Effect of Bacillus velezensis on the structure of the rhizosphere microbial community and yield of soybean
Abstract The beneficial effects of Bacillus on crop growth have been confirmed in various contexts. However, the impact of exogenous Bacillus application on the rhizosphere microbial community throughout the entire growth cycle of soybean remains unclear. This study investigated the soybean cultivar...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-08-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-025-07048-x |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849333461178908672 |
|---|---|
| author | Dongxue Lv Lei Zhang Yuanhang Guo Fengnian Zhao Zhenxi Cao Shiva Kumar Jha Djifa Fidele Kpalari Xingpeng Wang |
| author_facet | Dongxue Lv Lei Zhang Yuanhang Guo Fengnian Zhao Zhenxi Cao Shiva Kumar Jha Djifa Fidele Kpalari Xingpeng Wang |
| author_sort | Dongxue Lv |
| collection | DOAJ |
| description | Abstract The beneficial effects of Bacillus on crop growth have been confirmed in various contexts. However, the impact of exogenous Bacillus application on the rhizosphere microbial community throughout the entire growth cycle of soybean remains unclear. This study investigated the soybean cultivar ‘Qihuang 34’ under three gradient treatments: Control, no Bacillus application, 0 kg/ha(CK), low Bacillus application, 15 kg/ha(BL), and high Bacillus application, 30 kg/ha(BH), analyzing the dynamic changes in rhizosphere soil microbial community structure and their effects on yield across different growth stages (vegetative(V2), flowering(R2), fruiting(R4) and pod-filling(R6)). The results showed that bacterial α-diversity indices were generally higher at the vegetative stage than at flowering, pod-setting, and seed-filling stages, with inconsistent variation patterns across growth periods. The relative abundance of dominant bacterial phyla and genera varied over time. In the BL treatment, the relative abundances of Rhizobiaceae, Sphingomonadaceae, and Pseudomonadaceae significantly increased at the R4 and R6 stages (p < 0.05). In the BH treatment, the relative abundance of Fusarium decreased by 16.16% (R4) and 65.21% (R6) compared to CK. Correlation analysis between soil physicochemical properties and microbial phylum-level composition revealed that total nitrogen (TN) and total sulfur (TS) were the primary drivers of rhizosphere bacterial communities, while TS played a key role in shaping fungal communities. Additionally, the BH treatment significantly increased soybean yield by 4.50–13.84% (p < 0.05). This study provides foundational insights into how Bacillus influences the composition and diversity of soybean rhizosphere microbial communities, supporting the development of sustainable agricultural practices through rhizosphere microbiome management. |
| format | Article |
| id | doaj-art-d43105adfa0e4c9bbbd26225fe5c2833 |
| institution | Kabale University |
| issn | 1471-2229 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-d43105adfa0e4c9bbbd26225fe5c28332025-08-20T03:45:51ZengBMCBMC Plant Biology1471-22292025-08-0125111610.1186/s12870-025-07048-xEffect of Bacillus velezensis on the structure of the rhizosphere microbial community and yield of soybeanDongxue Lv0Lei Zhang1Yuanhang Guo2Fengnian Zhao3Zhenxi Cao4Shiva Kumar Jha5Djifa Fidele Kpalari6Xingpeng Wang7College of Water Hydraulic and Architectural Engineering, Tarim UniversityCollege of Water Hydraulic and Architectural Engineering, Tarim UniversityCollege of Water Hydraulic and Architectural Engineering, Tarim UniversityCollege of Water Hydraulic and Architectural Engineering, Tarim UniversityCollege of Water Hydraulic and Architectural Engineering, Tarim UniversityNepal Agricultural Research Council, National Agricultural Engineering Research CentreKey Laboratory of Crop Water Use and Regulation, Ministry of Agriculture and Rural Affairs/Institute of Farmland Irrigation of CAASCollege of Water Hydraulic and Architectural Engineering, Tarim UniversityAbstract The beneficial effects of Bacillus on crop growth have been confirmed in various contexts. However, the impact of exogenous Bacillus application on the rhizosphere microbial community throughout the entire growth cycle of soybean remains unclear. This study investigated the soybean cultivar ‘Qihuang 34’ under three gradient treatments: Control, no Bacillus application, 0 kg/ha(CK), low Bacillus application, 15 kg/ha(BL), and high Bacillus application, 30 kg/ha(BH), analyzing the dynamic changes in rhizosphere soil microbial community structure and their effects on yield across different growth stages (vegetative(V2), flowering(R2), fruiting(R4) and pod-filling(R6)). The results showed that bacterial α-diversity indices were generally higher at the vegetative stage than at flowering, pod-setting, and seed-filling stages, with inconsistent variation patterns across growth periods. The relative abundance of dominant bacterial phyla and genera varied over time. In the BL treatment, the relative abundances of Rhizobiaceae, Sphingomonadaceae, and Pseudomonadaceae significantly increased at the R4 and R6 stages (p < 0.05). In the BH treatment, the relative abundance of Fusarium decreased by 16.16% (R4) and 65.21% (R6) compared to CK. Correlation analysis between soil physicochemical properties and microbial phylum-level composition revealed that total nitrogen (TN) and total sulfur (TS) were the primary drivers of rhizosphere bacterial communities, while TS played a key role in shaping fungal communities. Additionally, the BH treatment significantly increased soybean yield by 4.50–13.84% (p < 0.05). This study provides foundational insights into how Bacillus influences the composition and diversity of soybean rhizosphere microbial communities, supporting the development of sustainable agricultural practices through rhizosphere microbiome management.https://doi.org/10.1186/s12870-025-07048-xSoybean (Glycine max(L.) Merr.)Rhizosphere microorganismsCommunity structureBacillus amendmentDiversity |
| spellingShingle | Dongxue Lv Lei Zhang Yuanhang Guo Fengnian Zhao Zhenxi Cao Shiva Kumar Jha Djifa Fidele Kpalari Xingpeng Wang Effect of Bacillus velezensis on the structure of the rhizosphere microbial community and yield of soybean BMC Plant Biology Soybean (Glycine max(L.) Merr.) Rhizosphere microorganisms Community structure Bacillus amendment Diversity |
| title | Effect of Bacillus velezensis on the structure of the rhizosphere microbial community and yield of soybean |
| title_full | Effect of Bacillus velezensis on the structure of the rhizosphere microbial community and yield of soybean |
| title_fullStr | Effect of Bacillus velezensis on the structure of the rhizosphere microbial community and yield of soybean |
| title_full_unstemmed | Effect of Bacillus velezensis on the structure of the rhizosphere microbial community and yield of soybean |
| title_short | Effect of Bacillus velezensis on the structure of the rhizosphere microbial community and yield of soybean |
| title_sort | effect of bacillus velezensis on the structure of the rhizosphere microbial community and yield of soybean |
| topic | Soybean (Glycine max(L.) Merr.) Rhizosphere microorganisms Community structure Bacillus amendment Diversity |
| url | https://doi.org/10.1186/s12870-025-07048-x |
| work_keys_str_mv | AT dongxuelv effectofbacillusvelezensisonthestructureoftherhizospheremicrobialcommunityandyieldofsoybean AT leizhang effectofbacillusvelezensisonthestructureoftherhizospheremicrobialcommunityandyieldofsoybean AT yuanhangguo effectofbacillusvelezensisonthestructureoftherhizospheremicrobialcommunityandyieldofsoybean AT fengnianzhao effectofbacillusvelezensisonthestructureoftherhizospheremicrobialcommunityandyieldofsoybean AT zhenxicao effectofbacillusvelezensisonthestructureoftherhizospheremicrobialcommunityandyieldofsoybean AT shivakumarjha effectofbacillusvelezensisonthestructureoftherhizospheremicrobialcommunityandyieldofsoybean AT djifafidelekpalari effectofbacillusvelezensisonthestructureoftherhizospheremicrobialcommunityandyieldofsoybean AT xingpengwang effectofbacillusvelezensisonthestructureoftherhizospheremicrobialcommunityandyieldofsoybean |