M icroRNA-27a gene polymorphism (rs895819) association with risk of pulmonary tuberculosis (PTB): A case–control study
Abstract Background Pulmonary tuberculosis (PTB), caused by the bacteria Mycobacterium tuberculosis (M.tb), remains a significant global health challenge. Research suggests that genetic factors, particularly single-nucleotide polymorphisms (SNPs) in microRNA (miRNA) genes, may influence an individua...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2025-07-01
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| Series: | Egyptian Journal of Medical Human Genetics |
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| Online Access: | https://doi.org/10.1186/s43042-025-00750-3 |
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| author | Alireza Arabshahi Mahboobeh Sabeti Akbar-Abad Ebrahim Alijani Mahdi Majidpour Sepehr Ghasemi Zahra Mohammadghasemipour Shahriyar Nikuei Razieh Akhtar Hossein Shahriari |
| author_facet | Alireza Arabshahi Mahboobeh Sabeti Akbar-Abad Ebrahim Alijani Mahdi Majidpour Sepehr Ghasemi Zahra Mohammadghasemipour Shahriyar Nikuei Razieh Akhtar Hossein Shahriari |
| author_sort | Alireza Arabshahi |
| collection | DOAJ |
| description | Abstract Background Pulmonary tuberculosis (PTB), caused by the bacteria Mycobacterium tuberculosis (M.tb), remains a significant global health challenge. Research suggests that genetic factors, particularly single-nucleotide polymorphisms (SNPs) in microRNA (miRNA) genes, may influence an individual’s susceptibility to PTB. Our analysis identified a significant correlation between the rs895819 polymorphism and an elevated risk of developing PTB. Methods and materials We designed case–control research with 60 PTB patients and 60 healthy controls matched for age and sex. We used PCR–RFLP analysis to identify the differences between the samples. We used odds ratios and multivariate logistic regression. We used it in the study to make sure that it had a good mix of healthy people who were free of active tuberculosis based on clinical testing and chest X-rays and people who had been diagnosed with PTB based on clinical and radiological findings. Results Significant results were seen in the codominant 2 model (p = 0.004), the dominant model (p = 0.013), the recessive model (p = 0.008), and the allelic models (p = 0.001), with respective risk increases for PTB of 5.15, 2.62, 4.21, and 2.71 times. Conclusions We found a strong link between the miR-27a gene polymorphism (rs895819) and a higher risk of PTB. This link shows how important genetic factors are in determining the risk of getting PTB and how genetic screening could be used in public health programs to find people who are at risk. Researchers should look into how miR-27a affects the immune system’s response to tuberculosis in the future and think about what this means for therapeutic interventions. |
| format | Article |
| id | doaj-art-d4ecb5c55b6940abaf9f6722b3e87478 |
| institution | DOAJ |
| issn | 2090-2441 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Medical Human Genetics |
| spelling | doaj-art-d4ecb5c55b6940abaf9f6722b3e874782025-08-20T03:05:09ZengSpringerOpenEgyptian Journal of Medical Human Genetics2090-24412025-07-0126111010.1186/s43042-025-00750-3M icroRNA-27a gene polymorphism (rs895819) association with risk of pulmonary tuberculosis (PTB): A case–control studyAlireza Arabshahi0Mahboobeh Sabeti Akbar-Abad1Ebrahim Alijani2Mahdi Majidpour3Sepehr Ghasemi4Zahra Mohammadghasemipour5Shahriyar Nikuei6Razieh Akhtar7Hossein Shahriari8Clinical Immunology Research Centre, Zahedan University of Medical SciencesDepartment of Clinical Biochemistry, School of Medicine, Zahedan University of Medical SciencesClinical Immunology Research Centre, Zahedan University of Medical SciencesClinical Immunology Research Centre, Zahedan University of Medical SciencesStudent Research Committee, Zahedan University of Medical SciencesClinical Immunology Research Centre, Zahedan University of Medical SciencesStudent Research Committee, Zahedan University of Medical SciencesStudent Research Committee, Zahedan University of Medical SciencesClinical Immunology Research Centre, Zahedan University of Medical SciencesAbstract Background Pulmonary tuberculosis (PTB), caused by the bacteria Mycobacterium tuberculosis (M.tb), remains a significant global health challenge. Research suggests that genetic factors, particularly single-nucleotide polymorphisms (SNPs) in microRNA (miRNA) genes, may influence an individual’s susceptibility to PTB. Our analysis identified a significant correlation between the rs895819 polymorphism and an elevated risk of developing PTB. Methods and materials We designed case–control research with 60 PTB patients and 60 healthy controls matched for age and sex. We used PCR–RFLP analysis to identify the differences between the samples. We used odds ratios and multivariate logistic regression. We used it in the study to make sure that it had a good mix of healthy people who were free of active tuberculosis based on clinical testing and chest X-rays and people who had been diagnosed with PTB based on clinical and radiological findings. Results Significant results were seen in the codominant 2 model (p = 0.004), the dominant model (p = 0.013), the recessive model (p = 0.008), and the allelic models (p = 0.001), with respective risk increases for PTB of 5.15, 2.62, 4.21, and 2.71 times. Conclusions We found a strong link between the miR-27a gene polymorphism (rs895819) and a higher risk of PTB. This link shows how important genetic factors are in determining the risk of getting PTB and how genetic screening could be used in public health programs to find people who are at risk. Researchers should look into how miR-27a affects the immune system’s response to tuberculosis in the future and think about what this means for therapeutic interventions.https://doi.org/10.1186/s43042-025-00750-3miR-27aPTBPolymorphism |
| spellingShingle | Alireza Arabshahi Mahboobeh Sabeti Akbar-Abad Ebrahim Alijani Mahdi Majidpour Sepehr Ghasemi Zahra Mohammadghasemipour Shahriyar Nikuei Razieh Akhtar Hossein Shahriari M icroRNA-27a gene polymorphism (rs895819) association with risk of pulmonary tuberculosis (PTB): A case–control study Egyptian Journal of Medical Human Genetics miR-27a PTB Polymorphism |
| title | M icroRNA-27a gene polymorphism (rs895819) association with risk of pulmonary tuberculosis (PTB): A case–control study |
| title_full | M icroRNA-27a gene polymorphism (rs895819) association with risk of pulmonary tuberculosis (PTB): A case–control study |
| title_fullStr | M icroRNA-27a gene polymorphism (rs895819) association with risk of pulmonary tuberculosis (PTB): A case–control study |
| title_full_unstemmed | M icroRNA-27a gene polymorphism (rs895819) association with risk of pulmonary tuberculosis (PTB): A case–control study |
| title_short | M icroRNA-27a gene polymorphism (rs895819) association with risk of pulmonary tuberculosis (PTB): A case–control study |
| title_sort | m icrorna 27a gene polymorphism rs895819 association with risk of pulmonary tuberculosis ptb a case control study |
| topic | miR-27a PTB Polymorphism |
| url | https://doi.org/10.1186/s43042-025-00750-3 |
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