Genome Analysis of a Polysaccharide-Degrading Bacterium <i>Microbulbifer</i> sp. HZ11 and Degradation of Alginate
Marine bacteria are crucial sources of alginate lyases, which play an essential role in alginate oligosaccharide (AOS) production. This study reports the biochemical characteristics of a new species of the <i>Microbulbifer</i> genus, <i>Microbulbifer</i> sp. HZ11. The strain...
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2024-12-01
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| author | Xiao Liu Wentao Zhao Yan Li Zhongliang Sun Chang Lu Liqin Sun |
| author_facet | Xiao Liu Wentao Zhao Yan Li Zhongliang Sun Chang Lu Liqin Sun |
| author_sort | Xiao Liu |
| collection | DOAJ |
| description | Marine bacteria are crucial sources of alginate lyases, which play an essential role in alginate oligosaccharide (AOS) production. This study reports the biochemical characteristics of a new species of the <i>Microbulbifer</i> genus, <i>Microbulbifer</i> sp. HZ11. The strain HZ11 is Gram-negative, aerobic, flagellate-free, and rod-shaped. The genome of strain HZ11 is a 4,248,867 bp circular chromosome with an average GC content of 56.68%. HZ11 can degrade alginate and other polysaccharides. The carbohydrate-active enzyme (CAZyme) genes account for 4.57% of the total protein-coding genes of HZ11. Its alginate metabolism process is consistent with the characteristics of the polysaccharide utilization locus (PUL) system. The alginate lyase produced by strain HZ11 showed the highest activity at 50 °C, pH 8.5, and 0.1 M NaCl. The substrate preference was as follows: sodium alginate > poly mannuronic acid > poly guluronic acid. The thin layer chromatography (TLC) results revealed that the main enzymatic degradation products were monosaccharides or AOSs with a degree of polymerization (DP) of 2–3. These results help clarify the metabolism and utilization mechanism of alginate by marine bacteria and provide a theoretical reference for its application in the degradation of alginate and other polysaccharides. |
| format | Article |
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| language | English |
| publishDate | 2024-12-01 |
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| series | Marine Drugs |
| spelling | doaj-art-d4e45e0cc0c8487d9e28cac94bd15e4f2025-08-20T02:00:47ZengMDPI AGMarine Drugs1660-33972024-12-01221256910.3390/md22120569Genome Analysis of a Polysaccharide-Degrading Bacterium <i>Microbulbifer</i> sp. HZ11 and Degradation of AlginateXiao Liu0Wentao Zhao1Yan Li2Zhongliang Sun3Chang Lu4Liqin Sun5Yantai Key Laboratory of Characteristic Agricultural Bioresource Conservation & Germplasm Innovative Utilization, School of Life Sciences, Yantai University, Yantai 264000, ChinaYantai Key Laboratory of Characteristic Agricultural Bioresource Conservation & Germplasm Innovative Utilization, School of Life Sciences, Yantai University, Yantai 264000, ChinaYantai Key Laboratory of Characteristic Agricultural Bioresource Conservation & Germplasm Innovative Utilization, School of Life Sciences, Yantai University, Yantai 264000, ChinaYantai Key Laboratory of Characteristic Agricultural Bioresource Conservation & Germplasm Innovative Utilization, School of Life Sciences, Yantai University, Yantai 264000, ChinaYantai Key Laboratory of Characteristic Agricultural Bioresource Conservation & Germplasm Innovative Utilization, School of Life Sciences, Yantai University, Yantai 264000, ChinaYantai Key Laboratory of Characteristic Agricultural Bioresource Conservation & Germplasm Innovative Utilization, School of Life Sciences, Yantai University, Yantai 264000, ChinaMarine bacteria are crucial sources of alginate lyases, which play an essential role in alginate oligosaccharide (AOS) production. This study reports the biochemical characteristics of a new species of the <i>Microbulbifer</i> genus, <i>Microbulbifer</i> sp. HZ11. The strain HZ11 is Gram-negative, aerobic, flagellate-free, and rod-shaped. The genome of strain HZ11 is a 4,248,867 bp circular chromosome with an average GC content of 56.68%. HZ11 can degrade alginate and other polysaccharides. The carbohydrate-active enzyme (CAZyme) genes account for 4.57% of the total protein-coding genes of HZ11. Its alginate metabolism process is consistent with the characteristics of the polysaccharide utilization locus (PUL) system. The alginate lyase produced by strain HZ11 showed the highest activity at 50 °C, pH 8.5, and 0.1 M NaCl. The substrate preference was as follows: sodium alginate > poly mannuronic acid > poly guluronic acid. The thin layer chromatography (TLC) results revealed that the main enzymatic degradation products were monosaccharides or AOSs with a degree of polymerization (DP) of 2–3. These results help clarify the metabolism and utilization mechanism of alginate by marine bacteria and provide a theoretical reference for its application in the degradation of alginate and other polysaccharides.https://www.mdpi.com/1660-3397/22/12/569<i>Microbulbifer</i> sp.alginate lyasepolysaccharide lyasealginate-degrading strain |
| spellingShingle | Xiao Liu Wentao Zhao Yan Li Zhongliang Sun Chang Lu Liqin Sun Genome Analysis of a Polysaccharide-Degrading Bacterium <i>Microbulbifer</i> sp. HZ11 and Degradation of Alginate Marine Drugs <i>Microbulbifer</i> sp. alginate lyase polysaccharide lyase alginate-degrading strain |
| title | Genome Analysis of a Polysaccharide-Degrading Bacterium <i>Microbulbifer</i> sp. HZ11 and Degradation of Alginate |
| title_full | Genome Analysis of a Polysaccharide-Degrading Bacterium <i>Microbulbifer</i> sp. HZ11 and Degradation of Alginate |
| title_fullStr | Genome Analysis of a Polysaccharide-Degrading Bacterium <i>Microbulbifer</i> sp. HZ11 and Degradation of Alginate |
| title_full_unstemmed | Genome Analysis of a Polysaccharide-Degrading Bacterium <i>Microbulbifer</i> sp. HZ11 and Degradation of Alginate |
| title_short | Genome Analysis of a Polysaccharide-Degrading Bacterium <i>Microbulbifer</i> sp. HZ11 and Degradation of Alginate |
| title_sort | genome analysis of a polysaccharide degrading bacterium i microbulbifer i sp hz11 and degradation of alginate |
| topic | <i>Microbulbifer</i> sp. alginate lyase polysaccharide lyase alginate-degrading strain |
| url | https://www.mdpi.com/1660-3397/22/12/569 |
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