Effect of Berberine Hydrochloride on Disposition Characteristics of Ciprofloxacin Hydrochloride and Its Mechanism in Yellow Catfish (<i>Pelteobagrus fulvidraco</i>) Following Combined Oral Administration

To investigate the effects and underlying mechanisms of the Chinese herbal medicine berberine hydrochloride (BBH) on the pharmacokinetics of the antibiotic ciprofloxacin hydrochloride (CIP) in yellow catfish (<i>Pelteobagrus fulvidraco</i>), this study established two experimental groups...

Full description

Saved in:
Bibliographic Details
Main Authors: Tianfu Zhong, Xiangxuan Du, Yueyan Chen, Yongtao Liu
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Fishes
Subjects:
Online Access:https://www.mdpi.com/2410-3888/10/6/245
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849432232134967296
author Tianfu Zhong
Xiangxuan Du
Yueyan Chen
Yongtao Liu
author_facet Tianfu Zhong
Xiangxuan Du
Yueyan Chen
Yongtao Liu
author_sort Tianfu Zhong
collection DOAJ
description To investigate the effects and underlying mechanisms of the Chinese herbal medicine berberine hydrochloride (BBH) on the pharmacokinetics of the antibiotic ciprofloxacin hydrochloride (CIP) in yellow catfish (<i>Pelteobagrus fulvidraco</i>), this study established two experimental groups: CIP alone and CIP combined with BBH. After administering the two treatment groups, we analyzed the pharmacokinetic characteristics and tissue distribution of CIP in yellow catfish, as well as the differences in the expression levels of two key genes involved in drug disposition—<i>ABCB4</i> (ATP-binding cassette subfamily B member 4, related to drug transport) and <i>CYP3A40</i> (cytochrome P450 3A40, related to drug metabolism)—in the intestinal tract. The results demonstrated that co-administration of CIP and BBH increased the maximum concentration (C<sub>max</sub>) and area under the concentration–time curve (AUC) of CIP while reducing its total body clearance (CL/F). Regarding gene expression, the combined treatment significantly downregulated <i>ABCB4</i> expression in the intestine at certain time points compared to CIP alone, whereas <i>CYP3A40</i> expression showed a non-significant decreasing trend. These findings suggest that BBH may enhance the absorption of CIP in yellow catfish by suppressing <i>ABCB4</i> expression, thereby improving therapeutic efficacy at the same dosage.
format Article
id doaj-art-d4c88ae69e414e349ba01d6be90d5d67
institution Kabale University
issn 2410-3888
language English
publishDate 2025-05-01
publisher MDPI AG
record_format Article
series Fishes
spelling doaj-art-d4c88ae69e414e349ba01d6be90d5d672025-08-20T03:27:25ZengMDPI AGFishes2410-38882025-05-0110624510.3390/fishes10060245Effect of Berberine Hydrochloride on Disposition Characteristics of Ciprofloxacin Hydrochloride and Its Mechanism in Yellow Catfish (<i>Pelteobagrus fulvidraco</i>) Following Combined Oral AdministrationTianfu Zhong0Xiangxuan Du1Yueyan Chen2Yongtao Liu3College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, ChinaTo investigate the effects and underlying mechanisms of the Chinese herbal medicine berberine hydrochloride (BBH) on the pharmacokinetics of the antibiotic ciprofloxacin hydrochloride (CIP) in yellow catfish (<i>Pelteobagrus fulvidraco</i>), this study established two experimental groups: CIP alone and CIP combined with BBH. After administering the two treatment groups, we analyzed the pharmacokinetic characteristics and tissue distribution of CIP in yellow catfish, as well as the differences in the expression levels of two key genes involved in drug disposition—<i>ABCB4</i> (ATP-binding cassette subfamily B member 4, related to drug transport) and <i>CYP3A40</i> (cytochrome P450 3A40, related to drug metabolism)—in the intestinal tract. The results demonstrated that co-administration of CIP and BBH increased the maximum concentration (C<sub>max</sub>) and area under the concentration–time curve (AUC) of CIP while reducing its total body clearance (CL/F). Regarding gene expression, the combined treatment significantly downregulated <i>ABCB4</i> expression in the intestine at certain time points compared to CIP alone, whereas <i>CYP3A40</i> expression showed a non-significant decreasing trend. These findings suggest that BBH may enhance the absorption of CIP in yellow catfish by suppressing <i>ABCB4</i> expression, thereby improving therapeutic efficacy at the same dosage.https://www.mdpi.com/2410-3888/10/6/245berberine hydrochlorideciprofloxacin hydrochloridepharmacokinetics<i>ABCB4</i>yellow catfish
spellingShingle Tianfu Zhong
Xiangxuan Du
Yueyan Chen
Yongtao Liu
Effect of Berberine Hydrochloride on Disposition Characteristics of Ciprofloxacin Hydrochloride and Its Mechanism in Yellow Catfish (<i>Pelteobagrus fulvidraco</i>) Following Combined Oral Administration
Fishes
berberine hydrochloride
ciprofloxacin hydrochloride
pharmacokinetics
<i>ABCB4</i>
yellow catfish
title Effect of Berberine Hydrochloride on Disposition Characteristics of Ciprofloxacin Hydrochloride and Its Mechanism in Yellow Catfish (<i>Pelteobagrus fulvidraco</i>) Following Combined Oral Administration
title_full Effect of Berberine Hydrochloride on Disposition Characteristics of Ciprofloxacin Hydrochloride and Its Mechanism in Yellow Catfish (<i>Pelteobagrus fulvidraco</i>) Following Combined Oral Administration
title_fullStr Effect of Berberine Hydrochloride on Disposition Characteristics of Ciprofloxacin Hydrochloride and Its Mechanism in Yellow Catfish (<i>Pelteobagrus fulvidraco</i>) Following Combined Oral Administration
title_full_unstemmed Effect of Berberine Hydrochloride on Disposition Characteristics of Ciprofloxacin Hydrochloride and Its Mechanism in Yellow Catfish (<i>Pelteobagrus fulvidraco</i>) Following Combined Oral Administration
title_short Effect of Berberine Hydrochloride on Disposition Characteristics of Ciprofloxacin Hydrochloride and Its Mechanism in Yellow Catfish (<i>Pelteobagrus fulvidraco</i>) Following Combined Oral Administration
title_sort effect of berberine hydrochloride on disposition characteristics of ciprofloxacin hydrochloride and its mechanism in yellow catfish i pelteobagrus fulvidraco i following combined oral administration
topic berberine hydrochloride
ciprofloxacin hydrochloride
pharmacokinetics
<i>ABCB4</i>
yellow catfish
url https://www.mdpi.com/2410-3888/10/6/245
work_keys_str_mv AT tianfuzhong effectofberberinehydrochlorideondispositioncharacteristicsofciprofloxacinhydrochlorideanditsmechanisminyellowcatfishipelteobagrusfulvidracoifollowingcombinedoraladministration
AT xiangxuandu effectofberberinehydrochlorideondispositioncharacteristicsofciprofloxacinhydrochlorideanditsmechanisminyellowcatfishipelteobagrusfulvidracoifollowingcombinedoraladministration
AT yueyanchen effectofberberinehydrochlorideondispositioncharacteristicsofciprofloxacinhydrochlorideanditsmechanisminyellowcatfishipelteobagrusfulvidracoifollowingcombinedoraladministration
AT yongtaoliu effectofberberinehydrochlorideondispositioncharacteristicsofciprofloxacinhydrochlorideanditsmechanisminyellowcatfishipelteobagrusfulvidracoifollowingcombinedoraladministration