Development of a Mitochondrial Marker for Distinguishing Between <i>Ulva linza</i> and <i>Ulva prolifera</i>
The green tide is the biggest ecological disaster in Yellow Sea in recent decades, and the species composition has varied from year to year. The ITS (internal transcribed spacer) sequence combined with 5S rDNA spacer are the mainstream molecular markers for green macroalgae. The former can perfectly...
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MDPI AG
2025-03-01
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| Series: | Journal of Marine Science and Engineering |
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| Online Access: | https://www.mdpi.com/2077-1312/13/4/632 |
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| author | Rui Zhai Chengnuo Tang Shuying Wang Jing Ma Xinshu Li Guoqiang Chen Juntian Xu |
| author_facet | Rui Zhai Chengnuo Tang Shuying Wang Jing Ma Xinshu Li Guoqiang Chen Juntian Xu |
| author_sort | Rui Zhai |
| collection | DOAJ |
| description | The green tide is the biggest ecological disaster in Yellow Sea in recent decades, and the species composition has varied from year to year. The ITS (internal transcribed spacer) sequence combined with 5S rDNA spacer are the mainstream molecular markers for green macroalgae. The former can perfectly distinguish the majority of <i>Ulva</i> spp. except the <i>LPP</i> (<i>Ulva linza–procera–prolifera</i>) complex, and the latter is used to distinguish <i>U. linza</i> and <i>U. prolifera</i> exactly based on the former. However, in practice, 5S rDNA spacer is difficult to amplify perfectly with universal primers, and this ultimately affects the experimental process. For this reason, we developed a stable mitochondrial marker for the distinction between <i>U. linza</i> and <i>U. prolifera</i>. The phylogenetic tree based on the mitochondrial <i>rps2</i> (ribosomal protein S2) gene fragment can distinguish the <i>LPP</i> complex into two clades: <i>U. linza</i> and <i>U. prolifera.</i> Therefore, we concluded that the mitochondrial marker can be a great substitute for 5S primers to distinguish <i>U. linza</i> and <i>U. prolifera.</i> |
| format | Article |
| id | doaj-art-d46129cf9b27423fa7693fa023dd0935 |
| institution | DOAJ |
| issn | 2077-1312 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Marine Science and Engineering |
| spelling | doaj-art-d46129cf9b27423fa7693fa023dd09352025-08-20T03:13:51ZengMDPI AGJournal of Marine Science and Engineering2077-13122025-03-0113463210.3390/jmse13040632Development of a Mitochondrial Marker for Distinguishing Between <i>Ulva linza</i> and <i>Ulva prolifera</i>Rui Zhai0Chengnuo Tang1Shuying Wang2Jing Ma3Xinshu Li4Guoqiang Chen5Juntian Xu6Jiangsu Provincial Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Provincial Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaSchool of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Provincial Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Provincial Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Provincial Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Provincial Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaThe green tide is the biggest ecological disaster in Yellow Sea in recent decades, and the species composition has varied from year to year. The ITS (internal transcribed spacer) sequence combined with 5S rDNA spacer are the mainstream molecular markers for green macroalgae. The former can perfectly distinguish the majority of <i>Ulva</i> spp. except the <i>LPP</i> (<i>Ulva linza–procera–prolifera</i>) complex, and the latter is used to distinguish <i>U. linza</i> and <i>U. prolifera</i> exactly based on the former. However, in practice, 5S rDNA spacer is difficult to amplify perfectly with universal primers, and this ultimately affects the experimental process. For this reason, we developed a stable mitochondrial marker for the distinction between <i>U. linza</i> and <i>U. prolifera</i>. The phylogenetic tree based on the mitochondrial <i>rps2</i> (ribosomal protein S2) gene fragment can distinguish the <i>LPP</i> complex into two clades: <i>U. linza</i> and <i>U. prolifera.</i> Therefore, we concluded that the mitochondrial marker can be a great substitute for 5S primers to distinguish <i>U. linza</i> and <i>U. prolifera.</i>https://www.mdpi.com/2077-1312/13/4/632green algaemolecular identificationmitochondrial marker |
| spellingShingle | Rui Zhai Chengnuo Tang Shuying Wang Jing Ma Xinshu Li Guoqiang Chen Juntian Xu Development of a Mitochondrial Marker for Distinguishing Between <i>Ulva linza</i> and <i>Ulva prolifera</i> Journal of Marine Science and Engineering green algae molecular identification mitochondrial marker |
| title | Development of a Mitochondrial Marker for Distinguishing Between <i>Ulva linza</i> and <i>Ulva prolifera</i> |
| title_full | Development of a Mitochondrial Marker for Distinguishing Between <i>Ulva linza</i> and <i>Ulva prolifera</i> |
| title_fullStr | Development of a Mitochondrial Marker for Distinguishing Between <i>Ulva linza</i> and <i>Ulva prolifera</i> |
| title_full_unstemmed | Development of a Mitochondrial Marker for Distinguishing Between <i>Ulva linza</i> and <i>Ulva prolifera</i> |
| title_short | Development of a Mitochondrial Marker for Distinguishing Between <i>Ulva linza</i> and <i>Ulva prolifera</i> |
| title_sort | development of a mitochondrial marker for distinguishing between i ulva linza i and i ulva prolifera i |
| topic | green algae molecular identification mitochondrial marker |
| url | https://www.mdpi.com/2077-1312/13/4/632 |
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