Green Nanoparticle Synthesis in the Application of Non-Bacterial Mastitis in Cattle
This study explores the potential of silver nanoparticles (AgNPs) synthesized through an eco-friendly method using coffee extract to combat non-bacterial mastitis in dairy cattle. Mastitis, often caused by pathogens such as yeasts and algae like <i>Prototheca</i> spp., poses a challenge...
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MDPI AG
2025-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/30/6/1369 |
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| author | Michał Motrenko Agata Lange Aleksandra Kalińska Marcin Gołębiewski Małgorzata Kunowska-Slósarz Barbara Nasiłowska Joanna Czwartos Wojciech Skrzeczanowski Aleksandra Orzeszko-Rywka Tomasz Jagielski Anna Hotowy Mateusz Wierzbicki Sławomir Jaworski |
| author_facet | Michał Motrenko Agata Lange Aleksandra Kalińska Marcin Gołębiewski Małgorzata Kunowska-Slósarz Barbara Nasiłowska Joanna Czwartos Wojciech Skrzeczanowski Aleksandra Orzeszko-Rywka Tomasz Jagielski Anna Hotowy Mateusz Wierzbicki Sławomir Jaworski |
| author_sort | Michał Motrenko |
| collection | DOAJ |
| description | This study explores the potential of silver nanoparticles (AgNPs) synthesized through an eco-friendly method using coffee extract to combat non-bacterial mastitis in dairy cattle. Mastitis, often caused by pathogens such as yeasts and algae like <i>Prototheca</i> spp., poses a challenge due to the limited efficacy of traditional antibiotics. This research utilized strains isolated from mastitis milk and assessed the nanoparticles’ physicochemical properties, antimicrobial efficacy, and impact on biofilm formation and microorganism invasion. AgNPs demonstrated a spherical shape with a mean hydrodynamic diameter of ~87 nm and moderate colloidal stability. Antimicrobial tests revealed significant growth inhibition of yeast and <i>Prototheca</i> spp., with minimal inhibitory concentrations (MICs) as low as 10 mg/L for certain strains. Biofilm formation was notably disrupted, and microorganism invasion in bioprinted gels was significantly reduced, indicating the broad-spectrum potential of AgNPs. The study highlights the nanoparticles’ ability to damage cell membranes and inhibit metabolic activities, presenting a promising alternative for managing infections resistant to conventional treatments. These findings suggest that green-synthesized AgNPs could play a pivotal role in developing sustainable solutions for mastitis treatment, particularly for pathogens with limited treatment options. |
| format | Article |
| id | doaj-art-d4bec28ae3af41bdb2c76ea3dba37659 |
| institution | Kabale University |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-d4bec28ae3af41bdb2c76ea3dba376592025-08-20T03:43:20ZengMDPI AGMolecules1420-30492025-03-01306136910.3390/molecules30061369Green Nanoparticle Synthesis in the Application of Non-Bacterial Mastitis in CattleMichał Motrenko0Agata Lange1Aleksandra Kalińska2Marcin Gołębiewski3Małgorzata Kunowska-Slósarz4Barbara Nasiłowska5Joanna Czwartos6Wojciech Skrzeczanowski7Aleksandra Orzeszko-Rywka8Tomasz Jagielski9Anna Hotowy10Mateusz Wierzbicki11Sławomir Jaworski12Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-786 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-786 Warsaw, PolandAnimal Breeding Department, Warsaw University of Life Sciences, 02-786 Warsaw, PolandAnimal Breeding Department, Warsaw University of Life Sciences, 02-786 Warsaw, PolandAnimal Breeding Department, Warsaw University of Life Sciences, 02-786 Warsaw, PolandInstitute of Optoelectronics, Military University of Technology, 00-908 Warsaw, PolandInstitute of Optoelectronics, Military University of Technology, 00-908 Warsaw, PolandInstitute of Optoelectronics, Military University of Technology, 00-908 Warsaw, PolandDepartment of Plant Physiology, Institute of Biology, 02-776 Warsaw, PolandDepartment of Applied Microbiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, 00-927 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-786 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-786 Warsaw, PolandDepartment of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-786 Warsaw, PolandThis study explores the potential of silver nanoparticles (AgNPs) synthesized through an eco-friendly method using coffee extract to combat non-bacterial mastitis in dairy cattle. Mastitis, often caused by pathogens such as yeasts and algae like <i>Prototheca</i> spp., poses a challenge due to the limited efficacy of traditional antibiotics. This research utilized strains isolated from mastitis milk and assessed the nanoparticles’ physicochemical properties, antimicrobial efficacy, and impact on biofilm formation and microorganism invasion. AgNPs demonstrated a spherical shape with a mean hydrodynamic diameter of ~87 nm and moderate colloidal stability. Antimicrobial tests revealed significant growth inhibition of yeast and <i>Prototheca</i> spp., with minimal inhibitory concentrations (MICs) as low as 10 mg/L for certain strains. Biofilm formation was notably disrupted, and microorganism invasion in bioprinted gels was significantly reduced, indicating the broad-spectrum potential of AgNPs. The study highlights the nanoparticles’ ability to damage cell membranes and inhibit metabolic activities, presenting a promising alternative for managing infections resistant to conventional treatments. These findings suggest that green-synthesized AgNPs could play a pivotal role in developing sustainable solutions for mastitis treatment, particularly for pathogens with limited treatment options.https://www.mdpi.com/1420-3049/30/6/1369yeasts<i>Prototheca</i> spp.mastitisgreen synthesissilver nanoparticles |
| spellingShingle | Michał Motrenko Agata Lange Aleksandra Kalińska Marcin Gołębiewski Małgorzata Kunowska-Slósarz Barbara Nasiłowska Joanna Czwartos Wojciech Skrzeczanowski Aleksandra Orzeszko-Rywka Tomasz Jagielski Anna Hotowy Mateusz Wierzbicki Sławomir Jaworski Green Nanoparticle Synthesis in the Application of Non-Bacterial Mastitis in Cattle Molecules yeasts <i>Prototheca</i> spp. mastitis green synthesis silver nanoparticles |
| title | Green Nanoparticle Synthesis in the Application of Non-Bacterial Mastitis in Cattle |
| title_full | Green Nanoparticle Synthesis in the Application of Non-Bacterial Mastitis in Cattle |
| title_fullStr | Green Nanoparticle Synthesis in the Application of Non-Bacterial Mastitis in Cattle |
| title_full_unstemmed | Green Nanoparticle Synthesis in the Application of Non-Bacterial Mastitis in Cattle |
| title_short | Green Nanoparticle Synthesis in the Application of Non-Bacterial Mastitis in Cattle |
| title_sort | green nanoparticle synthesis in the application of non bacterial mastitis in cattle |
| topic | yeasts <i>Prototheca</i> spp. mastitis green synthesis silver nanoparticles |
| url | https://www.mdpi.com/1420-3049/30/6/1369 |
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