Modulation of Dental Pulp Stem Cell Odontogenesis in a Tunable PEG-Fibrinogen Hydrogel System
Injectable hydrogels have the great potential for clinical translation of dental pulp regeneration. A recently developed PEG-fibrinogen (PF) hydrogel, which comprises a bioactive fibrinogen backbone conjugated to polyethylene glycol (PEG) side chains, can be cross-linked after injection by photopoly...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2015/525367 |
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| author | Qiqi Lu Mirali Pandya Abdul Jalil Rufaihah Vinicius Rosa Huei Jinn Tong Dror Seliktar Wei Seong Toh |
| author_facet | Qiqi Lu Mirali Pandya Abdul Jalil Rufaihah Vinicius Rosa Huei Jinn Tong Dror Seliktar Wei Seong Toh |
| author_sort | Qiqi Lu |
| collection | DOAJ |
| description | Injectable hydrogels have the great potential for clinical translation of dental pulp regeneration. A recently developed PEG-fibrinogen (PF) hydrogel, which comprises a bioactive fibrinogen backbone conjugated to polyethylene glycol (PEG) side chains, can be cross-linked after injection by photopolymerization. The objective of this study was to investigate the use of this hydrogel, which allows tuning of its mechanical properties, as a scaffold for dental pulp tissue engineering. The cross-linking degree of PF hydrogels could be controlled by varying the amounts of PEG-diacrylate (PEG-DA) cross-linker. PF hydrogels are generally cytocompatible with the encapsulated dental pulp stem cells (DPSCs), yielding >85% cell viability in all hydrogels. It was found that the cell morphology of encapsulated DPSCs, odontogenic gene expression, and mineralization were strongly modulated by the hydrogel cross-linking degree and matrix stiffness. Notably, DPSCs cultured within the highest cross-linked hydrogel remained mostly rounded in aggregates and demonstrated the greatest enhancement in odontogenic gene expression. Consistently, the highest degree of mineralization was observed in the highest cross-linked hydrogel. Collectively, our results indicate that PF hydrogels can be used as a scaffold for DPSCs and offers the possibility of influencing DPSCs in ways that may be beneficial for applications in regenerative endodontics. |
| format | Article |
| id | doaj-art-d48591affe2f4db385d10c67d12bb383 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-d48591affe2f4db385d10c67d12bb3832025-02-03T01:00:51ZengWileyStem Cells International1687-966X1687-96782015-01-01201510.1155/2015/525367525367Modulation of Dental Pulp Stem Cell Odontogenesis in a Tunable PEG-Fibrinogen Hydrogel SystemQiqi Lu0Mirali Pandya1Abdul Jalil Rufaihah2Vinicius Rosa3Huei Jinn Tong4Dror Seliktar5Wei Seong Toh6Faculty of Dentistry, National University of Singapore, 11 Lower Kent Ridge Road, 119083, SingaporeFaculty of Dentistry, National University of Singapore, 11 Lower Kent Ridge Road, 119083, SingaporeDepartment of Surgery, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, 1E Kent Ridge Road, 119288, SingaporeFaculty of Dentistry, National University of Singapore, 11 Lower Kent Ridge Road, 119083, SingaporeFaculty of Dentistry, National University of Singapore, 11 Lower Kent Ridge Road, 119083, SingaporeNanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, 2 Engineering Drive 3, 117581, SingaporeFaculty of Dentistry, National University of Singapore, 11 Lower Kent Ridge Road, 119083, SingaporeInjectable hydrogels have the great potential for clinical translation of dental pulp regeneration. A recently developed PEG-fibrinogen (PF) hydrogel, which comprises a bioactive fibrinogen backbone conjugated to polyethylene glycol (PEG) side chains, can be cross-linked after injection by photopolymerization. The objective of this study was to investigate the use of this hydrogel, which allows tuning of its mechanical properties, as a scaffold for dental pulp tissue engineering. The cross-linking degree of PF hydrogels could be controlled by varying the amounts of PEG-diacrylate (PEG-DA) cross-linker. PF hydrogels are generally cytocompatible with the encapsulated dental pulp stem cells (DPSCs), yielding >85% cell viability in all hydrogels. It was found that the cell morphology of encapsulated DPSCs, odontogenic gene expression, and mineralization were strongly modulated by the hydrogel cross-linking degree and matrix stiffness. Notably, DPSCs cultured within the highest cross-linked hydrogel remained mostly rounded in aggregates and demonstrated the greatest enhancement in odontogenic gene expression. Consistently, the highest degree of mineralization was observed in the highest cross-linked hydrogel. Collectively, our results indicate that PF hydrogels can be used as a scaffold for DPSCs and offers the possibility of influencing DPSCs in ways that may be beneficial for applications in regenerative endodontics.http://dx.doi.org/10.1155/2015/525367 |
| spellingShingle | Qiqi Lu Mirali Pandya Abdul Jalil Rufaihah Vinicius Rosa Huei Jinn Tong Dror Seliktar Wei Seong Toh Modulation of Dental Pulp Stem Cell Odontogenesis in a Tunable PEG-Fibrinogen Hydrogel System Stem Cells International |
| title | Modulation of Dental Pulp Stem Cell Odontogenesis in a Tunable PEG-Fibrinogen Hydrogel System |
| title_full | Modulation of Dental Pulp Stem Cell Odontogenesis in a Tunable PEG-Fibrinogen Hydrogel System |
| title_fullStr | Modulation of Dental Pulp Stem Cell Odontogenesis in a Tunable PEG-Fibrinogen Hydrogel System |
| title_full_unstemmed | Modulation of Dental Pulp Stem Cell Odontogenesis in a Tunable PEG-Fibrinogen Hydrogel System |
| title_short | Modulation of Dental Pulp Stem Cell Odontogenesis in a Tunable PEG-Fibrinogen Hydrogel System |
| title_sort | modulation of dental pulp stem cell odontogenesis in a tunable peg fibrinogen hydrogel system |
| url | http://dx.doi.org/10.1155/2015/525367 |
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