Effect of eggshell calcium acetate on postmortem proteolysis and tenderization of spent duck muscle
Summary: The purpose of this study was to investigate the effect of eggshell calcium acetate on postmortem proteolysis and tenderization of Tsaiya duck muscle. Duck carcasses were obtained from a government-regulated slaughterhouse and chilled at 12 °C for 1 h. Both sides of the breast muscles were...
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Elsevier
2025-12-01
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| Series: | Journal of Applied Poultry Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1056617125000583 |
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| author | Ya-Shiou Chang |
| author_facet | Ya-Shiou Chang |
| author_sort | Ya-Shiou Chang |
| collection | DOAJ |
| description | Summary: The purpose of this study was to investigate the effect of eggshell calcium acetate on postmortem proteolysis and tenderization of Tsaiya duck muscle. Duck carcasses were obtained from a government-regulated slaughterhouse and chilled at 12 °C for 1 h. Both sides of the breast muscles were excised and incubated individually in either eggshell calcium acetate solution containing 30 mM Ca2+ (ECA, N = 15), 30 mM calcium acetate (ACA, N = 15), or 30 mM EDTA (EDTA, N = 15) at 5 °C for 3 days. The control group (CON, N = 15) was incubated in double-distillated water at 5 °C for 3 days. Breast muscle samples from the right side of each carcass were taken at 0 (1 h postmortem), 1, 2, and 3 days of incubation for pH determination, casein zymography, and Western blotting. Samples from the left side of each breast were taken for myofibrillar fragmentation index (MFI) and shear force measurements at 1 and 3 days of incubation. The results showed that the decrease in calpain-1 and calpain-11 activities, and desmin content was more rapid in the ECA- and ACA-incubated samples compared to the CON and EDTA-incubated samples during the 3-day incubation period. On the other hand, the MFI increased significantly in the ECA- and ACA-incubated samples compared to the CON and EDTA-incubated samples, while shear force values decreased more in the ECA- and ACA-incubated samples than in the CON and EDTA-incubated samples. Our results suggest that eggshell calcium acetate, like calcium acetate, can effectively enhance postmortem proteolysis and tenderizing of spent duck breast muscles. |
| format | Article |
| id | doaj-art-d442b37fdd134c6799fc85fc3391d1cd |
| institution | DOAJ |
| issn | 1056-6171 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Elsevier |
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| series | Journal of Applied Poultry Research |
| spelling | doaj-art-d442b37fdd134c6799fc85fc3391d1cd2025-08-20T02:49:29ZengElsevierJournal of Applied Poultry Research1056-61712025-12-0134410057410.1016/j.japr.2025.100574Effect of eggshell calcium acetate on postmortem proteolysis and tenderization of spent duck muscleYa-Shiou Chang0Department of Animal Science, Chinese Culture University, Taipei City, Taiwan; Department of Biotechnology and Animal Sciences, National Ilan University, Yilan County, TaiwanSummary: The purpose of this study was to investigate the effect of eggshell calcium acetate on postmortem proteolysis and tenderization of Tsaiya duck muscle. Duck carcasses were obtained from a government-regulated slaughterhouse and chilled at 12 °C for 1 h. Both sides of the breast muscles were excised and incubated individually in either eggshell calcium acetate solution containing 30 mM Ca2+ (ECA, N = 15), 30 mM calcium acetate (ACA, N = 15), or 30 mM EDTA (EDTA, N = 15) at 5 °C for 3 days. The control group (CON, N = 15) was incubated in double-distillated water at 5 °C for 3 days. Breast muscle samples from the right side of each carcass were taken at 0 (1 h postmortem), 1, 2, and 3 days of incubation for pH determination, casein zymography, and Western blotting. Samples from the left side of each breast were taken for myofibrillar fragmentation index (MFI) and shear force measurements at 1 and 3 days of incubation. The results showed that the decrease in calpain-1 and calpain-11 activities, and desmin content was more rapid in the ECA- and ACA-incubated samples compared to the CON and EDTA-incubated samples during the 3-day incubation period. On the other hand, the MFI increased significantly in the ECA- and ACA-incubated samples compared to the CON and EDTA-incubated samples, while shear force values decreased more in the ECA- and ACA-incubated samples than in the CON and EDTA-incubated samples. Our results suggest that eggshell calcium acetate, like calcium acetate, can effectively enhance postmortem proteolysis and tenderizing of spent duck breast muscles.http://www.sciencedirect.com/science/article/pii/S1056617125000583Spent duck musclePostmortem proteolysisTenderizationCalpain |
| spellingShingle | Ya-Shiou Chang Effect of eggshell calcium acetate on postmortem proteolysis and tenderization of spent duck muscle Journal of Applied Poultry Research Spent duck muscle Postmortem proteolysis Tenderization Calpain |
| title | Effect of eggshell calcium acetate on postmortem proteolysis and tenderization of spent duck muscle |
| title_full | Effect of eggshell calcium acetate on postmortem proteolysis and tenderization of spent duck muscle |
| title_fullStr | Effect of eggshell calcium acetate on postmortem proteolysis and tenderization of spent duck muscle |
| title_full_unstemmed | Effect of eggshell calcium acetate on postmortem proteolysis and tenderization of spent duck muscle |
| title_short | Effect of eggshell calcium acetate on postmortem proteolysis and tenderization of spent duck muscle |
| title_sort | effect of eggshell calcium acetate on postmortem proteolysis and tenderization of spent duck muscle |
| topic | Spent duck muscle Postmortem proteolysis Tenderization Calpain |
| url | http://www.sciencedirect.com/science/article/pii/S1056617125000583 |
| work_keys_str_mv | AT yashiouchang effectofeggshellcalciumacetateonpostmortemproteolysisandtenderizationofspentduckmuscle |