Polyclonal spread of blaOXA-23 and blaOXA-58 in Acinetobacter baumannii isolates from Argentina
Background: In order to study the enzymatic carbapenem resistance mechanisms in Acinetobacter baumannii isolates from Argentina, we performed molecular characterization on 41 epidemiologically unrelated strains isolated from 1995 to 2006 with diminished susceptibilities to imipenem and meropenem. Me...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Journal of Infection in Developing Countries
2008-06-01
|
| Series: | Journal of Infection in Developing Countries |
| Subjects: | |
| Online Access: | https://jidc.org/index.php/journal/article/view/269 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849323593988571136 |
|---|---|
| author | Andrea Karina Merkier Mariana Catalano Maria Soledad Ramirez Cecilia Quiroga Betina Orman Laura Ratier Angela Famiglietti Carlos Vay Ana Di Martino Sara Kaufman Daniela Centrón |
| author_facet | Andrea Karina Merkier Mariana Catalano Maria Soledad Ramirez Cecilia Quiroga Betina Orman Laura Ratier Angela Famiglietti Carlos Vay Ana Di Martino Sara Kaufman Daniela Centrón |
| author_sort | Andrea Karina Merkier |
| collection | DOAJ |
| description | Background: In order to study the enzymatic carbapenem resistance mechanisms in Acinetobacter baumannii isolates from Argentina, we performed molecular characterization on 41 epidemiologically unrelated strains isolated from 1995 to 2006 with diminished susceptibilities to imipenem and meropenem.
Methodology: Acinetobacter baumannii isolates were identified with the ARDRA technique. The total genomic DNA was used to detect each carbapenem β-lactamase gene described so far in this species and those insertion sequences usually associated to carbapenem β-lactamase genes (ISAba1, 2, 3, 4 and IS18) by the PCR technique with specific primers.
Results: 26 out of 41 Acinetobacter baumannii isolates with diminished susceptibilities to carbapenems harboured the blaOXA-23 gene. The blaOXA-58 was detected in 13 out of 41 isolates. ISAba1 was always located upstream blaOXA-23. All isolates containing the blaOXA-58 gene showed ISAba3 downstream of the carbapenemase, while 4 isolates had a second copy of the ISAba3 upstream of the gene.
Conclusion: Enzymatic carbapenem resistance in Acinetobacter baumannii was found in 88% of 41 non-epidemiologically-related strains mediated by the polyclonal spread of the blaOXA-23 and blaOXA-58 genes. The genetic structures surrounding the oxacillinase genes found in our bacterial isolates revealed a particular epidemiology in our geographical region. This data suggests the need of local molecular surveillance to help control multirresistance Acinetobacter baumannii infections. |
| format | Article |
| id | doaj-art-d4e38eea07fe4922ba4412036e1dc1f2 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2008-06-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-d4e38eea07fe4922ba4412036e1dc1f22025-08-20T03:48:58ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802008-06-0120310.3855/jidc.269Polyclonal spread of blaOXA-23 and blaOXA-58 in Acinetobacter baumannii isolates from ArgentinaAndrea Karina Merkier0Mariana Catalano1Maria Soledad Ramirez2Cecilia Quiroga3Betina Orman4Laura Ratier5Angela Famiglietti6Carlos Vay7Ana Di Martino8Sara Kaufman9Daniela Centrón10Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155. Buenos AiresDepartamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155. Buenos AiresDepartamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155. Buenos AiresDepartamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155. Buenos AiresDepartamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155. Buenos AiresDepartamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155. Buenos AiresLaboratorio de Bacteriología, Departamento de Análisis Clínicos, Hospital de Clínicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 954. Buenos AiresLaboratorio de Bacteriología, Departamento de Análisis Clínicos, Hospital de Clínicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 954. Buenos AiresServicio de Infectología, Sanatorio Mitre, Bartolomé Mitre 365. Buenos AiresHospital Juan Fernández, Cerviño 3356. Buenos AiresDepartamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155. Buenos AiresBackground: In order to study the enzymatic carbapenem resistance mechanisms in Acinetobacter baumannii isolates from Argentina, we performed molecular characterization on 41 epidemiologically unrelated strains isolated from 1995 to 2006 with diminished susceptibilities to imipenem and meropenem. Methodology: Acinetobacter baumannii isolates were identified with the ARDRA technique. The total genomic DNA was used to detect each carbapenem β-lactamase gene described so far in this species and those insertion sequences usually associated to carbapenem β-lactamase genes (ISAba1, 2, 3, 4 and IS18) by the PCR technique with specific primers. Results: 26 out of 41 Acinetobacter baumannii isolates with diminished susceptibilities to carbapenems harboured the blaOXA-23 gene. The blaOXA-58 was detected in 13 out of 41 isolates. ISAba1 was always located upstream blaOXA-23. All isolates containing the blaOXA-58 gene showed ISAba3 downstream of the carbapenemase, while 4 isolates had a second copy of the ISAba3 upstream of the gene. Conclusion: Enzymatic carbapenem resistance in Acinetobacter baumannii was found in 88% of 41 non-epidemiologically-related strains mediated by the polyclonal spread of the blaOXA-23 and blaOXA-58 genes. The genetic structures surrounding the oxacillinase genes found in our bacterial isolates revealed a particular epidemiology in our geographical region. This data suggests the need of local molecular surveillance to help control multirresistance Acinetobacter baumannii infections.https://jidc.org/index.php/journal/article/view/269Acinetobacter baumannicarbapenemasesinsertion sequence |
| spellingShingle | Andrea Karina Merkier Mariana Catalano Maria Soledad Ramirez Cecilia Quiroga Betina Orman Laura Ratier Angela Famiglietti Carlos Vay Ana Di Martino Sara Kaufman Daniela Centrón Polyclonal spread of blaOXA-23 and blaOXA-58 in Acinetobacter baumannii isolates from Argentina Journal of Infection in Developing Countries Acinetobacter baumanni carbapenemases insertion sequence |
| title | Polyclonal spread of blaOXA-23 and blaOXA-58 in Acinetobacter baumannii isolates from Argentina |
| title_full | Polyclonal spread of blaOXA-23 and blaOXA-58 in Acinetobacter baumannii isolates from Argentina |
| title_fullStr | Polyclonal spread of blaOXA-23 and blaOXA-58 in Acinetobacter baumannii isolates from Argentina |
| title_full_unstemmed | Polyclonal spread of blaOXA-23 and blaOXA-58 in Acinetobacter baumannii isolates from Argentina |
| title_short | Polyclonal spread of blaOXA-23 and blaOXA-58 in Acinetobacter baumannii isolates from Argentina |
| title_sort | polyclonal spread of blaoxa 23 and blaoxa 58 in acinetobacter baumannii isolates from argentina |
| topic | Acinetobacter baumanni carbapenemases insertion sequence |
| url | https://jidc.org/index.php/journal/article/view/269 |
| work_keys_str_mv | AT andreakarinamerkier polyclonalspreadofblaoxa23andblaoxa58inacinetobacterbaumanniiisolatesfromargentina AT marianacatalano polyclonalspreadofblaoxa23andblaoxa58inacinetobacterbaumanniiisolatesfromargentina AT mariasoledadramirez polyclonalspreadofblaoxa23andblaoxa58inacinetobacterbaumanniiisolatesfromargentina AT ceciliaquiroga polyclonalspreadofblaoxa23andblaoxa58inacinetobacterbaumanniiisolatesfromargentina AT betinaorman polyclonalspreadofblaoxa23andblaoxa58inacinetobacterbaumanniiisolatesfromargentina AT lauraratier polyclonalspreadofblaoxa23andblaoxa58inacinetobacterbaumanniiisolatesfromargentina AT angelafamiglietti polyclonalspreadofblaoxa23andblaoxa58inacinetobacterbaumanniiisolatesfromargentina AT carlosvay polyclonalspreadofblaoxa23andblaoxa58inacinetobacterbaumanniiisolatesfromargentina AT anadimartino polyclonalspreadofblaoxa23andblaoxa58inacinetobacterbaumanniiisolatesfromargentina AT sarakaufman polyclonalspreadofblaoxa23andblaoxa58inacinetobacterbaumanniiisolatesfromargentina AT danielacentron polyclonalspreadofblaoxa23andblaoxa58inacinetobacterbaumanniiisolatesfromargentina |