Race-related host and microbe transcriptomic signatures in triple-negative breast cancer
Abstract Triple-negative breast cancer (TNBC) shows racial disparities, with higher incidence in women of African ancestry (AA) compared to European ancestry (EA). Meta-transcriptomic analysis of TNBC tumor tissues from AA (n = 17) and EA (n = 19) subjects revealed distinct microbial landscapes. Hie...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-08-01
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| Series: | npj Breast Cancer |
| Online Access: | https://doi.org/10.1038/s41523-025-00806-y |
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| author | Roshan Kumar Susan Duyar-Ayerdi Aishwarya Sundaresan Vinodh Srinivasasainagendra Chandra Sekhar Pedamallu Michael Behring Darshan Shimoga Chandrashekar Isam-Eldin Eltoum Sooryanarayana Varambally Hemant K. Tiwari Sadeep Shrestha Paul L. Auer Lubna N. Chaudhary John R. Kirby Clayton Yates Upender Manne Akinyemi I. Ojesina |
| author_facet | Roshan Kumar Susan Duyar-Ayerdi Aishwarya Sundaresan Vinodh Srinivasasainagendra Chandra Sekhar Pedamallu Michael Behring Darshan Shimoga Chandrashekar Isam-Eldin Eltoum Sooryanarayana Varambally Hemant K. Tiwari Sadeep Shrestha Paul L. Auer Lubna N. Chaudhary John R. Kirby Clayton Yates Upender Manne Akinyemi I. Ojesina |
| author_sort | Roshan Kumar |
| collection | DOAJ |
| description | Abstract Triple-negative breast cancer (TNBC) shows racial disparities, with higher incidence in women of African ancestry (AA) compared to European ancestry (EA). Meta-transcriptomic analysis of TNBC tumor tissues from AA (n = 17) and EA (n = 19) subjects revealed distinct microbial landscapes. Hierarchical clustering based on microbial transcripts separated samples into two groups predominantly defined by racial ancestry. Bacterial genera including Hafnia and Cedecea were more abundant in AA tumors, while Erwinia was higher in EA tumors. Cellular composition analysis by xCell revealed differences in immune cell populations, with AA tumors having higher Th1 cell abundance and EA tumors containing higher macrophage M2 cell abundance. Nonetheless, AA women with high M2 abundance experienced poorer disease-free survival (DFS) than EA women. Integrative analyses revealed that high expression of human SPDYE2B gene was associated with Hafnia abundance and decreased DFS, highlighting complex host-microbe interactions in TNBC patients. |
| format | Article |
| id | doaj-art-d48efed9e5cc4532ab975a3fbe0f8839 |
| institution | Kabale University |
| issn | 2374-4677 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Breast Cancer |
| spelling | doaj-art-d48efed9e5cc4532ab975a3fbe0f88392025-08-20T03:46:25ZengNature Portfolionpj Breast Cancer2374-46772025-08-0111111010.1038/s41523-025-00806-yRace-related host and microbe transcriptomic signatures in triple-negative breast cancerRoshan Kumar0Susan Duyar-Ayerdi1Aishwarya Sundaresan2Vinodh Srinivasasainagendra3Chandra Sekhar Pedamallu4Michael Behring5Darshan Shimoga Chandrashekar6Isam-Eldin Eltoum7Sooryanarayana Varambally8Hemant K. Tiwari9Sadeep Shrestha10Paul L. Auer11Lubna N. Chaudhary12John R. Kirby13Clayton Yates14Upender Manne15Akinyemi I. Ojesina16Department of Obstetrics and Gynecology, Medical College of WisconsinDepartment of Obstetrics and Gynecology, Medical College of WisconsinDepartment of Biostatistics, University of Alabama at BirminghamDepartment of Biostatistics, University of Alabama at BirminghamExcelraDepartment of Pathology, University of Alabama at BirminghamDepartment of Pathology, University of Alabama at BirminghamDepartment of Pathology, University of Alabama at BirminghamDepartment of Pathology, University of Alabama at BirminghamDepartment of Biostatistics, University of Alabama at BirminghamDepartment of Epidemiology, University of Alabama at BirminghamMedical College of Wisconsin Cancer CenterDepartment of Medicine, Division of Hematology and Oncology, Medical College of WisconsinDepartment of Microbiology and Immunology, Medical College of WisconsinDepartment of Pathology, Johns Hopkins School of MedicineDepartment of Pathology, University of Alabama at BirminghamDepartment of Obstetrics and Gynecology, Medical College of WisconsinAbstract Triple-negative breast cancer (TNBC) shows racial disparities, with higher incidence in women of African ancestry (AA) compared to European ancestry (EA). Meta-transcriptomic analysis of TNBC tumor tissues from AA (n = 17) and EA (n = 19) subjects revealed distinct microbial landscapes. Hierarchical clustering based on microbial transcripts separated samples into two groups predominantly defined by racial ancestry. Bacterial genera including Hafnia and Cedecea were more abundant in AA tumors, while Erwinia was higher in EA tumors. Cellular composition analysis by xCell revealed differences in immune cell populations, with AA tumors having higher Th1 cell abundance and EA tumors containing higher macrophage M2 cell abundance. Nonetheless, AA women with high M2 abundance experienced poorer disease-free survival (DFS) than EA women. Integrative analyses revealed that high expression of human SPDYE2B gene was associated with Hafnia abundance and decreased DFS, highlighting complex host-microbe interactions in TNBC patients.https://doi.org/10.1038/s41523-025-00806-y |
| spellingShingle | Roshan Kumar Susan Duyar-Ayerdi Aishwarya Sundaresan Vinodh Srinivasasainagendra Chandra Sekhar Pedamallu Michael Behring Darshan Shimoga Chandrashekar Isam-Eldin Eltoum Sooryanarayana Varambally Hemant K. Tiwari Sadeep Shrestha Paul L. Auer Lubna N. Chaudhary John R. Kirby Clayton Yates Upender Manne Akinyemi I. Ojesina Race-related host and microbe transcriptomic signatures in triple-negative breast cancer npj Breast Cancer |
| title | Race-related host and microbe transcriptomic signatures in triple-negative breast cancer |
| title_full | Race-related host and microbe transcriptomic signatures in triple-negative breast cancer |
| title_fullStr | Race-related host and microbe transcriptomic signatures in triple-negative breast cancer |
| title_full_unstemmed | Race-related host and microbe transcriptomic signatures in triple-negative breast cancer |
| title_short | Race-related host and microbe transcriptomic signatures in triple-negative breast cancer |
| title_sort | race related host and microbe transcriptomic signatures in triple negative breast cancer |
| url | https://doi.org/10.1038/s41523-025-00806-y |
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