Biocontrol Potential of <i>Bacillus stercoris</i> Strain DXQ-1 Against Rice Blast Fungus Guy11
Fungal diseases severely threaten global agriculture, while conventional chemical fungicides face increasing restrictions due to environmental and safety concerns. In this study, we isolated a soil-derived <i>Bacillus stercoris</i> strain, DXQ-1, exhibiting strong antagonistic activity a...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
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| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/7/1538 |
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| Summary: | Fungal diseases severely threaten global agriculture, while conventional chemical fungicides face increasing restrictions due to environmental and safety concerns. In this study, we isolated a soil-derived <i>Bacillus stercoris</i> strain, DXQ-1, exhibiting strong antagonistic activity against plant pathogenic fungi, notably <i>Magnaporthe oryzae</i>, the causal agent of rice blast. Scanning electron microscopy revealed that DXQ-1 disrupts fungal hyphae and inhibits conidial germination, with a 24 h crude broth treatment reducing germination to 83.33% and completely blocking appressoria formation. LC-MS-based metabolomic analysis identified key antifungal components, including lipids (35.83%), organic acid derivatives (22.15%), and small bioactive molecules (e.g., Leu-Pro, LPE 15:0). After optimizing fermentation conditions (LB medium, pH 7.0, 28 °C, 48 h), the broth showed >90% inhibition against <i>M. oryzae</i> and <i>Nigrospora oryzae</i> and retained high thermal (68 °C, 1 h) and UV (4 h) stability. Field trials demonstrated effective disease control and significant promotion of rice growth, increasing plant height (17.7%), fresh weight (53.3%), and dry weight (33.3%). These findings highlight DXQ-1 as a promising biocontrol agent, offering a sustainable and effective alternative for managing fungal diseases in crops. |
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| ISSN: | 2076-2607 |