Cerebrospinal fluid immunoglobulin kappa light chain in clinically isolated syndrome and multiple sclerosis.
<h4>Background</h4>Oligoclonal bands (OCB) are the most widely used CSF test to support the diagnosis of MS and to predict conversion of clinically isolated syndrome (CIS) to multiple sclerosis (MS). Since OCB tests are based on non-quantitative and difficult to standardise techniques, m...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0088680&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850026276924948480 |
|---|---|
| author | Makbule Senel Hayrettin Tumani Florian Lauda Stefan Presslauer Rehaneh Mojib-Yezdani Markus Otto Johannes Brettschneider |
| author_facet | Makbule Senel Hayrettin Tumani Florian Lauda Stefan Presslauer Rehaneh Mojib-Yezdani Markus Otto Johannes Brettschneider |
| author_sort | Makbule Senel |
| collection | DOAJ |
| description | <h4>Background</h4>Oligoclonal bands (OCB) are the most widely used CSF test to support the diagnosis of MS and to predict conversion of clinically isolated syndrome (CIS) to multiple sclerosis (MS). Since OCB tests are based on non-quantitative and difficult to standardise techniques, measurement of immunoglobulin kappa free light chains (KFLC) may represent an easier to use quantitative test.<h4>Methods</h4>KFLC were measured in CSF and serum of 211 patients using ELISA. These include patients without any inflammatory central nervous system reaction (NIND, n = 77), MS (n = 20), viral CNS infections (V-CNS-I, n = 10), neuroborreliosis (NB, n = 17) and other bacterial CNS infections (B-CNS-I, n = 10). Furthermore a cohort of 77 patients with CIS, including 39 patients that remained CIS over follow-up of two years (CIS-CIS) and 38 patients that developed MS over the same follow-up time (CIS-MS).<h4>Results</h4>CSF-serum ratio of KFLC (Q KFLC) was elevated in all patients with MS, 86.8% of patients with CIS-MS and 61.5% of patients with CIS-CIS. It was significantly elevated in CIS with presence of OCB (p<0.001). Q KFLC significantly correlated with other CSF variables such as CSF leukocyte count (p<0.001, R = 0.46), CSF CXCL13 levels (p<0.001, R = 0.64) and also intrathecal IgG synthesis (p<0.001, R = 0.74) as determined by nephelometry and quotient diagram. OCB were detected in 66.7% of CIS-CIS and in 92.1% of CIS-MS.<h4>Conclusions</h4>Although the measurement of CSF KFLC is a rapid and quantitative easy to standardize tool, it is almost equal but not superior to OCB with regard to diagnostic sensitivity and specificity in patients with early MS. |
| format | Article |
| id | doaj-art-d4387e6939f146f8be4c4b0c73cd082e |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-d4387e6939f146f8be4c4b0c73cd082e2025-08-20T03:00:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e8868010.1371/journal.pone.0088680Cerebrospinal fluid immunoglobulin kappa light chain in clinically isolated syndrome and multiple sclerosis.Makbule SenelHayrettin TumaniFlorian LaudaStefan PresslauerRehaneh Mojib-YezdaniMarkus OttoJohannes Brettschneider<h4>Background</h4>Oligoclonal bands (OCB) are the most widely used CSF test to support the diagnosis of MS and to predict conversion of clinically isolated syndrome (CIS) to multiple sclerosis (MS). Since OCB tests are based on non-quantitative and difficult to standardise techniques, measurement of immunoglobulin kappa free light chains (KFLC) may represent an easier to use quantitative test.<h4>Methods</h4>KFLC were measured in CSF and serum of 211 patients using ELISA. These include patients without any inflammatory central nervous system reaction (NIND, n = 77), MS (n = 20), viral CNS infections (V-CNS-I, n = 10), neuroborreliosis (NB, n = 17) and other bacterial CNS infections (B-CNS-I, n = 10). Furthermore a cohort of 77 patients with CIS, including 39 patients that remained CIS over follow-up of two years (CIS-CIS) and 38 patients that developed MS over the same follow-up time (CIS-MS).<h4>Results</h4>CSF-serum ratio of KFLC (Q KFLC) was elevated in all patients with MS, 86.8% of patients with CIS-MS and 61.5% of patients with CIS-CIS. It was significantly elevated in CIS with presence of OCB (p<0.001). Q KFLC significantly correlated with other CSF variables such as CSF leukocyte count (p<0.001, R = 0.46), CSF CXCL13 levels (p<0.001, R = 0.64) and also intrathecal IgG synthesis (p<0.001, R = 0.74) as determined by nephelometry and quotient diagram. OCB were detected in 66.7% of CIS-CIS and in 92.1% of CIS-MS.<h4>Conclusions</h4>Although the measurement of CSF KFLC is a rapid and quantitative easy to standardize tool, it is almost equal but not superior to OCB with regard to diagnostic sensitivity and specificity in patients with early MS.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0088680&type=printable |
| spellingShingle | Makbule Senel Hayrettin Tumani Florian Lauda Stefan Presslauer Rehaneh Mojib-Yezdani Markus Otto Johannes Brettschneider Cerebrospinal fluid immunoglobulin kappa light chain in clinically isolated syndrome and multiple sclerosis. PLoS ONE |
| title | Cerebrospinal fluid immunoglobulin kappa light chain in clinically isolated syndrome and multiple sclerosis. |
| title_full | Cerebrospinal fluid immunoglobulin kappa light chain in clinically isolated syndrome and multiple sclerosis. |
| title_fullStr | Cerebrospinal fluid immunoglobulin kappa light chain in clinically isolated syndrome and multiple sclerosis. |
| title_full_unstemmed | Cerebrospinal fluid immunoglobulin kappa light chain in clinically isolated syndrome and multiple sclerosis. |
| title_short | Cerebrospinal fluid immunoglobulin kappa light chain in clinically isolated syndrome and multiple sclerosis. |
| title_sort | cerebrospinal fluid immunoglobulin kappa light chain in clinically isolated syndrome and multiple sclerosis |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0088680&type=printable |
| work_keys_str_mv | AT makbulesenel cerebrospinalfluidimmunoglobulinkappalightchaininclinicallyisolatedsyndromeandmultiplesclerosis AT hayrettintumani cerebrospinalfluidimmunoglobulinkappalightchaininclinicallyisolatedsyndromeandmultiplesclerosis AT florianlauda cerebrospinalfluidimmunoglobulinkappalightchaininclinicallyisolatedsyndromeandmultiplesclerosis AT stefanpresslauer cerebrospinalfluidimmunoglobulinkappalightchaininclinicallyisolatedsyndromeandmultiplesclerosis AT rehanehmojibyezdani cerebrospinalfluidimmunoglobulinkappalightchaininclinicallyisolatedsyndromeandmultiplesclerosis AT markusotto cerebrospinalfluidimmunoglobulinkappalightchaininclinicallyisolatedsyndromeandmultiplesclerosis AT johannesbrettschneider cerebrospinalfluidimmunoglobulinkappalightchaininclinicallyisolatedsyndromeandmultiplesclerosis |