Superior normalization using total protein for western blot analysis of human adipocytes.
Western blotting has been a pivotal method for analyzing protein expression since the late 1970s, yet there is no established consensus on an optimal normalization strategy. In this study, primary mature human adipocytes were used to investigate the robustness of housekeeping proteins and total prot...
Saved in:
| Main Authors: | Leo J S Westerberg, Benjamin Dedic, Erik Näslund, Anders Thorell, Kirsty L Spalding |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2025-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0328136 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Superior normalization using total protein for western blot analysis of human adipocytes
by: Leo J.S. Westerberg, et al.
Published: (2025-01-01) -
Total protein analysis as a reliable loading control for quantitative fluorescent Western blotting.
by: Samantha L Eaton, et al.
Published: (2013-01-01) -
Western blotting of native proteins from agarose gels
by: Chiaki Sakuma, et al.
Published: (2022-05-01) -
Chemiluminescent Detection of Protein Molecular Weight Markers in Western Blot Techniques
by: Rui Hai Liu, et al.
Published: (1997-04-01) -
Improved Specificity of Western Blot Following Processing at 4°C Using BlotCycler
Published: (2017-05-01)