Effects of Trichomonas gallinae infection and diet on blood microbiome composition in european greenfinches (Chloris chloris)

Recent research has reported microbial invasion of the bloodstream in various disease-associated conditions. In this study, we investigated the role of trichomonosis outbreak (caused by the Trichomonas gallinae parasite) and food availability in shaping the blood microbiome composition of wintering...

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Main Authors: Tatjana Krama, Ronalds Krams, Sergejs Popovs, Dita Gudrā, Maija Ustinova, Dāvids Fridmanis, Giedrius Trakimas, Jorge Contreras-Garduño, Dina Cīrule, Markus J. Rantala, Colton B. Adams, Priit Jõers, Indrikis A. Krams
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-06-01
Series:Frontiers in Physiology
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Online Access:https://www.frontiersin.org/articles/10.3389/fphys.2025.1576833/full
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author Tatjana Krama
Tatjana Krama
Tatjana Krama
Ronalds Krams
Ronalds Krams
Ronalds Krams
Sergejs Popovs
Dita Gudrā
Maija Ustinova
Dāvids Fridmanis
Giedrius Trakimas
Giedrius Trakimas
Jorge Contreras-Garduño
Dina Cīrule
Dina Cīrule
Markus J. Rantala
Colton B. Adams
Colton B. Adams
Colton B. Adams
Colton B. Adams
Priit Jõers
Indrikis A. Krams
Indrikis A. Krams
Indrikis A. Krams
Indrikis A. Krams
author_facet Tatjana Krama
Tatjana Krama
Tatjana Krama
Ronalds Krams
Ronalds Krams
Ronalds Krams
Sergejs Popovs
Dita Gudrā
Maija Ustinova
Dāvids Fridmanis
Giedrius Trakimas
Giedrius Trakimas
Jorge Contreras-Garduño
Dina Cīrule
Dina Cīrule
Markus J. Rantala
Colton B. Adams
Colton B. Adams
Colton B. Adams
Colton B. Adams
Priit Jõers
Indrikis A. Krams
Indrikis A. Krams
Indrikis A. Krams
Indrikis A. Krams
author_sort Tatjana Krama
collection DOAJ
description Recent research has reported microbial invasion of the bloodstream in various disease-associated conditions. In this study, we investigated the role of trichomonosis outbreak (caused by the Trichomonas gallinae parasite) and food availability in shaping the blood microbiome composition of wintering greenfinches (Chloris chloris). Data were collected during two periods: before the outbreak (December) and during the outbreak (February). No bacterial contamination was observed in pre-epidemic blood samples. All individuals were infected during the outbreak, but greenfinches with irregular food access exhibited lower bacterial contamination in their blood. Individuals with permanent food access had a greater proportional representation of specific microbial taxa and higher alpha diversity in their blood microbiomes. However, beta diversity did not differ between the two groups. We demonstrated that trichomonosis infection and feeding regime play critical roles in mediating septic conditions of peripheral circulation during an outbreak, with food accessibility influencing blood microbial contamination. These findings integrate the impacts of feeding regimes and hematological responses to improve our understanding of the complex interactions between diet, disease, and physiological resilience in wild birds.
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publisher Frontiers Media S.A.
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spelling doaj-art-d40ce3e3d2bb43809d197b31a4b8e38c2025-08-20T03:07:45ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2025-06-011610.3389/fphys.2025.15768331576833Effects of Trichomonas gallinae infection and diet on blood microbiome composition in european greenfinches (Chloris chloris)Tatjana Krama0Tatjana Krama1Tatjana Krama2Ronalds Krams3Ronalds Krams4Ronalds Krams5Sergejs Popovs6Dita Gudrā7Maija Ustinova8Dāvids Fridmanis9Giedrius Trakimas10Giedrius Trakimas11Jorge Contreras-Garduño12Dina Cīrule13Dina Cīrule14Markus J. Rantala15Colton B. Adams16Colton B. Adams17Colton B. Adams18Colton B. Adams19Priit Jõers20Indrikis A. Krams21Indrikis A. Krams22Indrikis A. Krams23Indrikis A. Krams24Department of Biodiversity, Institute of Life Sciences and Technology, Daugavpils University, Daugavpils, LatviaChair of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Tartu, EstoniaLatvian Biomedical Research and Study Centre, Riga, LatviaDepartment of Biodiversity, Institute of Life Sciences and Technology, Daugavpils University, Daugavpils, LatviaChair of Plant Health, Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Tartu, EstoniaInstitute of Food Safety, Animal Health and Environment “BIOR”, Riga, LatviaDepartment of Biodiversity, Institute of Life Sciences and Technology, Daugavpils University, Daugavpils, LatviaLatvian Biomedical Research and Study Centre, Riga, LatviaLatvian Biomedical Research and Study Centre, Riga, LatviaLatvian Biomedical Research and Study Centre, Riga, LatviaDepartment of Biodiversity, Institute of Life Sciences and Technology, Daugavpils University, Daugavpils, LatviaInstitute of Biosciences, Life Sciences Center, Vilnius University, Vilnius, LithuaniaEscuela Nacional de Estudios Superiores, National Autonomous University of Mexico, Morelia, MexicoInstitute of Food Safety, Animal Health and Environment “BIOR”, Riga, LatviaFaculty of Veterinary Medicine, Latvia University of Life Sciences and Technologies, Jelgava, LatviaDepartment of Biology & Turku Brain and Mind Centre, University of Turku, Turku, FinlandDepartment of Biodiversity, Institute of Life Sciences and Technology, Daugavpils University, Daugavpils, LatviaLatvian Biomedical Research and Study Centre, Riga, LatviaDepartment of Ecology and Evolutionary Biology, University of Tennessee, Knoxville, TN, United States0Department of Psychology, University of Tennessee, Knoxville, TN, United States1Institute of Molecular and Cell Biology, University of Tartu, Tartu, EstoniaDepartment of Biodiversity, Institute of Life Sciences and Technology, Daugavpils University, Daugavpils, LatviaLatvian Biomedical Research and Study Centre, Riga, Latvia2Department of Ecology, University of Latvia, Riga, Latvia3Institute of Ecology and Earth Sciences, University of Tartu, Tartu, EstoniaRecent research has reported microbial invasion of the bloodstream in various disease-associated conditions. In this study, we investigated the role of trichomonosis outbreak (caused by the Trichomonas gallinae parasite) and food availability in shaping the blood microbiome composition of wintering greenfinches (Chloris chloris). Data were collected during two periods: before the outbreak (December) and during the outbreak (February). No bacterial contamination was observed in pre-epidemic blood samples. All individuals were infected during the outbreak, but greenfinches with irregular food access exhibited lower bacterial contamination in their blood. Individuals with permanent food access had a greater proportional representation of specific microbial taxa and higher alpha diversity in their blood microbiomes. However, beta diversity did not differ between the two groups. We demonstrated that trichomonosis infection and feeding regime play critical roles in mediating septic conditions of peripheral circulation during an outbreak, with food accessibility influencing blood microbial contamination. These findings integrate the impacts of feeding regimes and hematological responses to improve our understanding of the complex interactions between diet, disease, and physiological resilience in wild birds.https://www.frontiersin.org/articles/10.3389/fphys.2025.1576833/fullblood microbiomeChloris chlorisEuropean greenfinchbird feeding behaviorhealth and disease ecologytrichomonosis outbreaks
spellingShingle Tatjana Krama
Tatjana Krama
Tatjana Krama
Ronalds Krams
Ronalds Krams
Ronalds Krams
Sergejs Popovs
Dita Gudrā
Maija Ustinova
Dāvids Fridmanis
Giedrius Trakimas
Giedrius Trakimas
Jorge Contreras-Garduño
Dina Cīrule
Dina Cīrule
Markus J. Rantala
Colton B. Adams
Colton B. Adams
Colton B. Adams
Colton B. Adams
Priit Jõers
Indrikis A. Krams
Indrikis A. Krams
Indrikis A. Krams
Indrikis A. Krams
Effects of Trichomonas gallinae infection and diet on blood microbiome composition in european greenfinches (Chloris chloris)
Frontiers in Physiology
blood microbiome
Chloris chloris
European greenfinch
bird feeding behavior
health and disease ecology
trichomonosis outbreaks
title Effects of Trichomonas gallinae infection and diet on blood microbiome composition in european greenfinches (Chloris chloris)
title_full Effects of Trichomonas gallinae infection and diet on blood microbiome composition in european greenfinches (Chloris chloris)
title_fullStr Effects of Trichomonas gallinae infection and diet on blood microbiome composition in european greenfinches (Chloris chloris)
title_full_unstemmed Effects of Trichomonas gallinae infection and diet on blood microbiome composition in european greenfinches (Chloris chloris)
title_short Effects of Trichomonas gallinae infection and diet on blood microbiome composition in european greenfinches (Chloris chloris)
title_sort effects of trichomonas gallinae infection and diet on blood microbiome composition in european greenfinches chloris chloris
topic blood microbiome
Chloris chloris
European greenfinch
bird feeding behavior
health and disease ecology
trichomonosis outbreaks
url https://www.frontiersin.org/articles/10.3389/fphys.2025.1576833/full
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