Behavioral endpoints and generational effects in Daphnia magna upon short- and long-term exposure and their use as additional endpoint in ecotoxicological risk assessment
Daphnia magna is a common model organism for ecotoxicological testing. In regulatory guidelines, the classical tested endpoints are immobilization and reproduction. In order to detect other sublethal effects potentially more sensitive endpoints are required. Behavioral endpoints are one approach in...
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Elsevier
2025-08-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325007729 |
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| author | Vanessa F. Saalmann Kirsten Germing Benedikt Ringbeck Lena A. Kosak Elke Eilebrecht |
| author_facet | Vanessa F. Saalmann Kirsten Germing Benedikt Ringbeck Lena A. Kosak Elke Eilebrecht |
| author_sort | Vanessa F. Saalmann |
| collection | DOAJ |
| description | Daphnia magna is a common model organism for ecotoxicological testing. In regulatory guidelines, the classical tested endpoints are immobilization and reproduction. In order to detect other sublethal effects potentially more sensitive endpoints are required. Behavioral endpoints are one approach in environmental risk assessment for more sensitive endpoints and monitoring of water quality, demonstrating ecological relevance since altered behavior can impact reproduction and survival, yet they are rarely included in risk assessment. In literature, several chemicals have shown to alter swimming behavior of D. magna. The objective of our study was to identify suitable behavioral endpoints regarding sensitivity and applicability under short-term and long-term exposure to selected insecticides in environmentally relevant concentrations and potential generational effects of insecticide exposure. The investigated endpoints were locomotor activity, spatial orientation and predator avoidance behavior. Neonate D. magna were exposed to the insecticides imidacloprid, thiacloprid and fipronil for 48 hours during short-term exposure and for 14 days during long-term exposure. In a second step, for the investigation of generational effects, behavioral alterations of neonates born under insecticide exposure were tested. The investigated insecticides altered the swimming patterns of D. magna, with statistically significant effects observed at lower concentrations compared to the classical endpoints immobility and reproduction. Thus, the current study demonstrates that the assessment of behavioral endpoints helps in informing Mode of Action (MoA)-specific risk assessment. Synopsis: Behavioral endpoints have recently drawn attention as more sensitive endpoints than regulatory relevant endpoints like immobilization or reproduction. This study identifies behavioral effects during short-term and long-term exposures and generational effects of insecticides with environmental relevance in the non-target organism Daphnia magna. |
| format | Article |
| id | doaj-art-d441fdb67dad42baa0540a23204f400a |
| institution | OA Journals |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-d441fdb67dad42baa0540a23204f400a2025-08-20T02:36:59ZengElsevierEcotoxicology and Environmental Safety0147-65132025-08-0130111843210.1016/j.ecoenv.2025.118432Behavioral endpoints and generational effects in Daphnia magna upon short- and long-term exposure and their use as additional endpoint in ecotoxicological risk assessmentVanessa F. Saalmann0Kirsten Germing1Benedikt Ringbeck2Lena A. Kosak3Elke Eilebrecht4Fraunhofer Institute for Molecular Biology and Applied Ecology, Schmallenberg, GermanyFraunhofer Institute for Molecular Biology and Applied Ecology, Schmallenberg, GermanyFraunhofer Institute for Molecular Biology and Applied Ecology, Schmallenberg, GermanyCorresponding author.; Fraunhofer Institute for Molecular Biology and Applied Ecology, Schmallenberg, GermanyFraunhofer Institute for Molecular Biology and Applied Ecology, Schmallenberg, GermanyDaphnia magna is a common model organism for ecotoxicological testing. In regulatory guidelines, the classical tested endpoints are immobilization and reproduction. In order to detect other sublethal effects potentially more sensitive endpoints are required. Behavioral endpoints are one approach in environmental risk assessment for more sensitive endpoints and monitoring of water quality, demonstrating ecological relevance since altered behavior can impact reproduction and survival, yet they are rarely included in risk assessment. In literature, several chemicals have shown to alter swimming behavior of D. magna. The objective of our study was to identify suitable behavioral endpoints regarding sensitivity and applicability under short-term and long-term exposure to selected insecticides in environmentally relevant concentrations and potential generational effects of insecticide exposure. The investigated endpoints were locomotor activity, spatial orientation and predator avoidance behavior. Neonate D. magna were exposed to the insecticides imidacloprid, thiacloprid and fipronil for 48 hours during short-term exposure and for 14 days during long-term exposure. In a second step, for the investigation of generational effects, behavioral alterations of neonates born under insecticide exposure were tested. The investigated insecticides altered the swimming patterns of D. magna, with statistically significant effects observed at lower concentrations compared to the classical endpoints immobility and reproduction. Thus, the current study demonstrates that the assessment of behavioral endpoints helps in informing Mode of Action (MoA)-specific risk assessment. Synopsis: Behavioral endpoints have recently drawn attention as more sensitive endpoints than regulatory relevant endpoints like immobilization or reproduction. This study identifies behavioral effects during short-term and long-term exposures and generational effects of insecticides with environmental relevance in the non-target organism Daphnia magna.http://www.sciencedirect.com/science/article/pii/S0147651325007729Daphnia magnaBehavioral ecotoxicologyBehavioral trackingToxicity assessmentSwimming behaviorSwimming parameters |
| spellingShingle | Vanessa F. Saalmann Kirsten Germing Benedikt Ringbeck Lena A. Kosak Elke Eilebrecht Behavioral endpoints and generational effects in Daphnia magna upon short- and long-term exposure and their use as additional endpoint in ecotoxicological risk assessment Ecotoxicology and Environmental Safety Daphnia magna Behavioral ecotoxicology Behavioral tracking Toxicity assessment Swimming behavior Swimming parameters |
| title | Behavioral endpoints and generational effects in Daphnia magna upon short- and long-term exposure and their use as additional endpoint in ecotoxicological risk assessment |
| title_full | Behavioral endpoints and generational effects in Daphnia magna upon short- and long-term exposure and their use as additional endpoint in ecotoxicological risk assessment |
| title_fullStr | Behavioral endpoints and generational effects in Daphnia magna upon short- and long-term exposure and their use as additional endpoint in ecotoxicological risk assessment |
| title_full_unstemmed | Behavioral endpoints and generational effects in Daphnia magna upon short- and long-term exposure and their use as additional endpoint in ecotoxicological risk assessment |
| title_short | Behavioral endpoints and generational effects in Daphnia magna upon short- and long-term exposure and their use as additional endpoint in ecotoxicological risk assessment |
| title_sort | behavioral endpoints and generational effects in daphnia magna upon short and long term exposure and their use as additional endpoint in ecotoxicological risk assessment |
| topic | Daphnia magna Behavioral ecotoxicology Behavioral tracking Toxicity assessment Swimming behavior Swimming parameters |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325007729 |
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