Seroprevalence of hepatitis E in pigs and wild boars in the region of the city Belgrade

Introduction: Hepatitis E is considered an emerging human viral disease with many evidences of zoonotic nature of disease, and swine are the main reservoir of HEV. The aim of this study was to determine HEV seroprevalence in commercial pig farms, backyard pigs, slaughtered pigs and wild boars in th...

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Main Authors: Branislav Kureljušić, Božidar Savić, Nemanja Jezdimirović, Jasna Kureljušić, Vesna Milićević, Neđeljko Karabasil, Slavica Vesković Moračanin, Jadranka Žutić
Format: Article
Language:English
Published: The Journal of Infection in Developing Countries 2020-06-01
Series:Journal of Infection in Developing Countries
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Online Access:https://jidc.org/index.php/journal/article/view/12552
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author Branislav Kureljušić
Božidar Savić
Nemanja Jezdimirović
Jasna Kureljušić
Vesna Milićević
Neđeljko Karabasil
Slavica Vesković Moračanin
Jadranka Žutić
author_facet Branislav Kureljušić
Božidar Savić
Nemanja Jezdimirović
Jasna Kureljušić
Vesna Milićević
Neđeljko Karabasil
Slavica Vesković Moračanin
Jadranka Žutić
author_sort Branislav Kureljušić
collection DOAJ
description Introduction: Hepatitis E is considered an emerging human viral disease with many evidences of zoonotic nature of disease, and swine are the main reservoir of HEV. The aim of this study was to determine HEV seroprevalence in commercial pig farms, backyard pigs, slaughtered pigs and wild boars in the region of the city Belgrade. Methodology: A total of 405 sera samples: 150 samples from 3 commercial pig farms, 70 samples from backyard pigs, 119 samples from slaughtered pigs and 66 samples from wild boars of the region of the city Belgrade, Serbia were analysed by commercial ELISA test. Results: The overall HEV seroprevalence in 3 commercial pig farms was 55.33% (83/150). All tested farms (farm A, B and C) were positive on the presence of anti-HEV antibodies, respectively 58% (29/50), 54% (27/50) and 54% (27/50). From 70 tested backyard pigs, 75.71% (53/70) were tested seropositive. In total, 26 backyard pig holidngs were confirmed as positive to anti-HEV antibodies (81.25%). At slaughterhouse, 25% (8/32) weaned piglets and 20.69% (18/87) fattening pigs were tested positive on anti-HEV antibodies. Overall HEV seroprevalence in tested wild boar population was 52.25% (36/66). Conclusions: Detected very high seroprevalence of anti-HEV antibodies indicated an active circulation of HEV, being enzootic in the swine population, and wild boars, as well, in the region of the city Belgrade.
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spelling doaj-art-d4766d5473ca431d94e115ea5bacadd82025-08-20T02:57:44ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802020-06-01140610.3855/jidc.12552Seroprevalence of hepatitis E in pigs and wild boars in the region of the city BelgradeBranislav Kureljušić0Božidar Savić1Nemanja Jezdimirović2Jasna Kureljušić3Vesna Milićević4Neđeljko Karabasil5Slavica Vesković Moračanin6Jadranka Žutić7Department of Pathology, Institute of Veterinary Medicine of Serbia, Belgrade, SerbiaDepartment of Pathology, Institute of Veterinary Medicine of Serbia, Belgrade, SerbiaDepartment of Pathology, Institute of Veterinary Medicine of Serbia, Belgrade, SerbiaDepartment of Food and Feed Safety, Institute of Veterinary Medicine of Serbia, Belgrade, SerbiaDepartment of Virology, Institute of Veterinary Medicine of Serbia, Belgrade, SerbiaDepartment of Food Hygiene and Technology of Animal Origin, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, SerbiaSector for Development and Technology Transfer, Institute of Meat Hygiene and Technology, Belgrade, SerbiaDepartment of Pathology, Institute of Veterinary Medicine of Serbia, Belgrade, Serbia Introduction: Hepatitis E is considered an emerging human viral disease with many evidences of zoonotic nature of disease, and swine are the main reservoir of HEV. The aim of this study was to determine HEV seroprevalence in commercial pig farms, backyard pigs, slaughtered pigs and wild boars in the region of the city Belgrade. Methodology: A total of 405 sera samples: 150 samples from 3 commercial pig farms, 70 samples from backyard pigs, 119 samples from slaughtered pigs and 66 samples from wild boars of the region of the city Belgrade, Serbia were analysed by commercial ELISA test. Results: The overall HEV seroprevalence in 3 commercial pig farms was 55.33% (83/150). All tested farms (farm A, B and C) were positive on the presence of anti-HEV antibodies, respectively 58% (29/50), 54% (27/50) and 54% (27/50). From 70 tested backyard pigs, 75.71% (53/70) were tested seropositive. In total, 26 backyard pig holidngs were confirmed as positive to anti-HEV antibodies (81.25%). At slaughterhouse, 25% (8/32) weaned piglets and 20.69% (18/87) fattening pigs were tested positive on anti-HEV antibodies. Overall HEV seroprevalence in tested wild boar population was 52.25% (36/66). Conclusions: Detected very high seroprevalence of anti-HEV antibodies indicated an active circulation of HEV, being enzootic in the swine population, and wild boars, as well, in the region of the city Belgrade. https://jidc.org/index.php/journal/article/view/12552hepatitis EpigELISAprevalence, farmfarmslaughterhouse
spellingShingle Branislav Kureljušić
Božidar Savić
Nemanja Jezdimirović
Jasna Kureljušić
Vesna Milićević
Neđeljko Karabasil
Slavica Vesković Moračanin
Jadranka Žutić
Seroprevalence of hepatitis E in pigs and wild boars in the region of the city Belgrade
Journal of Infection in Developing Countries
hepatitis E
pig
ELISA
prevalence, farm
farm
slaughterhouse
title Seroprevalence of hepatitis E in pigs and wild boars in the region of the city Belgrade
title_full Seroprevalence of hepatitis E in pigs and wild boars in the region of the city Belgrade
title_fullStr Seroprevalence of hepatitis E in pigs and wild boars in the region of the city Belgrade
title_full_unstemmed Seroprevalence of hepatitis E in pigs and wild boars in the region of the city Belgrade
title_short Seroprevalence of hepatitis E in pigs and wild boars in the region of the city Belgrade
title_sort seroprevalence of hepatitis e in pigs and wild boars in the region of the city belgrade
topic hepatitis E
pig
ELISA
prevalence, farm
farm
slaughterhouse
url https://jidc.org/index.php/journal/article/view/12552
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