Separation and Identification of Furocoumarin in Fruits of Heracleum candicans DC. by HPTLC
Heracleum candicans Wall. ex DC. (Apiaceae) is widely used in Indian system of medicines as an aphrodisiac, nerve tonic and also in the treatment of skin diseases with reports of being rich in furocoumarins which are extensively used in pharmaceutical industry for their photosensitizing activity on...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/915762 |
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| author | A. K. S. Rawat A. P. Singh D. P. Singh M. M. Pandey R. Govindarajan Sharad Srivastava |
| author_facet | A. K. S. Rawat A. P. Singh D. P. Singh M. M. Pandey R. Govindarajan Sharad Srivastava |
| author_sort | A. K. S. Rawat |
| collection | DOAJ |
| description | Heracleum candicans Wall. ex DC. (Apiaceae) is widely used in Indian system of medicines as an aphrodisiac, nerve tonic and also in the treatment of skin diseases with reports of being rich in furocoumarins which are extensively used in pharmaceutical industry for their photosensitizing activity on human skin. A simple high performance thin layer chromatography (HPTLC) method has been developed for the simultaneous determination of psoralen (1) and heraclenol (2) in the fruits of H. candicans. The analytes were separated on silica gel F254 plates with toluene : diethy lether : acetic acid (6 : 4 : 1) and scanned densitometrically at 350 nm. The method was validated in terms of precision, repeatability, and accuracy. The linear range for psoralen and heraclenol was found to be 1–5 μg spot−1 and 1–4 μg spot−1 with correlation coefficient of 0.973 and 0.964, respectively. The two compounds were quantified in fruits of H. candicans and were found to be present in the range of 0.021–0.036% and 0.029–0.043% w/w. The method was found to be very simple, accurate, precise, and economical and can be used for routine quality control. |
| format | Article |
| id | doaj-art-d42ce58f4f5b40f78ad916682ffeb154 |
| institution | DOAJ |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-d42ce58f4f5b40f78ad916682ffeb1542025-08-20T03:23:47ZengWileyJournal of Chemistry2090-90632090-90712013-01-01201310.1155/2013/915762915762Separation and Identification of Furocoumarin in Fruits of Heracleum candicans DC. by HPTLCA. K. S. Rawat0A. P. Singh1D. P. Singh2M. M. Pandey3R. Govindarajan4Sharad Srivastava5Pharmacognosy and Ethnopharmacology Division, CSIR-National Botanical Research Institute, Lucknow 226003, IndiaPharmacognosy and Ethnopharmacology Division, CSIR-National Botanical Research Institute, Lucknow 226003, IndiaPharmacognosy and Ethnopharmacology Division, CSIR-National Botanical Research Institute, Lucknow 226003, IndiaPharmacognosy and Ethnopharmacology Division, CSIR-National Botanical Research Institute, Lucknow 226003, IndiaPharmacognosy and Ethnopharmacology Division, CSIR-National Botanical Research Institute, Lucknow 226003, IndiaPharmacognosy and Ethnopharmacology Division, CSIR-National Botanical Research Institute, Lucknow 226003, IndiaHeracleum candicans Wall. ex DC. (Apiaceae) is widely used in Indian system of medicines as an aphrodisiac, nerve tonic and also in the treatment of skin diseases with reports of being rich in furocoumarins which are extensively used in pharmaceutical industry for their photosensitizing activity on human skin. A simple high performance thin layer chromatography (HPTLC) method has been developed for the simultaneous determination of psoralen (1) and heraclenol (2) in the fruits of H. candicans. The analytes were separated on silica gel F254 plates with toluene : diethy lether : acetic acid (6 : 4 : 1) and scanned densitometrically at 350 nm. The method was validated in terms of precision, repeatability, and accuracy. The linear range for psoralen and heraclenol was found to be 1–5 μg spot−1 and 1–4 μg spot−1 with correlation coefficient of 0.973 and 0.964, respectively. The two compounds were quantified in fruits of H. candicans and were found to be present in the range of 0.021–0.036% and 0.029–0.043% w/w. The method was found to be very simple, accurate, precise, and economical and can be used for routine quality control.http://dx.doi.org/10.1155/2013/915762 |
| spellingShingle | A. K. S. Rawat A. P. Singh D. P. Singh M. M. Pandey R. Govindarajan Sharad Srivastava Separation and Identification of Furocoumarin in Fruits of Heracleum candicans DC. by HPTLC Journal of Chemistry |
| title | Separation and Identification of Furocoumarin in Fruits of Heracleum candicans DC. by HPTLC |
| title_full | Separation and Identification of Furocoumarin in Fruits of Heracleum candicans DC. by HPTLC |
| title_fullStr | Separation and Identification of Furocoumarin in Fruits of Heracleum candicans DC. by HPTLC |
| title_full_unstemmed | Separation and Identification of Furocoumarin in Fruits of Heracleum candicans DC. by HPTLC |
| title_short | Separation and Identification of Furocoumarin in Fruits of Heracleum candicans DC. by HPTLC |
| title_sort | separation and identification of furocoumarin in fruits of heracleum candicans dc by hptlc |
| url | http://dx.doi.org/10.1155/2013/915762 |
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