Prebiotic Effect of Oxidized Hydroxypropyl Starch via In Vitro and In Vivo
Most studies on resistant starch are limited to its effect on blood glucose; there are few studies on the prebiotic effects of resistant starch on the gut. In this experiment, through in vivo metabolism verification and in vitro simulated fermentation experiments, it was found that hydroxypropyl oxi...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/14/13/2217 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850118190159364096 |
|---|---|
| author | Huiwen Zheng Zhipu Xu Yiwen Fan Jiazhi Han Liyang Zhou Han Li Xiaohua Pan Rongrong Ma Chang Liu Yaoqi Tian |
| author_facet | Huiwen Zheng Zhipu Xu Yiwen Fan Jiazhi Han Liyang Zhou Han Li Xiaohua Pan Rongrong Ma Chang Liu Yaoqi Tian |
| author_sort | Huiwen Zheng |
| collection | DOAJ |
| description | Most studies on resistant starch are limited to its effect on blood glucose; there are few studies on the prebiotic effects of resistant starch on the gut. In this experiment, through in vivo metabolism verification and in vitro simulated fermentation experiments, it was found that hydroxypropyl oxide (OHS) had a prebiotic effect on the intestine. The results of bioinformatics showed that the structure of the microbiota changed significantly, and the in vitro and in vivo fermentation results of <i>Bacteroides uniformis</i> and <i>Parabacteroides distasonis</i> showed an upward trend. The results of a KEGG prediction of the metabolic pathway showed that Phenylalanine metabolism and Cysteine and methionine metabolism showed an enhanced trend. At the same time, the results of in vitro and in vivo metabolite assays further confirmed this point, and the content of L-Homocystine and Phenylalanine in metabolites decreased significantly, with the decrease in L-Homocystine posing a reduction in cardiovascular disease risk and the decrease in Phenylalanine having a positive significance for phenylketonuria patients. This study proved that hydroxypropyl oxide can regulate the intestinal microbiota and has intestinal prebiotic effects, which can be used to guide the development of functional foods. |
| format | Article |
| id | doaj-art-d4764a1e745a43f8a4775fe5dd54ee00 |
| institution | OA Journals |
| issn | 2304-8158 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj-art-d4764a1e745a43f8a4775fe5dd54ee002025-08-20T02:35:56ZengMDPI AGFoods2304-81582025-06-011413221710.3390/foods14132217Prebiotic Effect of Oxidized Hydroxypropyl Starch via In Vitro and In VivoHuiwen Zheng0Zhipu Xu1Yiwen Fan2Jiazhi Han3Liyang Zhou4Han Li5Xiaohua Pan6Rongrong Ma7Chang Liu8Yaoqi Tian9State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, ChinaMost studies on resistant starch are limited to its effect on blood glucose; there are few studies on the prebiotic effects of resistant starch on the gut. In this experiment, through in vivo metabolism verification and in vitro simulated fermentation experiments, it was found that hydroxypropyl oxide (OHS) had a prebiotic effect on the intestine. The results of bioinformatics showed that the structure of the microbiota changed significantly, and the in vitro and in vivo fermentation results of <i>Bacteroides uniformis</i> and <i>Parabacteroides distasonis</i> showed an upward trend. The results of a KEGG prediction of the metabolic pathway showed that Phenylalanine metabolism and Cysteine and methionine metabolism showed an enhanced trend. At the same time, the results of in vitro and in vivo metabolite assays further confirmed this point, and the content of L-Homocystine and Phenylalanine in metabolites decreased significantly, with the decrease in L-Homocystine posing a reduction in cardiovascular disease risk and the decrease in Phenylalanine having a positive significance for phenylketonuria patients. This study proved that hydroxypropyl oxide can regulate the intestinal microbiota and has intestinal prebiotic effects, which can be used to guide the development of functional foods.https://www.mdpi.com/2304-8158/14/13/2217oxidized hydroxypropyl starchdigestionfermentgut probiotics |
| spellingShingle | Huiwen Zheng Zhipu Xu Yiwen Fan Jiazhi Han Liyang Zhou Han Li Xiaohua Pan Rongrong Ma Chang Liu Yaoqi Tian Prebiotic Effect of Oxidized Hydroxypropyl Starch via In Vitro and In Vivo Foods oxidized hydroxypropyl starch digestion ferment gut probiotics |
| title | Prebiotic Effect of Oxidized Hydroxypropyl Starch via In Vitro and In Vivo |
| title_full | Prebiotic Effect of Oxidized Hydroxypropyl Starch via In Vitro and In Vivo |
| title_fullStr | Prebiotic Effect of Oxidized Hydroxypropyl Starch via In Vitro and In Vivo |
| title_full_unstemmed | Prebiotic Effect of Oxidized Hydroxypropyl Starch via In Vitro and In Vivo |
| title_short | Prebiotic Effect of Oxidized Hydroxypropyl Starch via In Vitro and In Vivo |
| title_sort | prebiotic effect of oxidized hydroxypropyl starch via in vitro and in vivo |
| topic | oxidized hydroxypropyl starch digestion ferment gut probiotics |
| url | https://www.mdpi.com/2304-8158/14/13/2217 |
| work_keys_str_mv | AT huiwenzheng prebioticeffectofoxidizedhydroxypropylstarchviainvitroandinvivo AT zhipuxu prebioticeffectofoxidizedhydroxypropylstarchviainvitroandinvivo AT yiwenfan prebioticeffectofoxidizedhydroxypropylstarchviainvitroandinvivo AT jiazhihan prebioticeffectofoxidizedhydroxypropylstarchviainvitroandinvivo AT liyangzhou prebioticeffectofoxidizedhydroxypropylstarchviainvitroandinvivo AT hanli prebioticeffectofoxidizedhydroxypropylstarchviainvitroandinvivo AT xiaohuapan prebioticeffectofoxidizedhydroxypropylstarchviainvitroandinvivo AT rongrongma prebioticeffectofoxidizedhydroxypropylstarchviainvitroandinvivo AT changliu prebioticeffectofoxidizedhydroxypropylstarchviainvitroandinvivo AT yaoqitian prebioticeffectofoxidizedhydroxypropylstarchviainvitroandinvivo |