PGC-1 alpha overexpression in the skeletal muscle results in a metabolically active microbiome which is independent of redox signaling
Abstract In this study, we investigated the potential relationship between the mitochondrial network and the microbiome using wild-type and skeletal muscle-specific PGC-1α (Pparg coactivator 1 alpha) overexpressing mice, both with and without exercise training. Basal PGC-1α levels were significantly...
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Nature Portfolio
2025-07-01
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| Online Access: | https://doi.org/10.1038/s41598-025-05594-w |
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| author | Erika Koltai Soroosh Mozaffaritabar Lei Zhou Attila Kolonics Atsuko Koike Kumpei Tanisawa Jonguk Park Ferenc Torma Zsolt Radak |
| author_facet | Erika Koltai Soroosh Mozaffaritabar Lei Zhou Attila Kolonics Atsuko Koike Kumpei Tanisawa Jonguk Park Ferenc Torma Zsolt Radak |
| author_sort | Erika Koltai |
| collection | DOAJ |
| description | Abstract In this study, we investigated the potential relationship between the mitochondrial network and the microbiome using wild-type and skeletal muscle-specific PGC-1α (Pparg coactivator 1 alpha) overexpressing mice, both with and without exercise training. Basal PGC-1α levels were significantly higher in the skeletal muscle (J Physiol Biochem 80:329–335, 2024. https://doi.org/10.1007/s13105-024-01006-1 ) and, notably, in the colon, which is anatomically proximal to the microbiome. However, no significant changes were observed in cell signaling or mitochondria-related proteins within the colon. On the other hand, mitochondrial H₂O₂ production in the colon decreased in the PGC-1α overexpressing group. The relative abundance of several bacterial taxa differed between wild-type and PGC-1α overexpressing groups at baseline condition, indicating a shift in the microbiome milieu probably to cope with the increased metabolism, enhanced short-chain fatty acid utilization, and improved endurance capacity. Ten weeks of exercise training differentially modulated the host microbiome in PGC-1α overexpressing and wild-type mice, facilitating adaptations to a broad range of exercise-induced challenges. The results of this study provide new insights into the possible cross-talk between mitochondria and the microbiome. |
| format | Article |
| id | doaj-art-d479207ea9ff4fd8b09b80138018aeae |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-d479207ea9ff4fd8b09b80138018aeae2025-08-20T03:45:25ZengNature PortfolioScientific Reports2045-23222025-07-011511910.1038/s41598-025-05594-wPGC-1 alpha overexpression in the skeletal muscle results in a metabolically active microbiome which is independent of redox signalingErika Koltai0Soroosh Mozaffaritabar1Lei Zhou2Attila Kolonics3Atsuko Koike4Kumpei Tanisawa5Jonguk Park6Ferenc Torma7Zsolt Radak8Research Institute of Sport Science, Hungarian University of Sport ScienceResearch Institute of Sport Science, Hungarian University of Sport ScienceResearch Institute of Sport Science, Hungarian University of Sport ScienceResearch Institute of Sport Science, Hungarian University of Sport ScienceResearch Institute of Sport Science, Hungarian University of Sport ScienceFaculty of Sport Sciences, Waseda UniversityArtificial Intelligence Center for Health and Biomedical ResearchResearch Institute of Sport Science, Hungarian University of Sport ScienceResearch Institute of Sport Science, Hungarian University of Sport ScienceAbstract In this study, we investigated the potential relationship between the mitochondrial network and the microbiome using wild-type and skeletal muscle-specific PGC-1α (Pparg coactivator 1 alpha) overexpressing mice, both with and without exercise training. Basal PGC-1α levels were significantly higher in the skeletal muscle (J Physiol Biochem 80:329–335, 2024. https://doi.org/10.1007/s13105-024-01006-1 ) and, notably, in the colon, which is anatomically proximal to the microbiome. However, no significant changes were observed in cell signaling or mitochondria-related proteins within the colon. On the other hand, mitochondrial H₂O₂ production in the colon decreased in the PGC-1α overexpressing group. The relative abundance of several bacterial taxa differed between wild-type and PGC-1α overexpressing groups at baseline condition, indicating a shift in the microbiome milieu probably to cope with the increased metabolism, enhanced short-chain fatty acid utilization, and improved endurance capacity. Ten weeks of exercise training differentially modulated the host microbiome in PGC-1α overexpressing and wild-type mice, facilitating adaptations to a broad range of exercise-induced challenges. The results of this study provide new insights into the possible cross-talk between mitochondria and the microbiome.https://doi.org/10.1038/s41598-025-05594-wMitochondriaMicrobiotaPGC-1αPhysical exerciseHost–microbial interaction |
| spellingShingle | Erika Koltai Soroosh Mozaffaritabar Lei Zhou Attila Kolonics Atsuko Koike Kumpei Tanisawa Jonguk Park Ferenc Torma Zsolt Radak PGC-1 alpha overexpression in the skeletal muscle results in a metabolically active microbiome which is independent of redox signaling Scientific Reports Mitochondria Microbiota PGC-1α Physical exercise Host–microbial interaction |
| title | PGC-1 alpha overexpression in the skeletal muscle results in a metabolically active microbiome which is independent of redox signaling |
| title_full | PGC-1 alpha overexpression in the skeletal muscle results in a metabolically active microbiome which is independent of redox signaling |
| title_fullStr | PGC-1 alpha overexpression in the skeletal muscle results in a metabolically active microbiome which is independent of redox signaling |
| title_full_unstemmed | PGC-1 alpha overexpression in the skeletal muscle results in a metabolically active microbiome which is independent of redox signaling |
| title_short | PGC-1 alpha overexpression in the skeletal muscle results in a metabolically active microbiome which is independent of redox signaling |
| title_sort | pgc 1 alpha overexpression in the skeletal muscle results in a metabolically active microbiome which is independent of redox signaling |
| topic | Mitochondria Microbiota PGC-1α Physical exercise Host–microbial interaction |
| url | https://doi.org/10.1038/s41598-025-05594-w |
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