Identification of Differentially Expressed Gene after Femoral Fracture via Microarray Profiling
We aimed to investigate differentially expressed genes (DEGs) in different stages after femoral fracture based on rat models, providing the basis for the treatment of sport-related fractures. Gene expression data GSE3298 was downloaded from Gene Expression Omnibus (GEO), including 16 chips. All femo...
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| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2014/208751 |
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| author | Donggen Zhong |
| author_facet | Donggen Zhong |
| author_sort | Donggen Zhong |
| collection | DOAJ |
| description | We aimed to investigate differentially expressed genes (DEGs) in different stages after femoral fracture based on rat models, providing the basis for the treatment of sport-related fractures. Gene expression data GSE3298 was downloaded from Gene Expression Omnibus (GEO), including 16 chips. All femoral fracture samples were classified into earlier fracture stage and later fracture stage. Total 87 DEGs simultaneously occurred in two stages, of which 4 genes showed opposite expression tendency. Out of the 4 genes, Rest and Cst8 were hub nodes in protein-protein interaction (PPI) network. The GO (Gene Ontology) function enrichment analysis verified that nutrition supply related genes were enriched in the earlier stage and neuron growth related genes were enriched in the later stage. Calcium signaling pathway was the most significant pathway in earlier stage; in later stage, DEGs were enriched into 2 neurodevelopment-related pathways. Analysis of Pearson's correlation coefficient showed that a total of 3,300 genes were significantly associated with fracture time, none of which was overlapped with identified DEGs. This study suggested that Rest and Cst8 might act as potential indicators for fracture healing. Calcium signaling pathway and neurodevelopment-related pathways might be deeply involved in bone healing after femoral fracture. |
| format | Article |
| id | doaj-art-d41894ee48a94e418ab16c1e77b0bcf4 |
| institution | DOAJ |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-d41894ee48a94e418ab16c1e77b0bcf42025-08-20T03:23:47ZengWileyInternational Journal of Genomics2314-436X2314-43782014-01-01201410.1155/2014/208751208751Identification of Differentially Expressed Gene after Femoral Fracture via Microarray ProfilingDonggen Zhong0Department of Physical Education, Jiangxi University of Finance and Economics, Nanchang 330013, ChinaWe aimed to investigate differentially expressed genes (DEGs) in different stages after femoral fracture based on rat models, providing the basis for the treatment of sport-related fractures. Gene expression data GSE3298 was downloaded from Gene Expression Omnibus (GEO), including 16 chips. All femoral fracture samples were classified into earlier fracture stage and later fracture stage. Total 87 DEGs simultaneously occurred in two stages, of which 4 genes showed opposite expression tendency. Out of the 4 genes, Rest and Cst8 were hub nodes in protein-protein interaction (PPI) network. The GO (Gene Ontology) function enrichment analysis verified that nutrition supply related genes were enriched in the earlier stage and neuron growth related genes were enriched in the later stage. Calcium signaling pathway was the most significant pathway in earlier stage; in later stage, DEGs were enriched into 2 neurodevelopment-related pathways. Analysis of Pearson's correlation coefficient showed that a total of 3,300 genes were significantly associated with fracture time, none of which was overlapped with identified DEGs. This study suggested that Rest and Cst8 might act as potential indicators for fracture healing. Calcium signaling pathway and neurodevelopment-related pathways might be deeply involved in bone healing after femoral fracture.http://dx.doi.org/10.1155/2014/208751 |
| spellingShingle | Donggen Zhong Identification of Differentially Expressed Gene after Femoral Fracture via Microarray Profiling International Journal of Genomics |
| title | Identification of Differentially Expressed Gene after Femoral Fracture via Microarray Profiling |
| title_full | Identification of Differentially Expressed Gene after Femoral Fracture via Microarray Profiling |
| title_fullStr | Identification of Differentially Expressed Gene after Femoral Fracture via Microarray Profiling |
| title_full_unstemmed | Identification of Differentially Expressed Gene after Femoral Fracture via Microarray Profiling |
| title_short | Identification of Differentially Expressed Gene after Femoral Fracture via Microarray Profiling |
| title_sort | identification of differentially expressed gene after femoral fracture via microarray profiling |
| url | http://dx.doi.org/10.1155/2014/208751 |
| work_keys_str_mv | AT donggenzhong identificationofdifferentiallyexpressedgeneafterfemoralfractureviamicroarrayprofiling |