<i>Erwinia plantamica</i> sp. nov., a Non-Phytopathogenic Bacterium Isolated from the Seedlings of Spring Wheat (<i>Triticum aestivum</i> L.)
<i>Erwinia</i> are widely known as phytopathogenic bacteria, but among them, there are also plant-friendly strains that can promote plant growth (PGPR). The <i>Erwinia</i>-like strain OPT-41 was isolated from <i>Triticum aestivum</i> seedlings as a potential PGPR....
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2025-02-01
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| author | Anna Egorshina Mikhail Lukyantsev Sergey Golubev Eugenia Boulygina Irina Khilyas Anna Muratova |
| author_facet | Anna Egorshina Mikhail Lukyantsev Sergey Golubev Eugenia Boulygina Irina Khilyas Anna Muratova |
| author_sort | Anna Egorshina |
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| description | <i>Erwinia</i> are widely known as phytopathogenic bacteria, but among them, there are also plant-friendly strains that can promote plant growth (PGPR). The <i>Erwinia</i>-like strain OPT-41 was isolated from <i>Triticum aestivum</i> seedlings as a potential PGPR. The cells (0.9–1.3 × 1.5–3.1 µm) of this microorganism are Gram-negative, rod-shaped, motile (with peritrichous flagella), and non-spore- and non-capsule-forming. The 16S rRNA gene sequence analyses showed it is located in the <i>Erwiniaceae</i> family and has a pairwise similarity above the species delineation threshold of 98.65% with several of its members: <i>Erwinia tasmaniensis</i> (99.21%), <i>Candidatus</i> Pantoea bathycoeliae (98.93%), <i>Pantoea agglomerans</i> (98.87%), <i>Erwinia endophytica</i> (98.83%), <i>Erwinia persicina</i> (98.82%), <i>Erwinia billingiae</i> (98.76%) and <i>Erwinia aphidicola</i> (98.75%). Whole genome-based taxonomy performed on the Type (Strain) Genome Server clarified the status of strain OPT-41, detecting it as a potential new species in the genus <i>Erwinia</i>. The microorganism under study was the most closely related to the type strain of <i>E. phyllosphaerae</i>, demonstrating 27.2% similarity in dDDH, 83.44% similarity in OrthoANIu, and 1.9% difference in G+C content. The major fatty acids of strain OPT-41 were 9 C<sub>16:1</sub>, C<sub>14:0</sub>, and C<sub>16:0</sub>. A combination of genome-based taxonomy and traditional polyphasic taxonomy clearly indicated that strain OPT-41 belongs to a novel <i>Erwinia</i> species, for which the name <i>E. plantamica</i> sp. nov was proposed. OPT-41 (=IBPPM 712=VKM B-3873D=CCTCC AB 2024361) has been designated as the type strain. In addition, OPT-41 was found to have low degradation potential for host plant pectins and proteins and be friendly in <i>Triticum aestivum</i> and <i>Hordeum vulgare</i> crops. |
| format | Article |
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| spelling | doaj-art-d4cbf6e33295404085f8c68d2d67f10f2025-08-20T02:42:22ZengMDPI AGMicroorganisms2076-26072025-02-0113347410.3390/microorganisms13030474<i>Erwinia plantamica</i> sp. nov., a Non-Phytopathogenic Bacterium Isolated from the Seedlings of Spring Wheat (<i>Triticum aestivum</i> L.)Anna Egorshina0Mikhail Lukyantsev1Sergey Golubev2Eugenia Boulygina3Irina Khilyas4Anna Muratova5Research and Development, Organic Park LLC, 420095 Kazan, RussiaResearch and Development, Organic Park LLC, 420095 Kazan, RussiaInstitute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), 410049 Saratov, RussiaInstitute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420021 Kazan, RussiaInstitute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, 420021 Kazan, RussiaInstitute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), 410049 Saratov, Russia<i>Erwinia</i> are widely known as phytopathogenic bacteria, but among them, there are also plant-friendly strains that can promote plant growth (PGPR). The <i>Erwinia</i>-like strain OPT-41 was isolated from <i>Triticum aestivum</i> seedlings as a potential PGPR. The cells (0.9–1.3 × 1.5–3.1 µm) of this microorganism are Gram-negative, rod-shaped, motile (with peritrichous flagella), and non-spore- and non-capsule-forming. The 16S rRNA gene sequence analyses showed it is located in the <i>Erwiniaceae</i> family and has a pairwise similarity above the species delineation threshold of 98.65% with several of its members: <i>Erwinia tasmaniensis</i> (99.21%), <i>Candidatus</i> Pantoea bathycoeliae (98.93%), <i>Pantoea agglomerans</i> (98.87%), <i>Erwinia endophytica</i> (98.83%), <i>Erwinia persicina</i> (98.82%), <i>Erwinia billingiae</i> (98.76%) and <i>Erwinia aphidicola</i> (98.75%). Whole genome-based taxonomy performed on the Type (Strain) Genome Server clarified the status of strain OPT-41, detecting it as a potential new species in the genus <i>Erwinia</i>. The microorganism under study was the most closely related to the type strain of <i>E. phyllosphaerae</i>, demonstrating 27.2% similarity in dDDH, 83.44% similarity in OrthoANIu, and 1.9% difference in G+C content. The major fatty acids of strain OPT-41 were 9 C<sub>16:1</sub>, C<sub>14:0</sub>, and C<sub>16:0</sub>. A combination of genome-based taxonomy and traditional polyphasic taxonomy clearly indicated that strain OPT-41 belongs to a novel <i>Erwinia</i> species, for which the name <i>E. plantamica</i> sp. nov was proposed. OPT-41 (=IBPPM 712=VKM B-3873D=CCTCC AB 2024361) has been designated as the type strain. In addition, OPT-41 was found to have low degradation potential for host plant pectins and proteins and be friendly in <i>Triticum aestivum</i> and <i>Hordeum vulgare</i> crops.https://www.mdpi.com/2076-2607/13/3/474<i>Erwinia plantamica</i><i>Triticum aestivum</i><i>Hordeum vulgare</i>plant-friendly |
| spellingShingle | Anna Egorshina Mikhail Lukyantsev Sergey Golubev Eugenia Boulygina Irina Khilyas Anna Muratova <i>Erwinia plantamica</i> sp. nov., a Non-Phytopathogenic Bacterium Isolated from the Seedlings of Spring Wheat (<i>Triticum aestivum</i> L.) Microorganisms <i>Erwinia plantamica</i> <i>Triticum aestivum</i> <i>Hordeum vulgare</i> plant-friendly |
| title | <i>Erwinia plantamica</i> sp. nov., a Non-Phytopathogenic Bacterium Isolated from the Seedlings of Spring Wheat (<i>Triticum aestivum</i> L.) |
| title_full | <i>Erwinia plantamica</i> sp. nov., a Non-Phytopathogenic Bacterium Isolated from the Seedlings of Spring Wheat (<i>Triticum aestivum</i> L.) |
| title_fullStr | <i>Erwinia plantamica</i> sp. nov., a Non-Phytopathogenic Bacterium Isolated from the Seedlings of Spring Wheat (<i>Triticum aestivum</i> L.) |
| title_full_unstemmed | <i>Erwinia plantamica</i> sp. nov., a Non-Phytopathogenic Bacterium Isolated from the Seedlings of Spring Wheat (<i>Triticum aestivum</i> L.) |
| title_short | <i>Erwinia plantamica</i> sp. nov., a Non-Phytopathogenic Bacterium Isolated from the Seedlings of Spring Wheat (<i>Triticum aestivum</i> L.) |
| title_sort | i erwinia plantamica i sp nov a non phytopathogenic bacterium isolated from the seedlings of spring wheat i triticum aestivum i l |
| topic | <i>Erwinia plantamica</i> <i>Triticum aestivum</i> <i>Hordeum vulgare</i> plant-friendly |
| url | https://www.mdpi.com/2076-2607/13/3/474 |
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