Enhanced metabolic adaptations following late dark phase wheel running in high-fat diet-fed mice
Exercise interventions represent an effective strategy to prevent and treat metabolic diseases and the time-of-day-dependent effects of exercise on metabolic outcomes are becoming increasingly apparent. We aimed to study the influence of time-restricted wheel running on whole-body energy and glucose...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-05-01
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| Series: | Molecular Metabolism |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212877825000237 |
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| author | Stephen P. Ashcroft Amy M. Ehrlich Krzysztof Burek Logan A. Pendergrast Caio Y. Yonamine Jonas T. Treebak Juleen R. Zierath |
| author_facet | Stephen P. Ashcroft Amy M. Ehrlich Krzysztof Burek Logan A. Pendergrast Caio Y. Yonamine Jonas T. Treebak Juleen R. Zierath |
| author_sort | Stephen P. Ashcroft |
| collection | DOAJ |
| description | Exercise interventions represent an effective strategy to prevent and treat metabolic diseases and the time-of-day-dependent effects of exercise on metabolic outcomes are becoming increasingly apparent. We aimed to study the influence of time-restricted wheel running on whole-body energy and glucose homeostasis. Male, 8-week-old, C57BL/6NTac mice were fed either a 60% high-fat diet (HFD) or a 10% low-fat diet (LFD) for 4 weeks. Following this, mice were given access to a running wheel between zeitgeber time (ZT) 12–16 (early dark phase) or ZT 20-0 (late dark phase). Sedentary mice had access to a permanently locked wheel. Mice were housed under these conditions in metabolic chambers for 4 weeks in which LFD and HFD conditions were maintained. Following the exercise intervention, body composition and glucose tolerance were assessed. Wheel running during either the early or late dark phase resulted in metabolic improvements such as attenuation in body weight gain, enhanced glucose tolerance and reduced ectopic lipid deposition. However, late dark phase exercise resulted in a greater reduction in body weight gain, as well as enhanced metabolic flexibility and insulin sensitivity. Our data suggest that late dark phase versus early dark phase exercise confers greater metabolic adaptations in HFD-fed mice. |
| format | Article |
| id | doaj-art-d47a0715078c4af1b4f56cfbd0f64edd |
| institution | OA Journals |
| issn | 2212-8778 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Metabolism |
| spelling | doaj-art-d47a0715078c4af1b4f56cfbd0f64edd2025-08-20T02:07:57ZengElsevierMolecular Metabolism2212-87782025-05-019510211610.1016/j.molmet.2025.102116Enhanced metabolic adaptations following late dark phase wheel running in high-fat diet-fed miceStephen P. Ashcroft0Amy M. Ehrlich1Krzysztof Burek2Logan A. Pendergrast3Caio Y. Yonamine4Jonas T. Treebak5Juleen R. Zierath6Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, DenmarkNovo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, DenmarkNovo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, DenmarkIntegrative Physiology Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, SwedenNovo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, DenmarkNovo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, DenmarkNovo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark; Integrative Physiology Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden; Integrative Physiology Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden; Corresponding author. Department of Molecular Medicine and Surgery, Section of Integrative Physiology, Biomedicum, Solnavägen 9, SE-171 77 Stockholm, Sweden.Exercise interventions represent an effective strategy to prevent and treat metabolic diseases and the time-of-day-dependent effects of exercise on metabolic outcomes are becoming increasingly apparent. We aimed to study the influence of time-restricted wheel running on whole-body energy and glucose homeostasis. Male, 8-week-old, C57BL/6NTac mice were fed either a 60% high-fat diet (HFD) or a 10% low-fat diet (LFD) for 4 weeks. Following this, mice were given access to a running wheel between zeitgeber time (ZT) 12–16 (early dark phase) or ZT 20-0 (late dark phase). Sedentary mice had access to a permanently locked wheel. Mice were housed under these conditions in metabolic chambers for 4 weeks in which LFD and HFD conditions were maintained. Following the exercise intervention, body composition and glucose tolerance were assessed. Wheel running during either the early or late dark phase resulted in metabolic improvements such as attenuation in body weight gain, enhanced glucose tolerance and reduced ectopic lipid deposition. However, late dark phase exercise resulted in a greater reduction in body weight gain, as well as enhanced metabolic flexibility and insulin sensitivity. Our data suggest that late dark phase versus early dark phase exercise confers greater metabolic adaptations in HFD-fed mice.http://www.sciencedirect.com/science/article/pii/S2212877825000237ExerciseMetabolismCircadian |
| spellingShingle | Stephen P. Ashcroft Amy M. Ehrlich Krzysztof Burek Logan A. Pendergrast Caio Y. Yonamine Jonas T. Treebak Juleen R. Zierath Enhanced metabolic adaptations following late dark phase wheel running in high-fat diet-fed mice Molecular Metabolism Exercise Metabolism Circadian |
| title | Enhanced metabolic adaptations following late dark phase wheel running in high-fat diet-fed mice |
| title_full | Enhanced metabolic adaptations following late dark phase wheel running in high-fat diet-fed mice |
| title_fullStr | Enhanced metabolic adaptations following late dark phase wheel running in high-fat diet-fed mice |
| title_full_unstemmed | Enhanced metabolic adaptations following late dark phase wheel running in high-fat diet-fed mice |
| title_short | Enhanced metabolic adaptations following late dark phase wheel running in high-fat diet-fed mice |
| title_sort | enhanced metabolic adaptations following late dark phase wheel running in high fat diet fed mice |
| topic | Exercise Metabolism Circadian |
| url | http://www.sciencedirect.com/science/article/pii/S2212877825000237 |
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