Dynamics of Nitrogen Mineralization by Organic and Inorganic Amendments Through Enzyme Activity of Microbial Community in Laboratory Incubation

Chemical fertilizers provide an immediate nitrogen supply but require repeated application at critical growth stages; however, excessive chemical fertilizer application harms the environment. In contrast, organic fertilizers release nitrogen gradually for a long time, and microbial fertilizers enhan...

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Main Authors: Md.Tariful Alam Khan, Md. Billal Hossain Momen, Md. Rashedur Rahman Tanvir, Md. Robiul Islam
Format: Article
Language:English
Published: Universitas Sebelas Maret, Faculty of Agriculture 2025-03-01
Series:Caraka Tani: Journal of Sustainable Agriculture
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Online Access:https://jurnal.uns.ac.id/carakatani/article/view/92849
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author Md.Tariful Alam Khan
Md. Billal Hossain Momen
Md. Rashedur Rahman Tanvir
Md. Robiul Islam
author_facet Md.Tariful Alam Khan
Md. Billal Hossain Momen
Md. Rashedur Rahman Tanvir
Md. Robiul Islam
author_sort Md.Tariful Alam Khan
collection DOAJ
description Chemical fertilizers provide an immediate nitrogen supply but require repeated application at critical growth stages; however, excessive chemical fertilizer application harms the environment. In contrast, organic fertilizers release nitrogen gradually for a long time, and microbial fertilizers enhance nutrient availability. This study investigated the effects of integrating chemical nitrogen (CN), poultry manure (PM), and microbial fertilizer (MBF) on soil nitrogen availability and microbial activity. Eight treatments were applied: T0 (control), T1 (100% CN), T2 (100% CN + MBF), T3 (75% CN + 25% PM + MBF), T4 (50% CN + 50% PM + MBF), T5 (25% CN + 75% PM + MBF), T6 (100% PM + MBF), and T7 (100% PM). Soil nitrogen fractions, microbial biomass, enzyme activities, and phospholipid fatty acid (PLFA) composition were analyzed. Integrated treatments improved nitrogen availability compared to sole CN application, with T4 showing the highest NO₃--N accumulation. Additionally, T4 increased total nitrogen, organic carbon, and microbial biomass, enhancing soil fertility. Enzymatic activities, including urease, catalase, invertase, and cellulase, responded positively to the integrated treatments, reflecting improved soil health. PLFA analysis revealed shifts in microbial community composition, highlighting the role of PM in promoting microbial diversity and biomass. These findings highlight that blending 50% CN and 50% PM with MBF balances immediate and sustained nitrogen release while stimulating microbial diversity and soil enzyme functions and improves overall soil health, making it a promising strategy for sustainable soil fertility management and reducing chemical fertilizer dependency.
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publisher Universitas Sebelas Maret, Faculty of Agriculture
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spelling doaj-art-d46b2d9be2ce4adfb8f6203a242a3cfa2025-08-20T03:18:05ZengUniversitas Sebelas Maret, Faculty of AgricultureCaraka Tani: Journal of Sustainable Agriculture2613-94562599-25702025-03-0140223125010.20961/carakatani.v40i2.9284945230Dynamics of Nitrogen Mineralization by Organic and Inorganic Amendments Through Enzyme Activity of Microbial Community in Laboratory IncubationMd.Tariful Alam Khan0Md. Billal Hossain Momen1Md. Rashedur Rahman Tanvir2Md. Robiul Islam3Farming Systems Engineering Laboratory, Department of Agronomy and Agricultural Extension, University of Rajshahi, RajshahiFarming Systems Engineering Laboratory, Department of Agronomy and Agricultural Extension, University of Rajshahi, RajshahiFarming Systems Engineering Laboratory, Department of Agronomy and Agricultural Extension, University of Rajshahi, RajshahiFarming Systems Engineering Laboratory, Department of Agronomy and Agricultural Extension, University of Rajshahi, RajshahiChemical fertilizers provide an immediate nitrogen supply but require repeated application at critical growth stages; however, excessive chemical fertilizer application harms the environment. In contrast, organic fertilizers release nitrogen gradually for a long time, and microbial fertilizers enhance nutrient availability. This study investigated the effects of integrating chemical nitrogen (CN), poultry manure (PM), and microbial fertilizer (MBF) on soil nitrogen availability and microbial activity. Eight treatments were applied: T0 (control), T1 (100% CN), T2 (100% CN + MBF), T3 (75% CN + 25% PM + MBF), T4 (50% CN + 50% PM + MBF), T5 (25% CN + 75% PM + MBF), T6 (100% PM + MBF), and T7 (100% PM). Soil nitrogen fractions, microbial biomass, enzyme activities, and phospholipid fatty acid (PLFA) composition were analyzed. Integrated treatments improved nitrogen availability compared to sole CN application, with T4 showing the highest NO₃--N accumulation. Additionally, T4 increased total nitrogen, organic carbon, and microbial biomass, enhancing soil fertility. Enzymatic activities, including urease, catalase, invertase, and cellulase, responded positively to the integrated treatments, reflecting improved soil health. PLFA analysis revealed shifts in microbial community composition, highlighting the role of PM in promoting microbial diversity and biomass. These findings highlight that blending 50% CN and 50% PM with MBF balances immediate and sustained nitrogen release while stimulating microbial diversity and soil enzyme functions and improves overall soil health, making it a promising strategy for sustainable soil fertility management and reducing chemical fertilizer dependency.https://jurnal.uns.ac.id/carakatani/article/view/92849integrated fertilizermicrobial fertilizermineralizationplfaspoultry manure
spellingShingle Md.Tariful Alam Khan
Md. Billal Hossain Momen
Md. Rashedur Rahman Tanvir
Md. Robiul Islam
Dynamics of Nitrogen Mineralization by Organic and Inorganic Amendments Through Enzyme Activity of Microbial Community in Laboratory Incubation
Caraka Tani: Journal of Sustainable Agriculture
integrated fertilizer
microbial fertilizer
mineralization
plfas
poultry manure
title Dynamics of Nitrogen Mineralization by Organic and Inorganic Amendments Through Enzyme Activity of Microbial Community in Laboratory Incubation
title_full Dynamics of Nitrogen Mineralization by Organic and Inorganic Amendments Through Enzyme Activity of Microbial Community in Laboratory Incubation
title_fullStr Dynamics of Nitrogen Mineralization by Organic and Inorganic Amendments Through Enzyme Activity of Microbial Community in Laboratory Incubation
title_full_unstemmed Dynamics of Nitrogen Mineralization by Organic and Inorganic Amendments Through Enzyme Activity of Microbial Community in Laboratory Incubation
title_short Dynamics of Nitrogen Mineralization by Organic and Inorganic Amendments Through Enzyme Activity of Microbial Community in Laboratory Incubation
title_sort dynamics of nitrogen mineralization by organic and inorganic amendments through enzyme activity of microbial community in laboratory incubation
topic integrated fertilizer
microbial fertilizer
mineralization
plfas
poultry manure
url https://jurnal.uns.ac.id/carakatani/article/view/92849
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AT mdrashedurrahmantanvir dynamicsofnitrogenmineralizationbyorganicandinorganicamendmentsthroughenzymeactivityofmicrobialcommunityinlaboratoryincubation
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