A cell atlas of the human fallopian tube throughout the menstrual cycle and menopause
Abstract The fallopian tube undergoes extensive molecular changes during the menstrual cycle and menopause. We use single-cell RNA and ATAC sequencing to construct a comprehensive cell atlas of healthy human fallopian tubes during the menstrual cycle and menopause. Our scRNA-seq comparison of 85,107...
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Nature Portfolio
2025-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-55440-2 |
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| author | Melanie Weigert Yan Li Lisha Zhu Heather Eckart Preety Bajwa Rahul Krishnan Sarah Ackroyd Ricardo Lastra Agnes Bilecz Anindita Basu Ernst Lengyel Mengjie Chen |
| author_facet | Melanie Weigert Yan Li Lisha Zhu Heather Eckart Preety Bajwa Rahul Krishnan Sarah Ackroyd Ricardo Lastra Agnes Bilecz Anindita Basu Ernst Lengyel Mengjie Chen |
| author_sort | Melanie Weigert |
| collection | DOAJ |
| description | Abstract The fallopian tube undergoes extensive molecular changes during the menstrual cycle and menopause. We use single-cell RNA and ATAC sequencing to construct a comprehensive cell atlas of healthy human fallopian tubes during the menstrual cycle and menopause. Our scRNA-seq comparison of 85,107 pre- and 46,111 post-menopausal fallopian tube cells reveals substantial shifts in cell type frequencies, gene expression, transcription factor activity, and cell-to-cell communications during menopause and menstrual cycle. Menstrual cycle dependent hormonal changes regulate distinct molecular states in fallopian tube secretory epithelial cells. Postmenopausal fallopian tubes show high chromatin accessibility in transcription factors associated with aging such as Jun, Fos, and BACH1/2, while hormone receptors were generally downregulated, a small proportion of secretory epithelial cells had high expression of ESR2, IGF1R, and LEPR. While a pre-menopausal secretory epithelial gene cluster is enriched in the immunoreactive molecular subtype, a subset of genes expressed in post-menopausal secretory epithelial cells show enrichment in the mesenchymal molecular type of high-grade serous ovarian cancer. |
| format | Article |
| id | doaj-art-d4c4ea64705b48cc94f7c559f55ddadb |
| institution | OA Journals |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-d4c4ea64705b48cc94f7c559f55ddadb2025-08-20T01:48:07ZengNature PortfolioNature Communications2041-17232025-01-0116111510.1038/s41467-024-55440-2A cell atlas of the human fallopian tube throughout the menstrual cycle and menopauseMelanie Weigert0Yan Li1Lisha Zhu2Heather Eckart3Preety Bajwa4Rahul Krishnan5Sarah Ackroyd6Ricardo Lastra7Agnes Bilecz8Anindita Basu9Ernst Lengyel10Mengjie Chen11Department of Obstetrics and Gynecology, Section of Gynecologic Oncology, The University of ChicagoCenter for Research Informatics, The University of ChicagoCenter for Research Informatics, The University of ChicagoDepartment of Medicine, Section of Genetic Medicine, The University of ChicagoDepartment of Obstetrics and Gynecology, Section of Gynecologic Oncology, The University of ChicagoDepartment of Obstetrics and Gynecology, Section of Gynecologic Oncology, The University of ChicagoDepartment of Obstetrics and Gynecology, Section of Gynecologic Oncology, The University of ChicagoDepartment of Pathology, The University of ChicagoDepartment of Obstetrics and Gynecology, Section of Gynecologic Oncology, The University of ChicagoDepartment of Medicine, Section of Genetic Medicine, The University of ChicagoDepartment of Obstetrics and Gynecology, Section of Gynecologic Oncology, The University of ChicagoCenter for Research Informatics, The University of ChicagoAbstract The fallopian tube undergoes extensive molecular changes during the menstrual cycle and menopause. We use single-cell RNA and ATAC sequencing to construct a comprehensive cell atlas of healthy human fallopian tubes during the menstrual cycle and menopause. Our scRNA-seq comparison of 85,107 pre- and 46,111 post-menopausal fallopian tube cells reveals substantial shifts in cell type frequencies, gene expression, transcription factor activity, and cell-to-cell communications during menopause and menstrual cycle. Menstrual cycle dependent hormonal changes regulate distinct molecular states in fallopian tube secretory epithelial cells. Postmenopausal fallopian tubes show high chromatin accessibility in transcription factors associated with aging such as Jun, Fos, and BACH1/2, while hormone receptors were generally downregulated, a small proportion of secretory epithelial cells had high expression of ESR2, IGF1R, and LEPR. While a pre-menopausal secretory epithelial gene cluster is enriched in the immunoreactive molecular subtype, a subset of genes expressed in post-menopausal secretory epithelial cells show enrichment in the mesenchymal molecular type of high-grade serous ovarian cancer.https://doi.org/10.1038/s41467-024-55440-2 |
| spellingShingle | Melanie Weigert Yan Li Lisha Zhu Heather Eckart Preety Bajwa Rahul Krishnan Sarah Ackroyd Ricardo Lastra Agnes Bilecz Anindita Basu Ernst Lengyel Mengjie Chen A cell atlas of the human fallopian tube throughout the menstrual cycle and menopause Nature Communications |
| title | A cell atlas of the human fallopian tube throughout the menstrual cycle and menopause |
| title_full | A cell atlas of the human fallopian tube throughout the menstrual cycle and menopause |
| title_fullStr | A cell atlas of the human fallopian tube throughout the menstrual cycle and menopause |
| title_full_unstemmed | A cell atlas of the human fallopian tube throughout the menstrual cycle and menopause |
| title_short | A cell atlas of the human fallopian tube throughout the menstrual cycle and menopause |
| title_sort | cell atlas of the human fallopian tube throughout the menstrual cycle and menopause |
| url | https://doi.org/10.1038/s41467-024-55440-2 |
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