A global S. cerevisiae small ubiquitin‐related modifier (SUMO) system interactome
Abstract The small ubiquitin‐related modifier (SUMO) system has been implicated in a number of biological functions, yet the individual components of the SUMO machinery involved in each of these activities were largely unknown. Here we report the first global SUMO system interactome. Using affinity...
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| Format: | Article |
| Language: | English |
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Springer Nature
2013-05-01
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| Series: | Molecular Systems Biology |
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| Online Access: | https://doi.org/10.1038/msb.2013.23 |
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| _version_ | 1849389459613679616 |
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| author | Tharan Srikumar Megan C Lewicki Brian Raught |
| author_facet | Tharan Srikumar Megan C Lewicki Brian Raught |
| author_sort | Tharan Srikumar |
| collection | DOAJ |
| description | Abstract The small ubiquitin‐related modifier (SUMO) system has been implicated in a number of biological functions, yet the individual components of the SUMO machinery involved in each of these activities were largely unknown. Here we report the first global SUMO system interactome. Using affinity purification coupled with mass spectrometry, we identify >450 protein–protein interactions surrounding the SUMO E2, Siz type E3s and SUMO‐specific proteases in budding yeast. Exploiting this information‐rich resource, we validate several Siz1‐ and Siz2‐specific substrates, identify a nucleoporin required for proper Ulp1 localization, and uncover important new roles for Ubc9 and Ulp2 in the maintenance of ribosomal DNA. |
| format | Article |
| id | doaj-art-d4b245fec4884509aa379b8513d34963 |
| institution | Kabale University |
| issn | 1744-4292 |
| language | English |
| publishDate | 2013-05-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj-art-d4b245fec4884509aa379b8513d349632025-08-20T03:41:57ZengSpringer NatureMolecular Systems Biology1744-42922013-05-01911910.1038/msb.2013.23A global S. cerevisiae small ubiquitin‐related modifier (SUMO) system interactomeTharan Srikumar0Megan C Lewicki1Brian Raught2Ontario Cancer Institute, University Health Network and Department of Medical Biophysics, University of TorontoOntario Cancer Institute, University Health Network and Department of Medical Biophysics, University of TorontoOntario Cancer Institute, University Health Network and Department of Medical Biophysics, University of TorontoAbstract The small ubiquitin‐related modifier (SUMO) system has been implicated in a number of biological functions, yet the individual components of the SUMO machinery involved in each of these activities were largely unknown. Here we report the first global SUMO system interactome. Using affinity purification coupled with mass spectrometry, we identify >450 protein–protein interactions surrounding the SUMO E2, Siz type E3s and SUMO‐specific proteases in budding yeast. Exploiting this information‐rich resource, we validate several Siz1‐ and Siz2‐specific substrates, identify a nucleoporin required for proper Ulp1 localization, and uncover important new roles for Ubc9 and Ulp2 in the maintenance of ribosomal DNA.https://doi.org/10.1038/msb.2013.23affinity purification‐mass spectrometrymass spectrometryribosomal DNAS. cerevisiaeSUMO |
| spellingShingle | Tharan Srikumar Megan C Lewicki Brian Raught A global S. cerevisiae small ubiquitin‐related modifier (SUMO) system interactome Molecular Systems Biology affinity purification‐mass spectrometry mass spectrometry ribosomal DNA S. cerevisiae SUMO |
| title | A global S. cerevisiae small ubiquitin‐related modifier (SUMO) system interactome |
| title_full | A global S. cerevisiae small ubiquitin‐related modifier (SUMO) system interactome |
| title_fullStr | A global S. cerevisiae small ubiquitin‐related modifier (SUMO) system interactome |
| title_full_unstemmed | A global S. cerevisiae small ubiquitin‐related modifier (SUMO) system interactome |
| title_short | A global S. cerevisiae small ubiquitin‐related modifier (SUMO) system interactome |
| title_sort | global s cerevisiae small ubiquitin related modifier sumo system interactome |
| topic | affinity purification‐mass spectrometry mass spectrometry ribosomal DNA S. cerevisiae SUMO |
| url | https://doi.org/10.1038/msb.2013.23 |
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