Diospyros mespilifoemis hochst Modulates the Hippocampus and Prefrontal Cortex of Wistar Rat following Lithium chloride pilocarpine-induced Epilepsy
Objectives: Epilepsy is a disease with vast complexity and diverse clinical manifestations causing neuronal misfiring. Diospyros mespiliformis (DM) possesses anti-epileptic properties. Methods: Fifty rats were used for this study. Group A received normal saline while groups B, C, D, and E receive...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Babcock Medical Society
2024-12-01
|
| Series: | Babcock University Medical Journal |
| Subjects: | |
| Online Access: | http://bumj.babcock.edu.ng/index.php/bumj/article/view/347 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850048463375433728 |
|---|---|
| author | Sunday Yinka Olatunji Oluwafemi Ezekiel Kale Oluseyi Abimbola Ogunsola John Afees Olanrewaju Ijeoma Esiaba Taiye Adelodun Olubunmi Philip Ogunnaike |
| author_facet | Sunday Yinka Olatunji Oluwafemi Ezekiel Kale Oluseyi Abimbola Ogunsola John Afees Olanrewaju Ijeoma Esiaba Taiye Adelodun Olubunmi Philip Ogunnaike |
| author_sort | Sunday Yinka Olatunji |
| collection | DOAJ |
| description |
Objectives: Epilepsy is a disease with vast complexity and diverse clinical manifestations causing neuronal misfiring. Diospyros mespiliformis (DM) possesses anti-epileptic properties.
Methods: Fifty rats were used for this study. Group A received normal saline while groups B, C, D, and E received lithium chloride (127 mg/kg, i.p.) + pilocarpine (30 mg/kg, i.p.) to induce seizure. Following induction, C, D, and E received 50 mg/kg, 200 mg/kg DM and 10 mg/kg sodium valproate respectively (p.o) while B was left untreated. Rats were assessed for Open field and radial arm maze tests. Levels of SOD, MDA, catalase glutamate, GABA, IL-6 and TNF-alpha were assayed using appropriate protocols. Histological and immunohistochemistry tests were done.
Results: DM reduced glutamate levels in C, D and E when compared with A and B while there were no significant changes in the levels of GABA across groups. DM significantly reduced IL-6 in C, D and E but not in A and B while there were no significant changes in TNF alpha across groups. DM boosted catalase release than superoxide dismutase to the after-status epilepticus. In the open field test, DM reversed the altered activities in the epileptic rats. DM ameliorates neuronal vacuolation, disorientation and increased reactive gliosis. There was positive NeuN reactivity across groups except in D.
Conclusion: We concluded that DM (probably 1,2,3-Benzenetriol content) can be employed in managing epilepsy as evident in the mitigation of histoarchitecture and the maintenance of the levels of the neurotransmitters.
|
| format | Article |
| id | doaj-art-d433598f674b4232ae7cbaf6bd3db06b |
| institution | DOAJ |
| issn | 2465-6666 2756-4657 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Babcock Medical Society |
| record_format | Article |
| series | Babcock University Medical Journal |
| spelling | doaj-art-d433598f674b4232ae7cbaf6bd3db06b2025-08-20T02:53:57ZengBabcock Medical SocietyBabcock University Medical Journal2465-66662756-46572024-12-017210.38029/babcockuniv.med.j..v7i2.347Diospyros mespilifoemis hochst Modulates the Hippocampus and Prefrontal Cortex of Wistar Rat following Lithium chloride pilocarpine-induced EpilepsySunday Yinka Olatunji0Oluwafemi Ezekiel Kale1Oluseyi Abimbola Ogunsola2John Afees Olanrewaju3Ijeoma Esiaba4Taiye Adelodun5Olubunmi Philip Ogunnaike6Department of Anatomy, Adventist School of Medicine of East Central Africa, Adventist University of Central Africa, Kigali, RwandaDepartment of Pharmacology and Therapeutics, Faculty of Basic Medical Sciences, Olabisi Onabanjo University, Ago-Iwoye, Sagamu Campus, Ogun State, P.M.B. 2001 Ikeja, Nigeria.Department of Physiology, Benjamin S. Carson College of Health and Medical Sciences, Babcock University, NigeriaDepartment of Anatomy, Benjamin S. Carson College of Health and Medical Sciences, Babcock University, NigeriaDepartment of Biochemistry, Benjamin S. Carson College of Health and Medical Sciences, Babcock University, NigeriaDepartment of Anatomy, Benjamin S. Carson College of Health and Medical Sciences, Babcock University, NigeriaDepartment of Anatomy, Benjamin S. Carson College of Health and Medical Sciences, Babcock University, Nigeria Objectives: Epilepsy is a disease with vast complexity and diverse clinical manifestations causing neuronal misfiring. Diospyros mespiliformis (DM) possesses anti-epileptic properties. Methods: Fifty rats were used for this study. Group A received normal saline while groups B, C, D, and E received lithium chloride (127 mg/kg, i.p.) + pilocarpine (30 mg/kg, i.p.) to induce seizure. Following induction, C, D, and E received 50 mg/kg, 200 mg/kg DM and 10 mg/kg sodium valproate respectively (p.o) while B was left untreated. Rats were assessed for Open field and radial arm maze tests. Levels of SOD, MDA, catalase glutamate, GABA, IL-6 and TNF-alpha were assayed using appropriate protocols. Histological and immunohistochemistry tests were done. Results: DM reduced glutamate levels in C, D and E when compared with A and B while there were no significant changes in the levels of GABA across groups. DM significantly reduced IL-6 in C, D and E but not in A and B while there were no significant changes in TNF alpha across groups. DM boosted catalase release than superoxide dismutase to the after-status epilepticus. In the open field test, DM reversed the altered activities in the epileptic rats. DM ameliorates neuronal vacuolation, disorientation and increased reactive gliosis. There was positive NeuN reactivity across groups except in D. Conclusion: We concluded that DM (probably 1,2,3-Benzenetriol content) can be employed in managing epilepsy as evident in the mitigation of histoarchitecture and the maintenance of the levels of the neurotransmitters. http://bumj.babcock.edu.ng/index.php/bumj/article/view/347EpilepsyLiCl-pilocarpineAnimal ModelDiospyros mespiliformisImmunohistochemistry |
| spellingShingle | Sunday Yinka Olatunji Oluwafemi Ezekiel Kale Oluseyi Abimbola Ogunsola John Afees Olanrewaju Ijeoma Esiaba Taiye Adelodun Olubunmi Philip Ogunnaike Diospyros mespilifoemis hochst Modulates the Hippocampus and Prefrontal Cortex of Wistar Rat following Lithium chloride pilocarpine-induced Epilepsy Babcock University Medical Journal Epilepsy LiCl-pilocarpine Animal Model Diospyros mespiliformis Immunohistochemistry |
| title | Diospyros mespilifoemis hochst Modulates the Hippocampus and Prefrontal Cortex of Wistar Rat following Lithium chloride pilocarpine-induced Epilepsy |
| title_full | Diospyros mespilifoemis hochst Modulates the Hippocampus and Prefrontal Cortex of Wistar Rat following Lithium chloride pilocarpine-induced Epilepsy |
| title_fullStr | Diospyros mespilifoemis hochst Modulates the Hippocampus and Prefrontal Cortex of Wistar Rat following Lithium chloride pilocarpine-induced Epilepsy |
| title_full_unstemmed | Diospyros mespilifoemis hochst Modulates the Hippocampus and Prefrontal Cortex of Wistar Rat following Lithium chloride pilocarpine-induced Epilepsy |
| title_short | Diospyros mespilifoemis hochst Modulates the Hippocampus and Prefrontal Cortex of Wistar Rat following Lithium chloride pilocarpine-induced Epilepsy |
| title_sort | diospyros mespilifoemis hochst modulates the hippocampus and prefrontal cortex of wistar rat following lithium chloride pilocarpine induced epilepsy |
| topic | Epilepsy LiCl-pilocarpine Animal Model Diospyros mespiliformis Immunohistochemistry |
| url | http://bumj.babcock.edu.ng/index.php/bumj/article/view/347 |
| work_keys_str_mv | AT sundayyinkaolatunji diospyrosmespilifoemishochstmodulatesthehippocampusandprefrontalcortexofwistarratfollowinglithiumchloridepilocarpineinducedepilepsy AT oluwafemiezekielkale diospyrosmespilifoemishochstmodulatesthehippocampusandprefrontalcortexofwistarratfollowinglithiumchloridepilocarpineinducedepilepsy AT oluseyiabimbolaogunsola diospyrosmespilifoemishochstmodulatesthehippocampusandprefrontalcortexofwistarratfollowinglithiumchloridepilocarpineinducedepilepsy AT johnafeesolanrewaju diospyrosmespilifoemishochstmodulatesthehippocampusandprefrontalcortexofwistarratfollowinglithiumchloridepilocarpineinducedepilepsy AT ijeomaesiaba diospyrosmespilifoemishochstmodulatesthehippocampusandprefrontalcortexofwistarratfollowinglithiumchloridepilocarpineinducedepilepsy AT taiyeadelodun diospyrosmespilifoemishochstmodulatesthehippocampusandprefrontalcortexofwistarratfollowinglithiumchloridepilocarpineinducedepilepsy AT olubunmiphilipogunnaike diospyrosmespilifoemishochstmodulatesthehippocampusandprefrontalcortexofwistarratfollowinglithiumchloridepilocarpineinducedepilepsy |