BACTERIOCINS OF Lactobacillus LF221 STRAIN
LF221 strain is a Lactobacillus isolate from a baby’s faeces that was previously found to produce bacteriocin(s) with inhibitory activity against a wide range of bacterial species including some pathogenic and food-spoilage (Bacillus cereus, Clostridium sp., Listeria innocua, Staphylococcus aureus)....
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
1998-12-01
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| Series: | Acta Agriculturae Slovenica |
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| Online Access: | https://journals.uni-lj.si/aas/article/view/17081 |
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| author | Bojana BOGOVIČ MATIJAŠIĆ Irena ROGELJ |
| author_facet | Bojana BOGOVIČ MATIJAŠIĆ Irena ROGELJ |
| author_sort | Bojana BOGOVIČ MATIJAŠIĆ |
| collection | DOAJ |
| description | LF221 strain is a Lactobacillus isolate from a baby’s faeces that was previously found to produce bacteriocin(s) with inhibitory activity against a wide range of bacterial species including some pathogenic and food-spoilage (Bacillus cereus, Clostridium sp., Listeria innocua, Staphylococcus aureus). Isoelectric point and molecular mass of bacteriocin complex and subunits were estimated. Precipitation with ammonium sulphate, cation exchange chromatography, C8 hydrophobic interaction chromatography and reverse-phase FPLC chromatography were used to isolate bacteriocins from the supernatant of MRS culture. Amino acid composition and partial N-terminal amino acid sequences of two isolated bacteriocins were determined. Bacteriocins were present in MRS supernatant as aggregates with Mw>150.000 and pI 5.1-5.3, while Mw of subunits was 3500-5000. A high content of glycin and alanin was observed for both bacteriocins. The N-terminal amino acid sequences showed little similarity with those of other bacteriocins. Acidocins LF221 A and B appear to belong to the II class of LAB bacteriocins. |
| format | Article |
| id | doaj-art-d462b3edfcd3402f9a77c9f43209c0c6 |
| institution | DOAJ |
| issn | 1854-1941 |
| language | English |
| publishDate | 1998-12-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj-art-d462b3edfcd3402f9a77c9f43209c0c62025-08-20T02:57:37ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19411998-12-0172111312110.14720/aas.1998.72.1.1708123475BACTERIOCINS OF Lactobacillus LF221 STRAINBojana BOGOVIČ MATIJAŠIĆ0Irena ROGELJ1Univ. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, SloveniaUniv. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Groblje 3, SI-1230 Domžale, SloveniaLF221 strain is a Lactobacillus isolate from a baby’s faeces that was previously found to produce bacteriocin(s) with inhibitory activity against a wide range of bacterial species including some pathogenic and food-spoilage (Bacillus cereus, Clostridium sp., Listeria innocua, Staphylococcus aureus). Isoelectric point and molecular mass of bacteriocin complex and subunits were estimated. Precipitation with ammonium sulphate, cation exchange chromatography, C8 hydrophobic interaction chromatography and reverse-phase FPLC chromatography were used to isolate bacteriocins from the supernatant of MRS culture. Amino acid composition and partial N-terminal amino acid sequences of two isolated bacteriocins were determined. Bacteriocins were present in MRS supernatant as aggregates with Mw>150.000 and pI 5.1-5.3, while Mw of subunits was 3500-5000. A high content of glycin and alanin was observed for both bacteriocins. The N-terminal amino acid sequences showed little similarity with those of other bacteriocins. Acidocins LF221 A and B appear to belong to the II class of LAB bacteriocins.https://journals.uni-lj.si/aas/article/view/17081microbiologybacterialactobacillusbacteriocinsclassification |
| spellingShingle | Bojana BOGOVIČ MATIJAŠIĆ Irena ROGELJ BACTERIOCINS OF Lactobacillus LF221 STRAIN Acta Agriculturae Slovenica microbiology bacteria lactobacillus bacteriocins classification |
| title | BACTERIOCINS OF Lactobacillus LF221 STRAIN |
| title_full | BACTERIOCINS OF Lactobacillus LF221 STRAIN |
| title_fullStr | BACTERIOCINS OF Lactobacillus LF221 STRAIN |
| title_full_unstemmed | BACTERIOCINS OF Lactobacillus LF221 STRAIN |
| title_short | BACTERIOCINS OF Lactobacillus LF221 STRAIN |
| title_sort | bacteriocins of lactobacillus lf221 strain |
| topic | microbiology bacteria lactobacillus bacteriocins classification |
| url | https://journals.uni-lj.si/aas/article/view/17081 |
| work_keys_str_mv | AT bojanabogovicmatijasic bacteriocinsoflactobacilluslf221strain AT irenarogelj bacteriocinsoflactobacilluslf221strain |