Downregulation of microRNA-181d had suppressive effect on pancreatic cancer development through inverse regulation of KNAIN2
We explored the expression and function of miR-181d (microRNA-181d) in human pancreatic cancer. Quantitative real-time polymerase chain reaction was used to probe miR-181d expression in both pancreatic cancer cell lines and human pancreatic carcinoma. Pancreatic cancer cell lines, PANC-1 and AsPC-1...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2017-04-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317698364 |
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| author | Guopeng Zhang Dongbo Liu Guoxian Long Lei Shi Hong Qiu Guangyuan Hu Guoqing Hu Shunfang Liu |
| author_facet | Guopeng Zhang Dongbo Liu Guoxian Long Lei Shi Hong Qiu Guangyuan Hu Guoqing Hu Shunfang Liu |
| author_sort | Guopeng Zhang |
| collection | DOAJ |
| description | We explored the expression and function of miR-181d (microRNA-181d) in human pancreatic cancer. Quantitative real-time polymerase chain reaction was used to probe miR-181d expression in both pancreatic cancer cell lines and human pancreatic carcinoma. Pancreatic cancer cell lines, PANC-1 and AsPC-1 cells, were engineered to stably downregulate endogenous miR-181d through lentiviral transduction. The mechanistic effects of miR-181d downregulation on pancreatic cancer development were tested by proliferation, migration, fluorouracil chemosensitivity assays in vitro, and explant assay in vivo. Possible miR-181d downstream gene, NKAIN2 (Na+/K+ transporting ATPase interacting 2), was tested by dual-luciferase activity assay and quantitative real-time polymerase chain reaction. Functional involvement of NKAIN2 in miR-181d-regulated pancreatic cancer development was tested by small interfering RNA–mediated NKAIN2 knockdown in miR-181d-downregulated PANC-1 and AsPC-1 cells. MiR-181d was upregulated in both pancreatic cancer cell lines and human pancreatic carcinoma. Lentivirus-induced miR-181d downregulation decreased pancreatic cancer proliferation, migration, and fluorouracil resistance in vitro and inhibited the growth of cancer explant in vivo. NKAIN2 was directly targeted by miR-181d in pancreatic cancer. Small interfering RNA–mediated NKAIN2 knockdown reversed the inhibition of miR-181d downregulation on pancreatic cancer development. MiR-181d is aberrantly overexpressed in pancreatic cancer. Inhibiting miR-181d may suppress pancreatic cancer development, possibly through the inverse regulation on NKAIN2. |
| format | Article |
| id | doaj-art-d4dd3ce5cd1a4fbbb8326e24c017b4fa |
| institution | Kabale University |
| issn | 1423-0380 |
| language | English |
| publishDate | 2017-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-d4dd3ce5cd1a4fbbb8326e24c017b4fa2025-08-20T03:57:43ZengSAGE PublishingTumor Biology1423-03802017-04-013910.1177/1010428317698364Downregulation of microRNA-181d had suppressive effect on pancreatic cancer development through inverse regulation of KNAIN2Guopeng Zhang0Dongbo Liu1Guoxian Long2Lei Shi3Hong Qiu4Guangyuan Hu5Guoqing Hu6Shunfang Liu7Department of Nuclear Medicine, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Oncology, Affiliated Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Oncology, Affiliated Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Oncology, Affiliated Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Oncology, Affiliated Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Oncology, Affiliated Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Oncology, Affiliated Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Oncology, Affiliated Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaWe explored the expression and function of miR-181d (microRNA-181d) in human pancreatic cancer. Quantitative real-time polymerase chain reaction was used to probe miR-181d expression in both pancreatic cancer cell lines and human pancreatic carcinoma. Pancreatic cancer cell lines, PANC-1 and AsPC-1 cells, were engineered to stably downregulate endogenous miR-181d through lentiviral transduction. The mechanistic effects of miR-181d downregulation on pancreatic cancer development were tested by proliferation, migration, fluorouracil chemosensitivity assays in vitro, and explant assay in vivo. Possible miR-181d downstream gene, NKAIN2 (Na+/K+ transporting ATPase interacting 2), was tested by dual-luciferase activity assay and quantitative real-time polymerase chain reaction. Functional involvement of NKAIN2 in miR-181d-regulated pancreatic cancer development was tested by small interfering RNA–mediated NKAIN2 knockdown in miR-181d-downregulated PANC-1 and AsPC-1 cells. MiR-181d was upregulated in both pancreatic cancer cell lines and human pancreatic carcinoma. Lentivirus-induced miR-181d downregulation decreased pancreatic cancer proliferation, migration, and fluorouracil resistance in vitro and inhibited the growth of cancer explant in vivo. NKAIN2 was directly targeted by miR-181d in pancreatic cancer. Small interfering RNA–mediated NKAIN2 knockdown reversed the inhibition of miR-181d downregulation on pancreatic cancer development. MiR-181d is aberrantly overexpressed in pancreatic cancer. Inhibiting miR-181d may suppress pancreatic cancer development, possibly through the inverse regulation on NKAIN2.https://doi.org/10.1177/1010428317698364 |
| spellingShingle | Guopeng Zhang Dongbo Liu Guoxian Long Lei Shi Hong Qiu Guangyuan Hu Guoqing Hu Shunfang Liu Downregulation of microRNA-181d had suppressive effect on pancreatic cancer development through inverse regulation of KNAIN2 Tumor Biology |
| title | Downregulation of microRNA-181d had suppressive effect on pancreatic cancer development through inverse regulation of KNAIN2 |
| title_full | Downregulation of microRNA-181d had suppressive effect on pancreatic cancer development through inverse regulation of KNAIN2 |
| title_fullStr | Downregulation of microRNA-181d had suppressive effect on pancreatic cancer development through inverse regulation of KNAIN2 |
| title_full_unstemmed | Downregulation of microRNA-181d had suppressive effect on pancreatic cancer development through inverse regulation of KNAIN2 |
| title_short | Downregulation of microRNA-181d had suppressive effect on pancreatic cancer development through inverse regulation of KNAIN2 |
| title_sort | downregulation of microrna 181d had suppressive effect on pancreatic cancer development through inverse regulation of knain2 |
| url | https://doi.org/10.1177/1010428317698364 |
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