Phytochemical screening and anti-Helicobacter pylori activity of Bryophyllum pinnatum, Ocimum gratissimum and Vernonia amygdalina
Helicobacter pylori, a common gastrointestinal bacterial pathogenic isolate infects 50% and 90% of the global and developing nations population respectively. This study aimed at evaluating the bioactive components and therapeutic potential of Bryophyllum pinnatum, Ocimum gratissimum and Vernonia amy...
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Plovdiv University Press
2025-01-01
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| Series: | Journal of BioScience and Biotechnology |
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| Online Access: | https://editorial.uni-plovdiv.bg/index.php/JBB/article/view/533/389 |
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| author | Precious Egharevba Emilia Igue Kingsley Enerijiofi Orhue Amengialue |
| author_facet | Precious Egharevba Emilia Igue Kingsley Enerijiofi Orhue Amengialue |
| author_sort | Precious Egharevba |
| collection | DOAJ |
| description | Helicobacter pylori, a common gastrointestinal bacterial pathogenic isolate infects 50% and 90% of the global and developing nations population respectively. This study aimed at evaluating the bioactive components and therapeutic potential of Bryophyllum pinnatum, Ocimum gratissimum and Vernonia amygdalina plants extracts against Helicobacter pylori infection using standard physicochemical, in-vitro and in-vivo microbiological methods. Five (5) stool samples were collected from patients who presented with symptoms of gastrointestinal distress and diagnosed of ulcer at the University of Benin Teaching Hospital, for the isolation of Helicobacter pylori. Phytochemical screening of ethanol extract of the test plants revealed the presence of bioactive constituents such as flavonoids, tannins, cardiac glycosides, saponin, steroids, phenols, alkaloids and terpenoids. The minimum inhibitory concentration (MIC) of the plant’s extracts were determined at concentrations of 125, 250, 500, and 1000 μg/ml. While the different plants extract demonstrated a better anti-Helicobacter pylori activity as well as MIC when compared to the commonly used antibiotic amoxicillin, and other conventional antibiotics, the anti-Helicobacter pylori activity and MIC of Vernonia amygdalina was higher, followed by Bryophyllum pinnatum and Ocimum gratissimum. The in-vivo study as carried out using Wistar albino rats demonstrated the promising therapeutic effect of the plants extract against Helicobacter pylori infection. This study therefore suggests that Vernonia amygdalina, Bryophyllum pinnatum and Ocimum gratissimum extracts possess anti-Helicobacter pylori properties, offering safe, effective, and cost-effective treatment options for the treatment of ulcer, caused by Helicobacter pylori. |
| format | Article |
| id | doaj-art-d4131f06b92f40e5ad0dc3861056b5f5 |
| institution | Kabale University |
| issn | 1314-6238 1314-6246 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Plovdiv University Press |
| record_format | Article |
| series | Journal of BioScience and Biotechnology |
| spelling | doaj-art-d4131f06b92f40e5ad0dc3861056b5f52025-01-28T07:42:22ZengPlovdiv University PressJournal of BioScience and Biotechnology1314-62381314-62462025-01-0113216317110.69085/jbb20242163Phytochemical screening and anti-Helicobacter pylori activity of Bryophyllum pinnatum, Ocimum gratissimum and Vernonia amygdalinaPrecious Egharevba0Emilia Igue1Kingsley Enerijiofi2Orhue Amengialue3Department of Biological Sciences, College of Science and Computing, Wellspring University, Benin City, Edo State, NigeriaDepartment of Biological Sciences, College of Science and Computing, Wellspring University, Benin City, Edo State, NigeriaDepartment of Biological Sciences, College of Basic and Applied Sciences, Glorious Vision University (formerly Samuel Adegboyega University), Ogwa, Edo State, NigeriaDepartment of Biological Sciences, College of Basic and Applied Sciences, Glorious Vision University (formerly Samuel Adegboyega University), Ogwa, Edo State, NigeriaHelicobacter pylori, a common gastrointestinal bacterial pathogenic isolate infects 50% and 90% of the global and developing nations population respectively. This study aimed at evaluating the bioactive components and therapeutic potential of Bryophyllum pinnatum, Ocimum gratissimum and Vernonia amygdalina plants extracts against Helicobacter pylori infection using standard physicochemical, in-vitro and in-vivo microbiological methods. Five (5) stool samples were collected from patients who presented with symptoms of gastrointestinal distress and diagnosed of ulcer at the University of Benin Teaching Hospital, for the isolation of Helicobacter pylori. Phytochemical screening of ethanol extract of the test plants revealed the presence of bioactive constituents such as flavonoids, tannins, cardiac glycosides, saponin, steroids, phenols, alkaloids and terpenoids. The minimum inhibitory concentration (MIC) of the plant’s extracts were determined at concentrations of 125, 250, 500, and 1000 μg/ml. While the different plants extract demonstrated a better anti-Helicobacter pylori activity as well as MIC when compared to the commonly used antibiotic amoxicillin, and other conventional antibiotics, the anti-Helicobacter pylori activity and MIC of Vernonia amygdalina was higher, followed by Bryophyllum pinnatum and Ocimum gratissimum. The in-vivo study as carried out using Wistar albino rats demonstrated the promising therapeutic effect of the plants extract against Helicobacter pylori infection. This study therefore suggests that Vernonia amygdalina, Bryophyllum pinnatum and Ocimum gratissimum extracts possess anti-Helicobacter pylori properties, offering safe, effective, and cost-effective treatment options for the treatment of ulcer, caused by Helicobacter pylori.https://editorial.uni-plovdiv.bg/index.php/JBB/article/view/533/389antimicrobialextracthelicobacter pyloriphytochemicalvernonia amygdalina |
| spellingShingle | Precious Egharevba Emilia Igue Kingsley Enerijiofi Orhue Amengialue Phytochemical screening and anti-Helicobacter pylori activity of Bryophyllum pinnatum, Ocimum gratissimum and Vernonia amygdalina Journal of BioScience and Biotechnology antimicrobial extract helicobacter pylori phytochemical vernonia amygdalina |
| title | Phytochemical screening and anti-Helicobacter pylori activity of Bryophyllum pinnatum, Ocimum gratissimum and Vernonia amygdalina |
| title_full | Phytochemical screening and anti-Helicobacter pylori activity of Bryophyllum pinnatum, Ocimum gratissimum and Vernonia amygdalina |
| title_fullStr | Phytochemical screening and anti-Helicobacter pylori activity of Bryophyllum pinnatum, Ocimum gratissimum and Vernonia amygdalina |
| title_full_unstemmed | Phytochemical screening and anti-Helicobacter pylori activity of Bryophyllum pinnatum, Ocimum gratissimum and Vernonia amygdalina |
| title_short | Phytochemical screening and anti-Helicobacter pylori activity of Bryophyllum pinnatum, Ocimum gratissimum and Vernonia amygdalina |
| title_sort | phytochemical screening and anti helicobacter pylori activity of bryophyllum pinnatum ocimum gratissimum and vernonia amygdalina |
| topic | antimicrobial extract helicobacter pylori phytochemical vernonia amygdalina |
| url | https://editorial.uni-plovdiv.bg/index.php/JBB/article/view/533/389 |
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