Association of interferon regulatory factor 8 dysregulation with dry eye in Sjögren’s syndrome
AIM: To investigate the expression of interferon regulatory factors (IRFs) in peripheral blood mononuclear cells (PBMCs) of patients with Sjögren's syndrome-related dry eye (SSDE) and to explore their correlation with clinical features, dendritic cell activation, and serological indicators. MET...
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Press of International Journal of Ophthalmology (IJO PRESS)
2025-08-01
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| author | Jiao Wang Guang-Hong Chu Zi-Huan Wang Xiao-Yu Cai Si-Yuan Shi Qi-Ping Qing Qi Zhang |
| author_facet | Jiao Wang Guang-Hong Chu Zi-Huan Wang Xiao-Yu Cai Si-Yuan Shi Qi-Ping Qing Qi Zhang |
| author_sort | Jiao Wang |
| collection | DOAJ |
| description | AIM: To investigate the expression of interferon regulatory factors (IRFs) in peripheral blood mononuclear cells (PBMCs) of patients with Sjögren's syndrome-related dry eye (SSDE) and to explore their correlation with clinical features, dendritic cell activation, and serological indicators. METHODS: A total of 53 SSDE patients and 62 non-Sjögren's syndrome dry eye (NSSDE) patients were enrolled. Demographic and clinical data were collected, and comprehensive ophthalmic examinations were performed, including the ocular surface disease index (OSDI) questionnaires, Schirmer I test (SIT), tear break-up time (TBUT), corneal fluorescein staining score (CFS), and in vivo confocal microscopy (IVCM). PBMCs were isolated, and IRFs expression levels were analyzed using Western blotting (WB) and quantitative real-time polymerase chain reaction (qRT-PCR). Serological indicators, including antinuclear antibodies (ANA) and anti-Ro60, anti-Ro52, and anti-La autoantibodies, were detected. Statistical analyses evaluated correlations between IRFs expression and clinical parameters. RESULTS: Compared to NSSDE, the relative mRNA and protein expression of the IRF-8 was significantly upregulated in patients with SSDE (P<0.001), whereas no significant differences were observed in IRF-1, IRF-3, IRF-5, and IRF-7 (P=0.12, P=0.10, P=0.66, P=0.96). Correlation analysis revealed that IRF-8 expression was positively associated with CFS and OSDI scores (r=0.57, r=0.38, both P<0.05). Moreover, IRF-8 expression correlated with corneal dendritic cell (DC) density and size, and the number of dendrites (r=0.43, r=0.40, r=0.65, all P<0.05). IRF-8 expression was significantly elevated in patients positive for anti-Ro60, anti-Ro52 and anti-La autoantibodies (P<0.05). CONCLUSION: In SSDE, IRF-8 is upregulated and associated with clinical features, DC activation, and serological indicators. These findings suggest that IRF-8 plays a critical role in SSDE pathogenesis and may serve as a potential therapeutic target for diagnosis and treatment. |
| format | Article |
| id | doaj-art-d49e1f09439b4d28b6b3344d1e35b511 |
| institution | Kabale University |
| issn | 2222-3959 2227-4898 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj-art-d49e1f09439b4d28b6b3344d1e35b5112025-08-20T03:51:09ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982025-08-011881456146310.18240/ijo.2025.08.0520250805Association of interferon regulatory factor 8 dysregulation with dry eye in Sjögren’s syndromeJiao Wang0Guang-Hong Chu1Zi-Huan Wang2Xiao-Yu Cai3Si-Yuan Shi4Qi-Ping Qing5Qi Zhang6Qi Zhang. The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing Branch (Municipality Division) of National Clinical Research Center for Ocular Diseases, Chongqing 400016, China. cqzqwxm@163.comThe First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing Branch (Municipality Division) of National Clinical Research Center for Ocular Diseases, Chongqing 400016, China;Co-first Authors: Jiao Wang and Guang-Hong ChuThe First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing Branch (Municipality Division) of National Clinical Research Center for Ocular Diseases, Chongqing 400016, China;Co-first Authors: Jiao Wang and Guang-Hong ChuThe First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing Branch (Municipality Division) of National Clinical Research Center for Ocular Diseases, Chongqing 400016, China;Co-first Authors: Jiao Wang and Guang-Hong ChuThe First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing Branch (Municipality Division) of National Clinical Research Center for Ocular Diseases, Chongqing 400016, China;Co-first Authors: Jiao Wang and Guang-Hong ChuThe First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing Branch (Municipality Division) of National Clinical Research Center for Ocular Diseases, Chongqing 400016, China;Co-first Authors: Jiao Wang and Guang-Hong ChuThe First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing Branch (Municipality Division) of National Clinical Research Center for Ocular Diseases, Chongqing 400016, China;Co-first Authors: Jiao Wang and Guang-Hong ChuAIM: To investigate the expression of interferon regulatory factors (IRFs) in peripheral blood mononuclear cells (PBMCs) of patients with Sjögren's syndrome-related dry eye (SSDE) and to explore their correlation with clinical features, dendritic cell activation, and serological indicators. METHODS: A total of 53 SSDE patients and 62 non-Sjögren's syndrome dry eye (NSSDE) patients were enrolled. Demographic and clinical data were collected, and comprehensive ophthalmic examinations were performed, including the ocular surface disease index (OSDI) questionnaires, Schirmer I test (SIT), tear break-up time (TBUT), corneal fluorescein staining score (CFS), and in vivo confocal microscopy (IVCM). PBMCs were isolated, and IRFs expression levels were analyzed using Western blotting (WB) and quantitative real-time polymerase chain reaction (qRT-PCR). Serological indicators, including antinuclear antibodies (ANA) and anti-Ro60, anti-Ro52, and anti-La autoantibodies, were detected. Statistical analyses evaluated correlations between IRFs expression and clinical parameters. RESULTS: Compared to NSSDE, the relative mRNA and protein expression of the IRF-8 was significantly upregulated in patients with SSDE (P<0.001), whereas no significant differences were observed in IRF-1, IRF-3, IRF-5, and IRF-7 (P=0.12, P=0.10, P=0.66, P=0.96). Correlation analysis revealed that IRF-8 expression was positively associated with CFS and OSDI scores (r=0.57, r=0.38, both P<0.05). Moreover, IRF-8 expression correlated with corneal dendritic cell (DC) density and size, and the number of dendrites (r=0.43, r=0.40, r=0.65, all P<0.05). IRF-8 expression was significantly elevated in patients positive for anti-Ro60, anti-Ro52 and anti-La autoantibodies (P<0.05). CONCLUSION: In SSDE, IRF-8 is upregulated and associated with clinical features, DC activation, and serological indicators. These findings suggest that IRF-8 plays a critical role in SSDE pathogenesis and may serve as a potential therapeutic target for diagnosis and treatment.http://ies.ijo.cn/en_publish/2025/8/20250805.pdfinterferon regulatory factorssjögren's syndromedry eye |
| spellingShingle | Jiao Wang Guang-Hong Chu Zi-Huan Wang Xiao-Yu Cai Si-Yuan Shi Qi-Ping Qing Qi Zhang Association of interferon regulatory factor 8 dysregulation with dry eye in Sjögren’s syndrome International Journal of Ophthalmology interferon regulatory factors sjögren's syndrome dry eye |
| title | Association of interferon regulatory factor 8 dysregulation with dry eye in Sjögren’s syndrome |
| title_full | Association of interferon regulatory factor 8 dysregulation with dry eye in Sjögren’s syndrome |
| title_fullStr | Association of interferon regulatory factor 8 dysregulation with dry eye in Sjögren’s syndrome |
| title_full_unstemmed | Association of interferon regulatory factor 8 dysregulation with dry eye in Sjögren’s syndrome |
| title_short | Association of interferon regulatory factor 8 dysregulation with dry eye in Sjögren’s syndrome |
| title_sort | association of interferon regulatory factor 8 dysregulation with dry eye in sjogren s syndrome |
| topic | interferon regulatory factors sjögren's syndrome dry eye |
| url | http://ies.ijo.cn/en_publish/2025/8/20250805.pdf |
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