A dispensable SepIVA orthologue in Streptomyces venezuelae is associated with polar growth and not cell division
Abstract Background SepIVA has been reported to be an essential septation factor in Mycolicibacterium smegmatis and Mycobacterium tuberculosis. It is a coiled-coil protein with similarity to DivIVA, a protein necessary for polar growth in members of the phylum Actinomycetota. Orthologues of SepIVA a...
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2024-11-01
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| Online Access: | https://doi.org/10.1186/s12866-024-03625-6 |
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| author | Beer Chakra Sen Parminder Singh Mavi Oihane Irazoki Susmita Datta Sebastian Kaiser Felipe Cava Klas Flärdh |
| author_facet | Beer Chakra Sen Parminder Singh Mavi Oihane Irazoki Susmita Datta Sebastian Kaiser Felipe Cava Klas Flärdh |
| author_sort | Beer Chakra Sen |
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| description | Abstract Background SepIVA has been reported to be an essential septation factor in Mycolicibacterium smegmatis and Mycobacterium tuberculosis. It is a coiled-coil protein with similarity to DivIVA, a protein necessary for polar growth in members of the phylum Actinomycetota. Orthologues of SepIVA are broadly distributed among actinomycetes, including in Streptomyces spp. Results To clarify the role of SepIVA and its potential involvement in cell division in streptomycetes, we generated sepIVA deletion mutants in Streptomyces venezuelae and found that sepIVA is dispensable for growth, cell division and sporulation. Further, mNeonGreen-SepIVA fusion protein did not localize at division septa, and we found no evidence of involvement of SepIVA in cell division. Instead, mNeonGreen-SepIVA was accumulated at the tips of growing vegetative hyphae in ways reminiscent of the apical localization of polarisome components like DivIVA. Bacterial two-hybrid system analyses revealed an interaction between SepIVA and DivIVA. The results indicate that SepIVA is associated with polar growth. However, no phenotypic effects of sepIVA deletion could be detected, and no evidence was observed of redundancy with the other DivIVA-like coiled-coil proteins Scy and FilP that are also associated with apical growth in streptomycetes. Conclusions We conclude that S. venezuelae SepIVA, in contrast to the situation in mycobacteria, is dispensable for growth and viability. The results suggest that it is associated with polar growth rather than septum formation. |
| format | Article |
| id | doaj-art-d444143c30f24831b4e5fb85c63e7f1b |
| institution | OA Journals |
| issn | 1471-2180 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
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| series | BMC Microbiology |
| spelling | doaj-art-d444143c30f24831b4e5fb85c63e7f1b2025-08-20T02:22:24ZengBMCBMC Microbiology1471-21802024-11-0124111510.1186/s12866-024-03625-6A dispensable SepIVA orthologue in Streptomyces venezuelae is associated with polar growth and not cell divisionBeer Chakra Sen0Parminder Singh Mavi1Oihane Irazoki2Susmita Datta3Sebastian Kaiser4Felipe Cava5Klas Flärdh6Department of Biology, Lund UniversityDepartment of Biology, Lund UniversityDepartment of Molecular Biology and Laboratory for Molecular Infection Medicine Sweden, Umeå Centre for Microbial Research, SciLifeLab, Umeå UniversityDepartment of Biology, Lund UniversityDepartment of Biology, Lund UniversityDepartment of Molecular Biology and Laboratory for Molecular Infection Medicine Sweden, Umeå Centre for Microbial Research, SciLifeLab, Umeå UniversityDepartment of Biology, Lund UniversityAbstract Background SepIVA has been reported to be an essential septation factor in Mycolicibacterium smegmatis and Mycobacterium tuberculosis. It is a coiled-coil protein with similarity to DivIVA, a protein necessary for polar growth in members of the phylum Actinomycetota. Orthologues of SepIVA are broadly distributed among actinomycetes, including in Streptomyces spp. Results To clarify the role of SepIVA and its potential involvement in cell division in streptomycetes, we generated sepIVA deletion mutants in Streptomyces venezuelae and found that sepIVA is dispensable for growth, cell division and sporulation. Further, mNeonGreen-SepIVA fusion protein did not localize at division septa, and we found no evidence of involvement of SepIVA in cell division. Instead, mNeonGreen-SepIVA was accumulated at the tips of growing vegetative hyphae in ways reminiscent of the apical localization of polarisome components like DivIVA. Bacterial two-hybrid system analyses revealed an interaction between SepIVA and DivIVA. The results indicate that SepIVA is associated with polar growth. However, no phenotypic effects of sepIVA deletion could be detected, and no evidence was observed of redundancy with the other DivIVA-like coiled-coil proteins Scy and FilP that are also associated with apical growth in streptomycetes. Conclusions We conclude that S. venezuelae SepIVA, in contrast to the situation in mycobacteria, is dispensable for growth and viability. The results suggest that it is associated with polar growth rather than septum formation.https://doi.org/10.1186/s12866-024-03625-6Cell divisionCell wall synthesisFtsZPolar growthStreptomyces |
| spellingShingle | Beer Chakra Sen Parminder Singh Mavi Oihane Irazoki Susmita Datta Sebastian Kaiser Felipe Cava Klas Flärdh A dispensable SepIVA orthologue in Streptomyces venezuelae is associated with polar growth and not cell division BMC Microbiology Cell division Cell wall synthesis FtsZ Polar growth Streptomyces |
| title | A dispensable SepIVA orthologue in Streptomyces venezuelae is associated with polar growth and not cell division |
| title_full | A dispensable SepIVA orthologue in Streptomyces venezuelae is associated with polar growth and not cell division |
| title_fullStr | A dispensable SepIVA orthologue in Streptomyces venezuelae is associated with polar growth and not cell division |
| title_full_unstemmed | A dispensable SepIVA orthologue in Streptomyces venezuelae is associated with polar growth and not cell division |
| title_short | A dispensable SepIVA orthologue in Streptomyces venezuelae is associated with polar growth and not cell division |
| title_sort | dispensable sepiva orthologue in streptomyces venezuelae is associated with polar growth and not cell division |
| topic | Cell division Cell wall synthesis FtsZ Polar growth Streptomyces |
| url | https://doi.org/10.1186/s12866-024-03625-6 |
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