Let-7f-5p Modulates Lipid Metabolism by Targeting Sterol Regulatory Element-Binding Protein 2 in Response to PRRSV Infection
Porcine reproductive and respiratory syndrome (PRRS) has caused substantial damage to the pig industry. MicroRNAs (miRNAs) were found to play crucial roles in modulating the pathogenesis of PRRS virus (PRRSV). In the present study, we revealed that PRRSV induced let-7f-5p to influence lipid metaboli...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-08-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/11/9/392 |
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| author | Dongfeng Jiang Liyu Yang Xiangge Meng Qiuliang Xu Xiang Zhou Bang Liu |
| author_facet | Dongfeng Jiang Liyu Yang Xiangge Meng Qiuliang Xu Xiang Zhou Bang Liu |
| author_sort | Dongfeng Jiang |
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| description | Porcine reproductive and respiratory syndrome (PRRS) has caused substantial damage to the pig industry. MicroRNAs (miRNAs) were found to play crucial roles in modulating the pathogenesis of PRRS virus (PRRSV). In the present study, we revealed that PRRSV induced let-7f-5p to influence lipid metabolism to regulate PRRSV pathogenesis. A transcriptome analysis of PRRSV-infected PK15<sup>CD163</sup> cells transfected with let-7f-5p mimics or negative control (NC) generated 1718 differentially expressed genes, which were primarily associated with lipid metabolism processes. Furthermore, the master regulator of lipogenesis SREBP2 was found to be directly targeted by let-7f-5p using a dual-luciferase reporter system and Western blotting. The findings demonstrate that let-7f-5p modulates lipogenesis by targeting SREBP2, providing novel insights into miRNA-mediated PRRSV pathogenesis and offering a potential antiviral therapeutic target. |
| format | Article |
| id | doaj-art-d4612e7deb3c45b7a636255750412cfe |
| institution | OA Journals |
| issn | 2306-7381 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj-art-d4612e7deb3c45b7a636255750412cfe2025-08-20T01:56:13ZengMDPI AGVeterinary Sciences2306-73812024-08-0111939210.3390/vetsci11090392Let-7f-5p Modulates Lipid Metabolism by Targeting Sterol Regulatory Element-Binding Protein 2 in Response to PRRSV InfectionDongfeng Jiang0Liyu Yang1Xiangge Meng2Qiuliang Xu3Xiang Zhou4Bang Liu5Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaPorcine reproductive and respiratory syndrome (PRRS) has caused substantial damage to the pig industry. MicroRNAs (miRNAs) were found to play crucial roles in modulating the pathogenesis of PRRS virus (PRRSV). In the present study, we revealed that PRRSV induced let-7f-5p to influence lipid metabolism to regulate PRRSV pathogenesis. A transcriptome analysis of PRRSV-infected PK15<sup>CD163</sup> cells transfected with let-7f-5p mimics or negative control (NC) generated 1718 differentially expressed genes, which were primarily associated with lipid metabolism processes. Furthermore, the master regulator of lipogenesis SREBP2 was found to be directly targeted by let-7f-5p using a dual-luciferase reporter system and Western blotting. The findings demonstrate that let-7f-5p modulates lipogenesis by targeting SREBP2, providing novel insights into miRNA-mediated PRRSV pathogenesis and offering a potential antiviral therapeutic target.https://www.mdpi.com/2306-7381/11/9/392let-7f-5pSREBP2PRRSVlipogenesisRNA-seq |
| spellingShingle | Dongfeng Jiang Liyu Yang Xiangge Meng Qiuliang Xu Xiang Zhou Bang Liu Let-7f-5p Modulates Lipid Metabolism by Targeting Sterol Regulatory Element-Binding Protein 2 in Response to PRRSV Infection Veterinary Sciences let-7f-5p SREBP2 PRRSV lipogenesis RNA-seq |
| title | Let-7f-5p Modulates Lipid Metabolism by Targeting Sterol Regulatory Element-Binding Protein 2 in Response to PRRSV Infection |
| title_full | Let-7f-5p Modulates Lipid Metabolism by Targeting Sterol Regulatory Element-Binding Protein 2 in Response to PRRSV Infection |
| title_fullStr | Let-7f-5p Modulates Lipid Metabolism by Targeting Sterol Regulatory Element-Binding Protein 2 in Response to PRRSV Infection |
| title_full_unstemmed | Let-7f-5p Modulates Lipid Metabolism by Targeting Sterol Regulatory Element-Binding Protein 2 in Response to PRRSV Infection |
| title_short | Let-7f-5p Modulates Lipid Metabolism by Targeting Sterol Regulatory Element-Binding Protein 2 in Response to PRRSV Infection |
| title_sort | let 7f 5p modulates lipid metabolism by targeting sterol regulatory element binding protein 2 in response to prrsv infection |
| topic | let-7f-5p SREBP2 PRRSV lipogenesis RNA-seq |
| url | https://www.mdpi.com/2306-7381/11/9/392 |
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