Mapping hard tissue maturation: the role of Gallego stain in differential staining
Histological stains are specific dyes that attach to different tissues and are used in conjunction with hematoxylin and eosin stains in routine histopathology to provide useful information on tissues. With the usual stains, it becomes difficult to distinguish between the different hard tissues prese...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-07-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426825001198 |
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| author | Shweta Mary Reddy Suganya Panneer Selvam Ramya Ramadoss Sandhya Sundar |
| author_facet | Shweta Mary Reddy Suganya Panneer Selvam Ramya Ramadoss Sandhya Sundar |
| author_sort | Shweta Mary Reddy |
| collection | DOAJ |
| description | Histological stains are specific dyes that attach to different tissues and are used in conjunction with hematoxylin and eosin stains in routine histopathology to provide useful information on tissues. With the usual stains, it becomes difficult to distinguish between the different hard tissues present in oral pathological disorders. As one of the differential stains to discriminate between the hard tissues, modified Gallego's stain can be utilized. Maxilla and mandible of a 3-day-old rabbit are collected, followed by processing and embedding of the tissues, thin paraffin sections were made & stained with Modified Gallego's, and then viewed under the microscope. In the developing tooth germ, Dentin is stained green, and enamel is stained red in Modified Gallego staining. Modified Gallego staining facilitated the identification of the characteristics of hard and soft tissues during development and the changes in their behavior during maturation. |
| format | Article |
| id | doaj-art-d4761b315d584dcba479bc4abbeb464d |
| institution | DOAJ |
| issn | 2212-4268 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-d4761b315d584dcba479bc4abbeb464d2025-08-20T03:23:29ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682025-07-0115485886310.1016/j.jobcr.2025.06.004Mapping hard tissue maturation: the role of Gallego stain in differential stainingShweta Mary Reddy0Suganya Panneer Selvam1Ramya Ramadoss2Sandhya Sundar3Department of Oral Biology, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Chennai, 600077, IndiaCorresponding author. Department of Oral Biology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, India.; Department of Oral Biology, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Chennai, 600077, IndiaDepartment of Oral Biology, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Chennai, 600077, IndiaDepartment of Oral Biology, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Chennai, 600077, IndiaHistological stains are specific dyes that attach to different tissues and are used in conjunction with hematoxylin and eosin stains in routine histopathology to provide useful information on tissues. With the usual stains, it becomes difficult to distinguish between the different hard tissues present in oral pathological disorders. As one of the differential stains to discriminate between the hard tissues, modified Gallego's stain can be utilized. Maxilla and mandible of a 3-day-old rabbit are collected, followed by processing and embedding of the tissues, thin paraffin sections were made & stained with Modified Gallego's, and then viewed under the microscope. In the developing tooth germ, Dentin is stained green, and enamel is stained red in Modified Gallego staining. Modified Gallego staining facilitated the identification of the characteristics of hard and soft tissues during development and the changes in their behavior during maturation.http://www.sciencedirect.com/science/article/pii/S2212426825001198BoneCartilageCollagenDentinEnamelTooth germ |
| spellingShingle | Shweta Mary Reddy Suganya Panneer Selvam Ramya Ramadoss Sandhya Sundar Mapping hard tissue maturation: the role of Gallego stain in differential staining Journal of Oral Biology and Craniofacial Research Bone Cartilage Collagen Dentin Enamel Tooth germ |
| title | Mapping hard tissue maturation: the role of Gallego stain in differential staining |
| title_full | Mapping hard tissue maturation: the role of Gallego stain in differential staining |
| title_fullStr | Mapping hard tissue maturation: the role of Gallego stain in differential staining |
| title_full_unstemmed | Mapping hard tissue maturation: the role of Gallego stain in differential staining |
| title_short | Mapping hard tissue maturation: the role of Gallego stain in differential staining |
| title_sort | mapping hard tissue maturation the role of gallego stain in differential staining |
| topic | Bone Cartilage Collagen Dentin Enamel Tooth germ |
| url | http://www.sciencedirect.com/science/article/pii/S2212426825001198 |
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