Immunomodulatory Effects of the Tobacco Defensin NaD1
<b>Background/Objectives:</b> Defensins are important components of the innate plant immune system, exhibiting antimicrobial activity against phytopathogens, as well as against fungi pathogenic to humans. Along with antifungal activity, plant defensins are also capable of influencing var...
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MDPI AG
2024-11-01
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| author | Ekaterina I. Finkina Ivan V. Bogdanov Olga V. Shevchenko Serafima I. Fateeva Anastasia A. Ignatova Sergey V. Balandin Tatiana V. Ovchinnikova |
| author_facet | Ekaterina I. Finkina Ivan V. Bogdanov Olga V. Shevchenko Serafima I. Fateeva Anastasia A. Ignatova Sergey V. Balandin Tatiana V. Ovchinnikova |
| author_sort | Ekaterina I. Finkina |
| collection | DOAJ |
| description | <b>Background/Objectives:</b> Defensins are important components of the innate plant immune system, exhibiting antimicrobial activity against phytopathogens, as well as against fungi pathogenic to humans. Along with antifungal activity, plant defensins are also capable of influencing various immune processes, but not much is known about these effects. In this study, we investigated the immunomodulatory effects of the tobacco defensin NaD1, which possesses a pronounced antifungal activity. <b>Methods and Results:</b> We showed that NaD1 could penetrate the Caco-2 polarized monolayer. Using a multiplex assay with a panel of 48 cytokines, chemokines and growth factors, we demonstrated that NaD1 at a concentration of 2 μM had immunomodulatory effects on human dendritic cells and blood monocytes, mainly inhibiting the production of various immune factors. Using the sandwich ELISA method, we demonstrated that NaD1 at the same concentration had a pronounced immunomodulatory effect on unstimulated THP-1-derived macrophages and those stimulated by bacterial LPS or fungal zymosan. NaD1 had a dual effect and induced the production of both pro-inflammatory cytokine IL-1β as well as anti-inflammatory IL-10 on resting and pro-inflammatory THP-1-derived macrophages. We also found that the immunomodulatory effects of the tobacco defensin NaD1 and the pea defensin Psd1 differed from each other, indicating nonuniformity in the modes of action of plant defensins. <b>Conclusions:</b> Thus, our data demonstrated that the tobacco defensin NaD1 exhibits different immunomodulatory effects on various immune cells. We hypothesized that influence on human immune system along with antifungal activity, could determine the effectiveness of this peptide under infection in vivo. |
| format | Article |
| id | doaj-art-d4d7733a50e54c41a1088432f77de261 |
| institution | OA Journals |
| issn | 2079-6382 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj-art-d4d7733a50e54c41a1088432f77de2612025-08-20T02:08:12ZengMDPI AGAntibiotics2079-63822024-11-011311110110.3390/antibiotics13111101Immunomodulatory Effects of the Tobacco Defensin NaD1Ekaterina I. Finkina0Ivan V. Bogdanov1Olga V. Shevchenko2Serafima I. Fateeva3Anastasia A. Ignatova4Sergey V. Balandin5Tatiana V. Ovchinnikova6M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaM.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaM.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaM.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaM.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaM.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, RussiaM.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia<b>Background/Objectives:</b> Defensins are important components of the innate plant immune system, exhibiting antimicrobial activity against phytopathogens, as well as against fungi pathogenic to humans. Along with antifungal activity, plant defensins are also capable of influencing various immune processes, but not much is known about these effects. In this study, we investigated the immunomodulatory effects of the tobacco defensin NaD1, which possesses a pronounced antifungal activity. <b>Methods and Results:</b> We showed that NaD1 could penetrate the Caco-2 polarized monolayer. Using a multiplex assay with a panel of 48 cytokines, chemokines and growth factors, we demonstrated that NaD1 at a concentration of 2 μM had immunomodulatory effects on human dendritic cells and blood monocytes, mainly inhibiting the production of various immune factors. Using the sandwich ELISA method, we demonstrated that NaD1 at the same concentration had a pronounced immunomodulatory effect on unstimulated THP-1-derived macrophages and those stimulated by bacterial LPS or fungal zymosan. NaD1 had a dual effect and induced the production of both pro-inflammatory cytokine IL-1β as well as anti-inflammatory IL-10 on resting and pro-inflammatory THP-1-derived macrophages. We also found that the immunomodulatory effects of the tobacco defensin NaD1 and the pea defensin Psd1 differed from each other, indicating nonuniformity in the modes of action of plant defensins. <b>Conclusions:</b> Thus, our data demonstrated that the tobacco defensin NaD1 exhibits different immunomodulatory effects on various immune cells. We hypothesized that influence on human immune system along with antifungal activity, could determine the effectiveness of this peptide under infection in vivo.https://www.mdpi.com/2079-6382/13/11/1101antimicrobial peptides (AMPs)host defense peptidesplant defensinstobacco defensin NaD1pea defensin Psd1human β-defensin 2 (HBD2) |
| spellingShingle | Ekaterina I. Finkina Ivan V. Bogdanov Olga V. Shevchenko Serafima I. Fateeva Anastasia A. Ignatova Sergey V. Balandin Tatiana V. Ovchinnikova Immunomodulatory Effects of the Tobacco Defensin NaD1 Antibiotics antimicrobial peptides (AMPs) host defense peptides plant defensins tobacco defensin NaD1 pea defensin Psd1 human β-defensin 2 (HBD2) |
| title | Immunomodulatory Effects of the Tobacco Defensin NaD1 |
| title_full | Immunomodulatory Effects of the Tobacco Defensin NaD1 |
| title_fullStr | Immunomodulatory Effects of the Tobacco Defensin NaD1 |
| title_full_unstemmed | Immunomodulatory Effects of the Tobacco Defensin NaD1 |
| title_short | Immunomodulatory Effects of the Tobacco Defensin NaD1 |
| title_sort | immunomodulatory effects of the tobacco defensin nad1 |
| topic | antimicrobial peptides (AMPs) host defense peptides plant defensins tobacco defensin NaD1 pea defensin Psd1 human β-defensin 2 (HBD2) |
| url | https://www.mdpi.com/2079-6382/13/11/1101 |
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