Could Increased Glp1r Expression via Sitagliptin in the GLP-1/GLP-1 Receptor Axis in the DietInduced Obesity Rat Model be Important in Liver Metabolism?
Objective: The aim of this study was to evaluate the contribution of sitagliptin, which is used in the treatment of type 2 diabetes mellitus due to its insulinotropic effects, to the levels of glucagon-like peptide-1 (GLP-1) expressed in many systemic tissues in obesity, in liver, skeletal muscle, a...
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Istanbul University Press
2024-12-01
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| Online Access: | https://dergipark.org.tr/en/download/article-file/4282885 |
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| author | Cem Horozoğlu Serkan Gürgül Gülper Nacarkahya Neslihan Cevik |
| author_facet | Cem Horozoğlu Serkan Gürgül Gülper Nacarkahya Neslihan Cevik |
| author_sort | Cem Horozoğlu |
| collection | DOAJ |
| description | Objective: The aim of this study was to evaluate the contribution of sitagliptin, which is used in the treatment of type 2 diabetes mellitus due to its insulinotropic effects, to the levels of glucagon-like peptide-1 (GLP-1) expressed in many systemic tissues in obesity, in liver, skeletal muscle, and fat tissue.Materials and Methods: Adult Wistar albino rats (n=32) were randomly divided into four groups for 16 weeks of intervention. These groups were control (C) (n=8), obese (Ob) (n=8), sitagliptin (C+Stg) (n=8), and obese (Ob+Stg) given sitagliptin (n=8). Glp1r expression in rat liver, muscle, and adipose tissue was confirmed by quantitative real-time PCR.Results: No significant change was detected in Glp1r expression levels in muscle and fat tissue in 4 groups. A 10.64-fold increase in Glp1r gene expression was observed in Ob compared to C (p=0.008). Additionally, a 4.03-fold increase in expression level was found in Ob+Stg compared to Ob (p=0.02) and a 12.52-fold increase in expression level was found in Ob+Stg compared to C (p=0.01).Conclusion: The increased Glp1r expression intensity in obese individuals using sitagliptin compared with controls and obese individuals not using sitagliptin may play a role in the reorganization of liver metabolism that is impaired due to obesity, such as the gluconeogenesis process. |
| format | Article |
| id | doaj-art-d40a67006adf4d39889174522e361a2f |
| institution | Kabale University |
| issn | 2667-5846 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Istanbul University Press |
| record_format | Article |
| series | Experimed |
| spelling | doaj-art-d40a67006adf4d39889174522e361a2f2025-08-20T03:34:45ZengIstanbul University PressExperimed2667-58462024-12-0114321021410.26650/experimed.15659624Could Increased Glp1r Expression via Sitagliptin in the GLP-1/GLP-1 Receptor Axis in the DietInduced Obesity Rat Model be Important in Liver Metabolism?Cem Horozoğlu0https://orcid.org/0000-0001-8998-2028Serkan Gürgül1https://orcid.org/0000-0002-1450-490XGülper Nacarkahya2https://orcid.org/0000-0002-8512-8833Neslihan Cevik3https://orcid.org/0000-0001-9631-8221Department of Medical Biochemistry, Faculty of Medicine, Halic UniversityDepartment of Biophysics, Faculty of Medicine, Gaziantep UniversityDepartment of Medical Biology, Faculty of Medicine, Gaziantep UniversityInstitute of Health Sciences, Gaziantep UniversityObjective: The aim of this study was to evaluate the contribution of sitagliptin, which is used in the treatment of type 2 diabetes mellitus due to its insulinotropic effects, to the levels of glucagon-like peptide-1 (GLP-1) expressed in many systemic tissues in obesity, in liver, skeletal muscle, and fat tissue.Materials and Methods: Adult Wistar albino rats (n=32) were randomly divided into four groups for 16 weeks of intervention. These groups were control (C) (n=8), obese (Ob) (n=8), sitagliptin (C+Stg) (n=8), and obese (Ob+Stg) given sitagliptin (n=8). Glp1r expression in rat liver, muscle, and adipose tissue was confirmed by quantitative real-time PCR.Results: No significant change was detected in Glp1r expression levels in muscle and fat tissue in 4 groups. A 10.64-fold increase in Glp1r gene expression was observed in Ob compared to C (p=0.008). Additionally, a 4.03-fold increase in expression level was found in Ob+Stg compared to Ob (p=0.02) and a 12.52-fold increase in expression level was found in Ob+Stg compared to C (p=0.01).Conclusion: The increased Glp1r expression intensity in obese individuals using sitagliptin compared with controls and obese individuals not using sitagliptin may play a role in the reorganization of liver metabolism that is impaired due to obesity, such as the gluconeogenesis process.https://dergipark.org.tr/en/download/article-file/4282885sitagliptinglp-1obesityliver metabolismglp1rglp1r |
| spellingShingle | Cem Horozoğlu Serkan Gürgül Gülper Nacarkahya Neslihan Cevik Could Increased Glp1r Expression via Sitagliptin in the GLP-1/GLP-1 Receptor Axis in the DietInduced Obesity Rat Model be Important in Liver Metabolism? Experimed sitagliptin glp-1 obesity liver metabolism glp1r glp1r |
| title | Could Increased Glp1r Expression via Sitagliptin in the GLP-1/GLP-1 Receptor Axis in the DietInduced Obesity Rat Model be Important in Liver Metabolism? |
| title_full | Could Increased Glp1r Expression via Sitagliptin in the GLP-1/GLP-1 Receptor Axis in the DietInduced Obesity Rat Model be Important in Liver Metabolism? |
| title_fullStr | Could Increased Glp1r Expression via Sitagliptin in the GLP-1/GLP-1 Receptor Axis in the DietInduced Obesity Rat Model be Important in Liver Metabolism? |
| title_full_unstemmed | Could Increased Glp1r Expression via Sitagliptin in the GLP-1/GLP-1 Receptor Axis in the DietInduced Obesity Rat Model be Important in Liver Metabolism? |
| title_short | Could Increased Glp1r Expression via Sitagliptin in the GLP-1/GLP-1 Receptor Axis in the DietInduced Obesity Rat Model be Important in Liver Metabolism? |
| title_sort | could increased glp1r expression via sitagliptin in the glp 1 glp 1 receptor axis in the dietinduced obesity rat model be important in liver metabolism |
| topic | sitagliptin glp-1 obesity liver metabolism glp1r glp1r |
| url | https://dergipark.org.tr/en/download/article-file/4282885 |
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