Exploring the mechanism of Bu Zhong Yi Qi Tang in the treatment of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) based on network pharmacology and molecular docking
Background: Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is a common and persistent condition affecting the male urinary system, with a global prevalence ranging from 2% to 10%. This study aims to investigate the therapeutic mechanisms of Bu Zhong Yi Qi Tang (BZYQT) in treating CP/CPPS...
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| Language: | English |
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MRE Press
2025-03-01
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| Series: | Journal of Men's Health |
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| Online Access: | https://oss.jomh.org/files/article/20250328-512/pdf/JOMH2024090402.pdf |
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| author | Pengfei Zhou Yang Xuan Zaisheng Zhu Han Wu Yang Hu Liangyou Chen Qianya Zhou |
| author_facet | Pengfei Zhou Yang Xuan Zaisheng Zhu Han Wu Yang Hu Liangyou Chen Qianya Zhou |
| author_sort | Pengfei Zhou |
| collection | DOAJ |
| description | Background: Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is a common and persistent condition affecting the male urinary system, with a global prevalence ranging from 2% to 10%. This study aims to investigate the therapeutic mechanisms of Bu Zhong Yi Qi Tang (BZYQT) in treating CP/CPPS using network pharmacology and molecular docking techniques. Methods: The active compounds and their corresponding target proteins of BZYQT were identified and screened using the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). Targets associated with CP/CPPS were determined through GeneCards, the Therapeutic Target Database (TTD), Online Mendelian Inheritance in Man (OMIM), and the Pharmacogenomics Knowledgebase (PharmGKB). Overlapping targets between BZYQT and CP/CPPS were analyzed using the STRING database to construct a protein-protein interaction (PPI) network. Key targets were further subjected to Gene Ontology (GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Molecular docking studies were conducted to validate the interactions between core active compounds and key targets. In vitro experiments were performed to confirm the therapeutic efficacy of BZYQT in treating CP/CPPS. Results: The study identified 103 active compounds in BZYQT and 2064 potential target proteins. A total of 1020 CP/CPPS-related targets were retrieved from GeneCards, OMIM, TTD, and PharmGKB, leading to the identification of 73 overlapping targets and 5 core targets. GO enrichment analysis revealed that these targets are involved in inflammatory responses, apoptosis, oxidative stress, and cell proliferation. KEGG pathway analysis highlighted associations with pathways such as the ErbB signaling pathway. Molecular docking results suggested that kaempferol, an active compound in BZYQT, exhibited the highest binding affinity with the target proteins. Experimental validation demonstrated that kaempferol effectively inhibited the expression of EGFR, MMP9, TNF-α and IL-6. Conclusions: BZYQT exhibits significant therapeutic potential in the treatment of CP/CPPS through its multi-target and multi-pathway mechanisms. The active compound kaempferol plays a crucial role in alleviating pathological changes in CP/CPPS by downregulating the expression of MMP9, EGFR, IL-6, and TNF-α. These findings provide a molecular basis for the application of traditional Chinese medicine in the management of CP/CPPS. |
| format | Article |
| id | doaj-art-d49b8552cbe04777a6774f8876cdcfa3 |
| institution | Kabale University |
| issn | 1875-6867 1875-6859 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MRE Press |
| record_format | Article |
| series | Journal of Men's Health |
| spelling | doaj-art-d49b8552cbe04777a6774f8876cdcfa32025-08-20T03:52:47ZengMRE PressJournal of Men's Health1875-68671875-68592025-03-01213778510.22514/jomh.2025.039S1875-6867(25)00356-2Exploring the mechanism of Bu Zhong Yi Qi Tang in the treatment of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) based on network pharmacology and molecular dockingPengfei Zhou0Yang Xuan1Zaisheng Zhu2Han Wu3Yang Hu4Liangyou Chen5Qianya Zhou6Department of Urology, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, 321000 Jinhua, Zhejiang, ChinaZhejiang Chinese Medical University, 310053 Hangzhou, Zhejiang, ChinaDepartment of Urology, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, 321000 Jinhua, Zhejiang, ChinaDepartment of Urology, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, 321000 Jinhua, Zhejiang, ChinaDepartment of Urology, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, 321000 Jinhua, Zhejiang, ChinaDepartment of Urology, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, 321000 Jinhua, Zhejiang, ChinaZhejiang Chinese Medical University, 310053 Hangzhou, Zhejiang, ChinaBackground: Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is a common and persistent condition affecting the male urinary system, with a global prevalence ranging from 2% to 10%. This study aims to investigate the therapeutic mechanisms of Bu Zhong Yi Qi Tang (BZYQT) in treating CP/CPPS using network pharmacology and molecular docking techniques. Methods: The active compounds and their corresponding target proteins of BZYQT were identified and screened using the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). Targets associated with CP/CPPS were determined through GeneCards, the Therapeutic Target Database (TTD), Online Mendelian Inheritance in Man (OMIM), and the Pharmacogenomics Knowledgebase (PharmGKB). Overlapping targets between BZYQT and CP/CPPS were analyzed using the STRING database to construct a protein-protein interaction (PPI) network. Key targets were further subjected to Gene Ontology (GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Molecular docking studies were conducted to validate the interactions between core active compounds and key targets. In vitro experiments were performed to confirm the therapeutic efficacy of BZYQT in treating CP/CPPS. Results: The study identified 103 active compounds in BZYQT and 2064 potential target proteins. A total of 1020 CP/CPPS-related targets were retrieved from GeneCards, OMIM, TTD, and PharmGKB, leading to the identification of 73 overlapping targets and 5 core targets. GO enrichment analysis revealed that these targets are involved in inflammatory responses, apoptosis, oxidative stress, and cell proliferation. KEGG pathway analysis highlighted associations with pathways such as the ErbB signaling pathway. Molecular docking results suggested that kaempferol, an active compound in BZYQT, exhibited the highest binding affinity with the target proteins. Experimental validation demonstrated that kaempferol effectively inhibited the expression of EGFR, MMP9, TNF-α and IL-6. Conclusions: BZYQT exhibits significant therapeutic potential in the treatment of CP/CPPS through its multi-target and multi-pathway mechanisms. The active compound kaempferol plays a crucial role in alleviating pathological changes in CP/CPPS by downregulating the expression of MMP9, EGFR, IL-6, and TNF-α. These findings provide a molecular basis for the application of traditional Chinese medicine in the management of CP/CPPS.https://oss.jomh.org/files/article/20250328-512/pdf/JOMH2024090402.pdfbzyqtchronic prostatitischronic pelvic pain syndromenetwork pharmacology |
| spellingShingle | Pengfei Zhou Yang Xuan Zaisheng Zhu Han Wu Yang Hu Liangyou Chen Qianya Zhou Exploring the mechanism of Bu Zhong Yi Qi Tang in the treatment of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) based on network pharmacology and molecular docking Journal of Men's Health bzyqt chronic prostatitis chronic pelvic pain syndrome network pharmacology |
| title | Exploring the mechanism of Bu Zhong Yi Qi Tang in the treatment of
chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) based on network
pharmacology and molecular docking |
| title_full | Exploring the mechanism of Bu Zhong Yi Qi Tang in the treatment of
chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) based on network
pharmacology and molecular docking |
| title_fullStr | Exploring the mechanism of Bu Zhong Yi Qi Tang in the treatment of
chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) based on network
pharmacology and molecular docking |
| title_full_unstemmed | Exploring the mechanism of Bu Zhong Yi Qi Tang in the treatment of
chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) based on network
pharmacology and molecular docking |
| title_short | Exploring the mechanism of Bu Zhong Yi Qi Tang in the treatment of
chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) based on network
pharmacology and molecular docking |
| title_sort | exploring the mechanism of bu zhong yi qi tang in the treatment of chronic prostatitis chronic pelvic pain syndrome cp cpps based on network pharmacology and molecular docking |
| topic | bzyqt chronic prostatitis chronic pelvic pain syndrome network pharmacology |
| url | https://oss.jomh.org/files/article/20250328-512/pdf/JOMH2024090402.pdf |
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