast.2024.e69Evaluation of the nutrient digestibility at each age in dogs diet by in vitro and in vivo methodsKyeongho Jeon0Jihwan Lee1Minho Song2Kihyun Kim3Minseok Jo4Seyeon Chang5Dongcheol Song6Sehyun Park7Hyuck Kim8Hyeun Bum Kim9Jinho Cho10Department of Animal Science, Chungbuk National University, Cheongju 28644, KoreaSwine Science Division, National Institute of Animal Science, Rural Development Administration, Cheonan 31000, KoreaDivision of Animal and Dairy Science, Chungnam National University, Daejeon 34134, KoreaNational Institute of Animal Science, Rural Development Administration, Wanju 55365, KoreaCentral Research Institute, Woosung Feed Co., Ltd, Daejeon 34379, KoreaDepartment of Animal Science, Chungbuk National University, Cheongju 28644, KoreaDepartment of Animal Science, Chungbuk National University, Cheongju 28644, KoreaDepartment of Animal Science, Chungbuk National University, Cheongju 28644, KoreaDepartment of Animal Science, Chungbuk National University, Cheongju 28644, KoreaDepartment of Animal Biotechnology, Dankook University, Cheonan 31116, KoreaDepartment of Animal Science, Chungbuk National University, Cheongju 28644, KoreaThe objective of this study was to evaluate in vitro predictions of digestibility at each age (puppy, adult, and senior) in dogs of dry matter (DM), organic matter (OM), crude protein (CP), gross energy (GE), crude fiber (CF), and ether extract (EE) using dog diets. First, to determine the digestibility of dog diets using pepsin and pancreatin incubations, conduct the in vitro method. Later, 18 mixed-sex beagles were used in this experiment to compare in vivo digestibility. Beagles are divided into 3 groups according to their age and body weight: six puppies (under 1-year-old; 6.21 ± 0.56 kg), six adult dogs (2 to 7 years old; 8.16 ± 0.64 kg), and six senior dogs (over 8 years old; 6.95 ± 1.39 kg). Except for DM in puppies and adult dogs, in all cases, in vitro digestibility values were higher than in vivo digestibility values (p < 0.05). In puppies, there were strong relationships for DM and GE with r2 values of 0.95 and 0.84, respectively, between in vitro and in vivo digestibility. Also, in adult dogs, there were strong relationships for DM and GE with r2 values of 0.97 and 0.84, respectively, between in vitro and in vivo digestibility. However, in senior dogs, there was a lower relationship for DM, OM, CP, GE, CF, and EE with r2 values of 0.18, 0.42, 0.01, 0.02, 0.11, and 0.04, respectively, between in vitro and in vivo digestibility. In conclusion, in vitro, the prediction of nutrient digestibility of DM and GE in puppies and adult dogs seems to have significant potential for practical application. However, additional research is needed to compare senior dogs with the in vitro method. http://www.ejast.org/archive/view_article?doi=10.5187/jast.2024.e69In vitro digestibilityIn vivo digestibilityDogAge
spellingShingle Kyeongho Jeon
Jihwan Lee
Minho Song
Kihyun Kim
Minseok Jo
Seyeon Chang
Dongcheol Song
Sehyun Park
Hyuck Kim
Hyeun Bum Kim
Jinho Cho
Evaluation of the nutrient digestibility at each age in dogs diet by in vitro and in vivo methods
Journal of Animal Science and Technology
In vitro digestibility
In vivo digestibility
Dog
Age
title Evaluation of the nutrient digestibility at each age in dogs diet by in vitro and in vivo methods
title_full Evaluation of the nutrient digestibility at each age in dogs diet by in vitro and in vivo methods
title_fullStr Evaluation of the nutrient digestibility at each age in dogs diet by in vitro and in vivo methods
title_full_unstemmed Evaluation of the nutrient digestibility at each age in dogs diet by in vitro and in vivo methods
title_short Evaluation of the nutrient digestibility at each age in dogs diet by in vitro and in vivo methods
title_sort evaluation of the nutrient digestibility at each age in dogs diet by in vitro and in vivo methods
topic In vitro digestibility
In vivo digestibility
Dog
Age
url http://www.ejast.org/archive/view_article?doi=10.5187/jast.2024.e69
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