The Functional and Prognostic Impact of TIGIT Expression on Bone Marrow NK Cells in Core Binding Factor-Acute Myeloid Leukemia Patients at Diagnosis
<b>Background</b>: The effect of the expression of the newly identified immune checkpoint, T cell immunoglobulin and immunoreceptor tyrosine-based inhibition motif domain (TIGIT) on NK cells in core binding factor-acute myeloid leukemia (CBF-AML) remains to be investigated. <b>Meth...
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2024-09-01
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| author | Dai-Hong Xie Jun Wang Kai Sun Zong-Yan Shi Ya-Zhe Wang Yan Chang Xiao-Ying Yuan Yan-Rong Liu Hao Jiang Qian Jiang Xiao-Jun Huang Ya-Zhen Qin |
| author_facet | Dai-Hong Xie Jun Wang Kai Sun Zong-Yan Shi Ya-Zhe Wang Yan Chang Xiao-Ying Yuan Yan-Rong Liu Hao Jiang Qian Jiang Xiao-Jun Huang Ya-Zhen Qin |
| author_sort | Dai-Hong Xie |
| collection | DOAJ |
| description | <b>Background</b>: The effect of the expression of the newly identified immune checkpoint, T cell immunoglobulin and immunoreceptor tyrosine-based inhibition motif domain (TIGIT) on NK cells in core binding factor-acute myeloid leukemia (CBF-AML) remains to be investigated. <b>Methods</b>: Fresh bone marrow samples from a total of 39 newly diagnosed CBF-AML patients and 25 healthy donors (HDs) were collected for testing the phenotype and function state of total NK, CD56<sup>bright</sup>, and CD56<sup>dim</sup> NK cell subsets after in vitro stimulation. <b>Results</b>: The frequencies of TIGIT<sup>+</sup> cells in total NK, CD56<sup>bright</sup>, and CD56<sup>dim</sup> NK cell subsets had no significant difference between patients and HDs. TNF-α and INF-γ levels were uniformly lower in TIGIT<sup>+</sup> cells than the corresponding TIGIT<sup>−</sup> cells in all HDs, whereas those for TIGIT<sup>+</sup> to TIGIT<sup>−</sup> cells in patients were highly heterogenous; TIGIT expression was not related to PFP and GZMB expression in HDs, whereas it was related to higher intracellular PFP and GZMB levels in patients. Patients’ TIGIT<sup>+</sup> NK cells displayed lower K562 cell-killing activity than their TIGIT<sup>−</sup> NK cells. In addition, high frequencies of TIGIT<sup>+</sup> cells in total NK and CD56<sup>dim</sup> NK cells were associated with poor RFS. <b>Conclusions</b>: TIGIT expression affected the diagnostic bone marrow-sited NK cell function and had prognostic significance in CBF-AML patients. |
| format | Article |
| id | doaj-art-d43435e1ff2f421e9a4d88869b95c731 |
| institution | OA Journals |
| issn | 2227-9059 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj-art-d43435e1ff2f421e9a4d88869b95c7312025-08-20T02:11:14ZengMDPI AGBiomedicines2227-90592024-09-011210220710.3390/biomedicines12102207The Functional and Prognostic Impact of TIGIT Expression on Bone Marrow NK Cells in Core Binding Factor-Acute Myeloid Leukemia Patients at DiagnosisDai-Hong Xie0Jun Wang1Kai Sun2Zong-Yan Shi3Ya-Zhe Wang4Yan Chang5Xiao-Ying Yuan6Yan-Rong Liu7Hao Jiang8Qian Jiang9Xiao-Jun Huang10Ya-Zhen Qin11Peking University People’s Hospital, Peking University Institute of Hematology, National Clinical Research Center for Hematologic Disease, Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation, Beijing 100044, ChinaPeking University People’s Hospital, Peking University Institute of Hematology, National Clinical Research Center for Hematologic Disease, Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation, Beijing 100044, ChinaPeking University People’s Hospital, Peking University Institute of Hematology, National Clinical Research Center for Hematologic Disease, Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation, Beijing 100044, ChinaPeking University People’s Hospital, Peking University Institute of Hematology, National Clinical Research Center for Hematologic Disease, Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation, Beijing 100044, ChinaPeking University People’s Hospital, Peking University Institute of Hematology, National Clinical Research Center for Hematologic Disease, Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation, Beijing 100044, ChinaPeking University People’s Hospital, Peking University Institute of Hematology, National Clinical Research Center for Hematologic Disease, Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation, Beijing 100044, ChinaPeking University People’s Hospital, Peking University Institute of Hematology, National Clinical Research Center for Hematologic Disease, Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation, Beijing 100044, ChinaPeking University People’s Hospital, Peking University Institute of Hematology, National Clinical Research Center for Hematologic Disease, Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation, Beijing 100044, ChinaPeking University People’s Hospital, Peking University Institute of Hematology, National Clinical Research Center for Hematologic Disease, Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation, Beijing 100044, ChinaPeking University People’s Hospital, Peking University Institute of Hematology, National Clinical Research Center for Hematologic Disease, Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation, Beijing 100044, ChinaPeking University People’s Hospital, Peking University Institute of Hematology, National Clinical Research Center for Hematologic Disease, Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation, Beijing 100044, ChinaPeking University People’s Hospital, Peking University Institute of Hematology, National Clinical Research Center for Hematologic Disease, Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation, Beijing 100044, China<b>Background</b>: The effect of the expression of the newly identified immune checkpoint, T cell immunoglobulin and immunoreceptor tyrosine-based inhibition motif domain (TIGIT) on NK cells in core binding factor-acute myeloid leukemia (CBF-AML) remains to be investigated. <b>Methods</b>: Fresh bone marrow samples from a total of 39 newly diagnosed CBF-AML patients and 25 healthy donors (HDs) were collected for testing the phenotype and function state of total NK, CD56<sup>bright</sup>, and CD56<sup>dim</sup> NK cell subsets after in vitro stimulation. <b>Results</b>: The frequencies of TIGIT<sup>+</sup> cells in total NK, CD56<sup>bright</sup>, and CD56<sup>dim</sup> NK cell subsets had no significant difference between patients and HDs. TNF-α and INF-γ levels were uniformly lower in TIGIT<sup>+</sup> cells than the corresponding TIGIT<sup>−</sup> cells in all HDs, whereas those for TIGIT<sup>+</sup> to TIGIT<sup>−</sup> cells in patients were highly heterogenous; TIGIT expression was not related to PFP and GZMB expression in HDs, whereas it was related to higher intracellular PFP and GZMB levels in patients. Patients’ TIGIT<sup>+</sup> NK cells displayed lower K562 cell-killing activity than their TIGIT<sup>−</sup> NK cells. In addition, high frequencies of TIGIT<sup>+</sup> cells in total NK and CD56<sup>dim</sup> NK cells were associated with poor RFS. <b>Conclusions</b>: TIGIT expression affected the diagnostic bone marrow-sited NK cell function and had prognostic significance in CBF-AML patients.https://www.mdpi.com/2227-9059/12/10/2207TIGITNK cell functioncore binding factor-acute myeloid leukemiaat diagnosisprognosisbone marrow |
| spellingShingle | Dai-Hong Xie Jun Wang Kai Sun Zong-Yan Shi Ya-Zhe Wang Yan Chang Xiao-Ying Yuan Yan-Rong Liu Hao Jiang Qian Jiang Xiao-Jun Huang Ya-Zhen Qin The Functional and Prognostic Impact of TIGIT Expression on Bone Marrow NK Cells in Core Binding Factor-Acute Myeloid Leukemia Patients at Diagnosis Biomedicines TIGIT NK cell function core binding factor-acute myeloid leukemia at diagnosis prognosis bone marrow |
| title | The Functional and Prognostic Impact of TIGIT Expression on Bone Marrow NK Cells in Core Binding Factor-Acute Myeloid Leukemia Patients at Diagnosis |
| title_full | The Functional and Prognostic Impact of TIGIT Expression on Bone Marrow NK Cells in Core Binding Factor-Acute Myeloid Leukemia Patients at Diagnosis |
| title_fullStr | The Functional and Prognostic Impact of TIGIT Expression on Bone Marrow NK Cells in Core Binding Factor-Acute Myeloid Leukemia Patients at Diagnosis |
| title_full_unstemmed | The Functional and Prognostic Impact of TIGIT Expression on Bone Marrow NK Cells in Core Binding Factor-Acute Myeloid Leukemia Patients at Diagnosis |
| title_short | The Functional and Prognostic Impact of TIGIT Expression on Bone Marrow NK Cells in Core Binding Factor-Acute Myeloid Leukemia Patients at Diagnosis |
| title_sort | functional and prognostic impact of tigit expression on bone marrow nk cells in core binding factor acute myeloid leukemia patients at diagnosis |
| topic | TIGIT NK cell function core binding factor-acute myeloid leukemia at diagnosis prognosis bone marrow |
| url | https://www.mdpi.com/2227-9059/12/10/2207 |
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