Short-Term Fertilization with the Nitrogen-Fixing Bacterium (NFB) <i>Kosakonia radicincitans</i> GXGL-4A Agent Can Modify the Transcriptome Expression Profiling of Cucumber (<i>Cucumis sativus</i> L.) Root
The application of nitrogen-fixing bacteria (NFB) as a biofertilizer can greatly reduce or even avoid environmental pollution caused by the excessive use of chemical nitrogen fertilizers. To explore the effect of short-term fertilization of GXGL-4A on the expression of functional genes in the roots...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
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| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/3/506 |
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| Summary: | The application of nitrogen-fixing bacteria (NFB) as a biofertilizer can greatly reduce or even avoid environmental pollution caused by the excessive use of chemical nitrogen fertilizers. To explore the effect of short-term fertilization of GXGL-4A on the expression of functional genes in the roots of the cucumber (<i>Cucumis sativus</i> L.) cultivar “Xintaimici”, this study used transcriptome sequencing technology combined with fluorescent quantitative RT-PCR (qRT-PCR) verification to compare the gene transcription profiles of GXGL-4A-treated and control (sterile-water-treated) groups. A total of 418 differentially expressed genes (DEGs) were detected. The transcription levels of genes <i>Csa5G161290</i> and <i>Csa3G027720</i>, which encode nitrate transporters, showed significant up-regulation (3.04- and 2.27-fold, respectively) in roots inoculated with GXGL-4A. The genes <i>CsaV3_5G006200</i>, encoding cytokinin dehydrogenase involved in the biosynthesis of zeatin, <i>CsaV3_1G011730</i>, encoding a wound-responsive protein, and <i>CsaV3_6G015610</i>, encoding a heat stress transcription factor, were significantly up-regulated at the transcriptional level (<i>p</i> < 0.05). However, the transcription of nitrogen cycling functional genes <i>CsaV3_3G036500</i>, <i>CsaV3_1g008910</i>, and <i>CsaV3_3G018610</i>, which encode nitrate reductase, high-affinity nitrate transporter (NRT), and ferredoxin-nitrite reductase, respectively, showed significant down-regulation (<i>p</i> < 0.05). Only the KEGG pathway of phenylpropanoid biosynthesis reached a significant level (<i>p</i> < 0.05). This study contributes to a deeper understanding of the interaction between NFB and plants and provides theoretical guidance for the development of GXGL-4A as a mature biological agent for sustainable agricultural production under drought stress. |
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| ISSN: | 2076-2607 |