Composition and toxicity of total water-soluble flavonoids from Lithocarpus litseifolius (Hance) Chun (sweet tea)
The material basis and toxicity of Lithocarpus litseifolius (Hance) Chun (sweet tea), which has many health benefits, are not fully understood. In this study, total water-soluble flavonoids were extracted from sweet tea using optimised methods based on RSM. Its composition and toxicity were investig...
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Taylor & Francis Group
2024-12-01
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| Series: | Food and Agricultural Immunology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/09540105.2024.2358801 |
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| author | Junfei Xu Yingqi Zhao Jing Wen Ye Lin Yangtao Li Yijun He Xianjin Wu Juzuo Zhang |
| author_facet | Junfei Xu Yingqi Zhao Jing Wen Ye Lin Yangtao Li Yijun He Xianjin Wu Juzuo Zhang |
| author_sort | Junfei Xu |
| collection | DOAJ |
| description | The material basis and toxicity of Lithocarpus litseifolius (Hance) Chun (sweet tea), which has many health benefits, are not fully understood. In this study, total water-soluble flavonoids were extracted from sweet tea using optimised methods based on RSM. Its composition and toxicity were investigated by LC–MS and mouse models in vivo. The results showed that the optimum extraction conditions were a liquid/solid ratio of 32.98:1 ml/g, a temperature of 60.69°C, a microwave power of 633.70 W, and a microwave time of 164.08 secs. Among the 33 main components in the extract, five flavonoids constituted more than 73.80% of the total compound content. Total flavonoids were safe after oral administration and alleviated reproductive disorders but were highly toxic after intraperitoneal injection in mice. In summary, under the optimum extraction conditions, the total water-soluble flavonoids were safe after oral administration, but the complex compounds hindered their direct injection.Abbreviations: LLHC: Lithocarpus litseifolius (Hance) Chun; SFD: single-factor design; RSM: response surface methodology; CCD: central composite design; ES: extracted solution; CS concentrated solution; LP: lyophilised powder; LC–MS: liquid chromatography–mass spectrometry; NC: negative control; PH: procaine hydrochloride; CP: cyclophosphamide group. |
| format | Article |
| id | doaj-art-d4ae18fdaecd4edbb4d236e4c832a887 |
| institution | OA Journals |
| issn | 0954-0105 1465-3443 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj-art-d4ae18fdaecd4edbb4d236e4c832a8872025-08-20T01:58:15ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432024-12-0135110.1080/09540105.2024.2358801Composition and toxicity of total water-soluble flavonoids from Lithocarpus litseifolius (Hance) Chun (sweet tea)Junfei Xu0Yingqi Zhao1Jing Wen2Ye Lin3Yangtao Li4Yijun He5Xianjin Wu6Juzuo Zhang7College of Biological and Food Engineering/Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province/Hunan Provincial Higher Education Key Laboratory of Intensive Processing Research on Mountain Ecological Food, Huaihua University, Huaihua, People’s Republic of ChinaHunan Jinzhou Pharmaceutical Co., Ltd., Huaihua, People’s Republic of ChinaCollege of Biological and Food Engineering/Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province/Hunan Provincial Higher Education Key Laboratory of Intensive Processing Research on Mountain Ecological Food, Huaihua University, Huaihua, People’s Republic of ChinaCollege of Biological and Food Engineering/Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province/Hunan Provincial Higher Education Key Laboratory of Intensive Processing Research on Mountain Ecological Food, Huaihua University, Huaihua, People’s Republic of ChinaCollege of Biological and Food Engineering/Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province/Hunan Provincial Higher Education Key Laboratory of Intensive Processing Research on Mountain Ecological Food, Huaihua University, Huaihua, People’s Republic of ChinaCollege of Biological and Food Engineering/Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province/Hunan Provincial Higher Education Key Laboratory of Intensive Processing Research on Mountain Ecological Food, Huaihua University, Huaihua, People’s Republic of ChinaCollege of Biological and Food Engineering/Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province/Hunan Provincial Higher Education Key Laboratory of Intensive Processing Research on Mountain Ecological Food, Huaihua University, Huaihua, People’s Republic of ChinaCollege of Biological and Food Engineering/Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province/Hunan Provincial Higher Education Key Laboratory of Intensive Processing Research on Mountain Ecological Food, Huaihua University, Huaihua, People’s Republic of ChinaThe material basis and toxicity of Lithocarpus litseifolius (Hance) Chun (sweet tea), which has many health benefits, are not fully understood. In this study, total water-soluble flavonoids were extracted from sweet tea using optimised methods based on RSM. Its composition and toxicity were investigated by LC–MS and mouse models in vivo. The results showed that the optimum extraction conditions were a liquid/solid ratio of 32.98:1 ml/g, a temperature of 60.69°C, a microwave power of 633.70 W, and a microwave time of 164.08 secs. Among the 33 main components in the extract, five flavonoids constituted more than 73.80% of the total compound content. Total flavonoids were safe after oral administration and alleviated reproductive disorders but were highly toxic after intraperitoneal injection in mice. In summary, under the optimum extraction conditions, the total water-soluble flavonoids were safe after oral administration, but the complex compounds hindered their direct injection.Abbreviations: LLHC: Lithocarpus litseifolius (Hance) Chun; SFD: single-factor design; RSM: response surface methodology; CCD: central composite design; ES: extracted solution; CS concentrated solution; LP: lyophilised powder; LC–MS: liquid chromatography–mass spectrometry; NC: negative control; PH: procaine hydrochloride; CP: cyclophosphamide group.https://www.tandfonline.com/doi/10.1080/09540105.2024.2358801Lithocarpus litseifolius (Hance) Chuncompoundswater-soluble flavonoidtoxicity |
| spellingShingle | Junfei Xu Yingqi Zhao Jing Wen Ye Lin Yangtao Li Yijun He Xianjin Wu Juzuo Zhang Composition and toxicity of total water-soluble flavonoids from Lithocarpus litseifolius (Hance) Chun (sweet tea) Food and Agricultural Immunology Lithocarpus litseifolius (Hance) Chun compounds water-soluble flavonoid toxicity |
| title | Composition and toxicity of total water-soluble flavonoids from Lithocarpus litseifolius (Hance) Chun (sweet tea) |
| title_full | Composition and toxicity of total water-soluble flavonoids from Lithocarpus litseifolius (Hance) Chun (sweet tea) |
| title_fullStr | Composition and toxicity of total water-soluble flavonoids from Lithocarpus litseifolius (Hance) Chun (sweet tea) |
| title_full_unstemmed | Composition and toxicity of total water-soluble flavonoids from Lithocarpus litseifolius (Hance) Chun (sweet tea) |
| title_short | Composition and toxicity of total water-soluble flavonoids from Lithocarpus litseifolius (Hance) Chun (sweet tea) |
| title_sort | composition and toxicity of total water soluble flavonoids from lithocarpus litseifolius hance chun sweet tea |
| topic | Lithocarpus litseifolius (Hance) Chun compounds water-soluble flavonoid toxicity |
| url | https://www.tandfonline.com/doi/10.1080/09540105.2024.2358801 |
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