Temperature Influences Antimicrobial Resistance and Virulence of <i>Vibrio parahaemolyticus</i> Clinical Isolates from Quebec, Canada
Climate change and ocean warming have a huge impact on microbial communities, leading to an increased prevalence of <i>Vibrio parahaemolyticus</i> infections in northern America. <i>V. parahaemolyticus</i> is an inhabitant of aquatic environments and is associated with fishes...
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MDPI AG
2025-05-01
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| author | Feriel C. Mahieddine Annabelle Mathieu-Denoncourt Marylise Duperthuy |
| author_facet | Feriel C. Mahieddine Annabelle Mathieu-Denoncourt Marylise Duperthuy |
| author_sort | Feriel C. Mahieddine |
| collection | DOAJ |
| description | Climate change and ocean warming have a huge impact on microbial communities, leading to an increased prevalence of <i>Vibrio parahaemolyticus</i> infections in northern America. <i>V. parahaemolyticus</i> is an inhabitant of aquatic environments and is associated with fishes and shellfishes, particularly with oysters. It causes gastrointestinal infection through consumption of contaminated seafood, as well as wound infections or septicemia. Temperature is known to affect virulence and persistence factors in <i>V. parahaemolyticus</i>. In this study, twenty clinical strains isolated form sick patients in Quebec, Canada, were characterized for persistence and virulence factor production at different temperatures to assess the impact of a switch from sea water to the human body on them. Their capacity to produce biofilm, hemolysins, and membrane vesicles as well as their motility and antibiotic resistance at 20 °C and 37 °C were assessed. Our results revealed that while temperature had little effect on vesicle production, it significantly influenced their growth, antimicrobial resistance, biofilm formation, and motility. Additionally, all <i>V. parahaemolyticus</i> strains produced hemolysins at 37 °C but not under environmental conditions. |
| format | Article |
| id | doaj-art-d402fbeb8f334da49fd5594eb5533eaa |
| institution | Kabale University |
| issn | 2076-0817 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj-art-d402fbeb8f334da49fd5594eb5533eaa2025-08-20T03:27:32ZengMDPI AGPathogens2076-08172025-05-0114652110.3390/pathogens14060521Temperature Influences Antimicrobial Resistance and Virulence of <i>Vibrio parahaemolyticus</i> Clinical Isolates from Quebec, CanadaFeriel C. Mahieddine0Annabelle Mathieu-Denoncourt1Marylise Duperthuy2Département de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université de Montréal, Montréal, QC H3T 1J4, CanadaDépartement de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université de Montréal, Montréal, QC H3T 1J4, CanadaDépartement de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université de Montréal, Montréal, QC H3T 1J4, CanadaClimate change and ocean warming have a huge impact on microbial communities, leading to an increased prevalence of <i>Vibrio parahaemolyticus</i> infections in northern America. <i>V. parahaemolyticus</i> is an inhabitant of aquatic environments and is associated with fishes and shellfishes, particularly with oysters. It causes gastrointestinal infection through consumption of contaminated seafood, as well as wound infections or septicemia. Temperature is known to affect virulence and persistence factors in <i>V. parahaemolyticus</i>. In this study, twenty clinical strains isolated form sick patients in Quebec, Canada, were characterized for persistence and virulence factor production at different temperatures to assess the impact of a switch from sea water to the human body on them. Their capacity to produce biofilm, hemolysins, and membrane vesicles as well as their motility and antibiotic resistance at 20 °C and 37 °C were assessed. Our results revealed that while temperature had little effect on vesicle production, it significantly influenced their growth, antimicrobial resistance, biofilm formation, and motility. Additionally, all <i>V. parahaemolyticus</i> strains produced hemolysins at 37 °C but not under environmental conditions.https://www.mdpi.com/2076-0817/14/6/521<i>Vibrio parahaemolyticus</i>virulencebiofilmmotilityhemolysinantimicrobial resistance |
| spellingShingle | Feriel C. Mahieddine Annabelle Mathieu-Denoncourt Marylise Duperthuy Temperature Influences Antimicrobial Resistance and Virulence of <i>Vibrio parahaemolyticus</i> Clinical Isolates from Quebec, Canada Pathogens <i>Vibrio parahaemolyticus</i> virulence biofilm motility hemolysin antimicrobial resistance |
| title | Temperature Influences Antimicrobial Resistance and Virulence of <i>Vibrio parahaemolyticus</i> Clinical Isolates from Quebec, Canada |
| title_full | Temperature Influences Antimicrobial Resistance and Virulence of <i>Vibrio parahaemolyticus</i> Clinical Isolates from Quebec, Canada |
| title_fullStr | Temperature Influences Antimicrobial Resistance and Virulence of <i>Vibrio parahaemolyticus</i> Clinical Isolates from Quebec, Canada |
| title_full_unstemmed | Temperature Influences Antimicrobial Resistance and Virulence of <i>Vibrio parahaemolyticus</i> Clinical Isolates from Quebec, Canada |
| title_short | Temperature Influences Antimicrobial Resistance and Virulence of <i>Vibrio parahaemolyticus</i> Clinical Isolates from Quebec, Canada |
| title_sort | temperature influences antimicrobial resistance and virulence of i vibrio parahaemolyticus i clinical isolates from quebec canada |
| topic | <i>Vibrio parahaemolyticus</i> virulence biofilm motility hemolysin antimicrobial resistance |
| url | https://www.mdpi.com/2076-0817/14/6/521 |
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